全文获取类型
收费全文 | 1466篇 |
免费 | 72篇 |
国内免费 | 11篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 29篇 |
妇产科学 | 6篇 |
基础医学 | 200篇 |
口腔科学 | 10篇 |
临床医学 | 70篇 |
内科学 | 365篇 |
皮肤病学 | 43篇 |
神经病学 | 80篇 |
特种医学 | 75篇 |
外科学 | 205篇 |
综合类 | 5篇 |
预防医学 | 28篇 |
眼科学 | 1篇 |
药学 | 140篇 |
中国医学 | 4篇 |
肿瘤学 | 287篇 |
出版年
2023年 | 12篇 |
2022年 | 13篇 |
2021年 | 32篇 |
2020年 | 17篇 |
2019年 | 19篇 |
2018年 | 46篇 |
2017年 | 25篇 |
2016年 | 29篇 |
2015年 | 22篇 |
2014年 | 49篇 |
2013年 | 41篇 |
2012年 | 92篇 |
2011年 | 81篇 |
2010年 | 34篇 |
2009年 | 32篇 |
2008年 | 58篇 |
2007年 | 62篇 |
2006年 | 69篇 |
2005年 | 83篇 |
2004年 | 83篇 |
2003年 | 80篇 |
2002年 | 67篇 |
2001年 | 50篇 |
2000年 | 54篇 |
1999年 | 55篇 |
1998年 | 32篇 |
1997年 | 15篇 |
1996年 | 20篇 |
1995年 | 19篇 |
1994年 | 12篇 |
1993年 | 17篇 |
1992年 | 35篇 |
1991年 | 25篇 |
1990年 | 25篇 |
1989年 | 19篇 |
1988年 | 20篇 |
1987年 | 13篇 |
1986年 | 18篇 |
1985年 | 6篇 |
1984年 | 6篇 |
1983年 | 9篇 |
1982年 | 3篇 |
1981年 | 8篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1971年 | 4篇 |
1970年 | 5篇 |
1969年 | 7篇 |
1968年 | 4篇 |
1922年 | 2篇 |
排序方式: 共有1549条查询结果,搜索用时 0 毫秒
91.
Hasegawa H Katano H Tanno M Masuo S Ae T Sato Y Takahashi H Iwasaki T Kurata T Sata T 《Leukemia & lymphoma》2004,45(10):2169-2172
We report a case of BCL-6-positive B cell lymphoma with human herpesvirus 8 (HHV-8) infection. A human immunodeficiency virus-infected patient developed a diffuse large B cell lymphoma, which was found exclusively in the liver and spleen with the absence of lymphadenopathy and effusion in any body cavities. The lymphoma cells were composed of medium to large-sized cells positive for CD20, CD45, and BCL-6, and negative for epithelial cell membrane antigen, CD30, CD45RO, and CD138/syndecan-1, suggesting a germinal center B cell origin. The patient was serologically positive for HHV-8, and HHV-8 was detected in the liver biopsy tissue both by polymerase chain reaction and by immunohistochemistry for HHV-8-encoded latency-associated nuclear antigen. Other HHV-8-associated diseases, such as Kaposi's sarcoma, primary effusion lymphoma, or multicentric Castleman's disease were not detected in the patient. Chemotherapy was effective and reduced the size of the lymphoma dramatically. This is the first case report of a germinal center B cell-originating lymphoma with HHV-8 infection. 相似文献
92.
Hamaguchi T Matsumura Y Nakanishi Y Muro K Yamada Y Shimada Y Shirao K Niki H Hosokawa S Tagawa T Kakizoe T 《Cancer science》2004,95(7):608-613
MCC-465 is an immunoliposome-encapsulated doxorubicin. The liposome is tagged with polyethylene glycol and the F(ab')2 of a monoclonal antibody named GAH, a human antibody obtained by the hybridoma technique. The epitope recognized by GAH is not well characterized, but human gastric, colorectal, and mammary cancer cells were GAH-positive, while the normal counterparts were GAH-negative. Pegylated liposome doxorubicin (PLD) and MCC-465 did not show significant antitumor activity against GAH-negative Caco-2 xenografts. On the other hand, MCC-465 exhibited significantly superior antitumor effects against GAH-positive WiDr-Tc and SW837 xenografts, compared with PLD. Immunohistochemistry with GAH revealed that 94% (100 of 106) of surgical specimens of colorectal cancer were GAH-positive. These results warrant a phase I clinical trial of MCC-465 for patients with metastatic colorectal cancer. 相似文献
93.
Wang J Kobayashi M Han M Choi S Takano M Hashino S Tanaka J Kondoh T Kawamura K Hosokawa M 《British journal of haematology》2002,118(2):638-645
Taxol is an effective anti-tumour drug against a variety of tumour cells. Taxol directly induces apoptosis in addition to a G2/M cell cycle arrest. However, it remains poorly understood how Taxol induces apoptosis in tumour cells. Taxol induces the secretion of inflammatory cytokines in murine macrophages in a toll-like receptor-4 (TLR-4)-dependent manner in addition to its anti-tumour effects, but the effect of Taxol on human macrophages is controversial. In this study, we demonstrated that low doses (less than 1000 nmol/l) of Taxol induced the expression of tumour necrosis factor (TNF)-alpha in human myelomonocytic cells and that the induction of TNF-alpha mRNA was inhibited by dominant-negative myeloid differentiation protein (dnMyD88). Furthermore, we demonstrated that the same doses of Taxol induced apoptosis of the same myelomonocytic cells and that the Taxol-induced apoptosis was also inhibited by dnMyD88. In accordance with the previous reports, Taxol induced the expression of TNF-alpha and apoptosis in a TLR4-independent manner. These results suggest that TNF-alpha expression and apoptosis, both induced by Taxol in human myelomonocytic cells, share the signal transduction molecule MyD88. 相似文献
94.
95.
96.
Inaba H Ohta S Yoshida H Oishi H Etoh T Honda A Nakajima N Muro H 《Kyobu geka. The Japanese journal of thoracic surgery》2003,56(5):347-52; discussion 353-5
After greater than 60 gray (Gy) irradiation, we performed the pulmonary resection in the 18 primary lung cancer cases. The mean irradiation dose to the tumor was 68.2 (range 60-101) Gy, and the mean irradiation dose to the bronchial stump was 47.1 (range 0-82) Gy. Median time from end of irradiation to surgical resection was 136 (range 20-894) days. One partial resection, 9 single lobectomies, 3 double lobectomies, and 5 pneumonectomies were done. Mainly, we closed the bronchial stump by the automatic stapling device and additional hand suturing. The bronchial stump was covered in the 12 cases by the owner stalk thymus, the intercostals muscular flap, the omentum flap, and et al. The major postoperative complications due to preoperative irradiation were not seen. Bronchopleural fistulas did not occur. Pathologically, the wall of the submucosal capillary vessels were getting thick in the patients operated more than 3 months later after irradiation. In such cases with the decrease of the blood flow, the bronchial stump should be covered. The pulmonary resection after the high dose irradiation was considered to be tolerable. 相似文献
97.
Takahashi T Fujisaki M Hirahata S Maeda D Kamei S Tokura H Inuzuka K Hunabashi M Hino T Ishida O 《Gan to kagaku ryoho. Cancer & chemotherapy》2003,30(7):993-997
We treated five cases for multiple hepatic metastases from gastric cancer with a novel combination of TS-1 and low-dose cisplatinum (CDDP). TS-1 was orally administered at 100 mg/body/day every day or only on weekdays, and 10 mg of CDDP was infused once or twice a week. The efficacy was evaluated by body CT after the treatment. The CT showed more than a 60% reduction of hepatic tumors in four patients. The tumor markers, CEA and CA19-9, were reduced to 10%. The response rate was 80%. Adverse reactions of grade 1 anemia were observed in two patients and grade 1 leucopenia in one patient. The liver function normalized in one patient. The hemoglobin level was increased from 6.8 g/dl to 11.8 g/dl in one patient. In conclusion, this combined chemotherapy of TS-1 and low-dose CDDP proved useful for advanced gastric cancer patients with multiple hepatic metastases, in view of its therapeutic efficacy, patients' quality of life and low toxicity. 相似文献
98.
Lung resistance-related protein as a predictor of clinical outcome in advanced testicular germ-cell tumours 总被引:14,自引:0,他引:14
Zurita AJ Diestra JE Condom E García Del Muro X Scheffer GL Scheper RJ Pérez J Germà-Lluch JR Izquierdo MA 《British journal of cancer》2003,88(6):879-886
This study was undertaken to investigate the expression and predictive value for outcome of multidrug resistance-associated (MDR) proteins P-glycoprotein (Pgp), MRP1, BCRP, and LRP, in advanced testicular germ-cell tumours (TGCT). Paraffin-embedded sections from 56 previously untreated patients with metastatic TGCT were immunostained for Pgp, MRP1, BCRP, and LRP. All patients received platinum-based chemotherapy after orchidectomy. Immunostaining was related to clinicopathological parameters, response to chemotherapy, and outcome. Strong and intermediate expressions of the different MDR-related proteins were: 27 and 41% (Pgp), 54 and 37% (MRP1), 86 and 7% (BCRP), and 14 and 29% (LRP). P-glycoprotein and MRP1 associated, respectively, to low AFP (P=0.026) and high LDH levels (P=0.014), whereas LRP expression associated with high beta-hCG levels (P=0.003) and stage IV tumours (P=0.029). No correlation was found between Pgp, MRP1, and BCRP expression and response to chemotherapy and survival. In contrast, patients with LRP-positive tumours (strong or intermediate expression) had shorter progression-free (P=0.0006) and overall survival (P=0.0116) than LRP-negative patients, even after individual log-rank adjustments by statistically associated variables. Our data suggest that a positive LRP immunostaining at the time of diagnosis in metastatic TGCT is associated with an adverse clinical outcome. 相似文献
99.
Matsushita K Kuramitsu Y Ohiro Y Kobayashi I Watanabe T Kobayashi M Hosokawa M 《International journal of oncology》2003,23(2):489-493
We have reported that ONO-4007, a novel synthetic lipid A derivative with low toxic activities, produced large amount of tumor necrosis factor (TNF)-alpha selectively in the tumor tissues and brought about complete cures in about 60% of rats bearing TNF-alpha sensitive KDH-8 cells, which also secreted a large amount of transforming growth factor (TGF)-beta. In our present study, to explore ONO-4007 induced Th1-type immune response, we investigated the mRNA expression of interferon (IFN)-gamma, Interleukin (IL)-1beta, IL-6, IL-10, IL-12 in KDH-8 bearing rats. We next examined the nitric oxide (NO) production. We found that IFN-gamma, IL-1beta, IL-6 and IL-12 mRNA expression of the tumor tissue were higher in the ONO-4007 treated rats than in phosphate buffer saline (PBS) treated rats. Western blotting also revealed that IL-12 protein production was increased. NO production from peritoneal macrophages were suppressed in tumor-bearing rats, but ONO-4007 restored it up to the normal level. These results suggest that ONO-4007 induces and restores Th1-type immune response through cytokine production cascade, followed by initial TNF-alpha production, eventually leading to tumor eradication. 相似文献
100.