全文获取类型
收费全文 | 9243篇 |
免费 | 459篇 |
国内免费 | 91篇 |
专业分类
耳鼻咽喉 | 58篇 |
儿科学 | 200篇 |
妇产科学 | 98篇 |
基础医学 | 1140篇 |
口腔科学 | 167篇 |
临床医学 | 499篇 |
内科学 | 2466篇 |
皮肤病学 | 108篇 |
神经病学 | 723篇 |
特种医学 | 299篇 |
外科学 | 1776篇 |
综合类 | 24篇 |
预防医学 | 174篇 |
眼科学 | 231篇 |
药学 | 642篇 |
中国医学 | 50篇 |
肿瘤学 | 1138篇 |
出版年
2023年 | 53篇 |
2022年 | 106篇 |
2021年 | 171篇 |
2020年 | 85篇 |
2019年 | 126篇 |
2018年 | 184篇 |
2017年 | 159篇 |
2016年 | 188篇 |
2015年 | 190篇 |
2014年 | 248篇 |
2013年 | 282篇 |
2012年 | 513篇 |
2011年 | 601篇 |
2010年 | 398篇 |
2009年 | 317篇 |
2008年 | 572篇 |
2007年 | 592篇 |
2006年 | 610篇 |
2005年 | 629篇 |
2004年 | 567篇 |
2003年 | 607篇 |
2002年 | 661篇 |
2001年 | 144篇 |
2000年 | 126篇 |
1999年 | 137篇 |
1998年 | 170篇 |
1997年 | 127篇 |
1996年 | 122篇 |
1995年 | 104篇 |
1994年 | 96篇 |
1993年 | 114篇 |
1992年 | 87篇 |
1991年 | 88篇 |
1990年 | 66篇 |
1989年 | 62篇 |
1988年 | 68篇 |
1987年 | 75篇 |
1986年 | 64篇 |
1985年 | 42篇 |
1984年 | 37篇 |
1983年 | 30篇 |
1982年 | 18篇 |
1981年 | 17篇 |
1980年 | 13篇 |
1979年 | 18篇 |
1977年 | 15篇 |
1975年 | 12篇 |
1974年 | 8篇 |
1973年 | 11篇 |
1970年 | 8篇 |
排序方式: 共有9793条查询结果,搜索用时 12 毫秒
91.
Thymidylate synthase (TS) is a rate-limiting enzyme in de novo DNA biosynthesis and also a primary target for 5-fluorouracil (5-FU)-based chemotherapy. To estimate the con-elation between the expression of TS in the tumor, clinical response and prognosis in cancer patients treated with 5-FU, we have prepared recombinant human TS (rhTS) protein by culturing of E. coli transfected with the plasmid (pGEX-2TH) encoding the nucleotide sequence of hTScDNA and then obtained a highly purified polyclonal antibody against rhTS protein. Using this anti-TS antibody, it was revealed that the content of TS protein, as determined by Western blot analysis, correlated with the enzyme activity (gamma=0.973) and cytotoxicity of 5-FU, expressed as IC50 value (gamma=0.954) against human colon tumor cells, sensitive to and with acquired-resistance to 5-fluoropyrimidines and other cancer cells. On immunochemical evaluation, it was also confirmed that the tumor cells overexpressing TS proteins were strongly stained by the polyclonal antibody when compared to the cell lines expressing TS to lower extent both in vitro and in vivo conditions. These results indicate that this purified polyclonal antibody to rhTS is applicable to prospective and retrospective clinical studies on immunochemical TS expression in various tumors as a prognostic factor and 5-FU response-predicting parameter. 相似文献
92.
Tomosaburo Sakamoto Masahiko Miyata Makoto Nakamuro Masaaki Izukura Wataru Kamiike Hikaru Matsuda 《Surgery today》1994,24(12):1078-1080
A 51-year-old Japanese man who underwent a standard distal gastrectomy for cancer of the stomach developed abdominal pain when oral intake was commenced on the 6th postoperative day after an uneventful postoperative course. Complete obstruction of the jejunum led to a sudden deterioration in his general condition and a laparotomy was performed, revealing counterclockwise rotation of the mesenterium. The necrotic portion of the small intestine was removed, while 10 cm of the upper jejunum and 100 cm of the terminal ileum were preserved. His second postoperative course was uneventful apart from the development of intestinal hurry, which is now under medical control 9 months after his second laparotomy. 相似文献
93.
The anti-proliferative effect of proliferating cell nuclear antigen-specific antisense oligonucleotides on human gastric cancer cell lines 总被引:4,自引:0,他引:4
Chouhei Sakakura Akeo Hagiwara Hiroyuki Tsujimoto Kimihiko Ozaki Tsuguo Sakakibara Takayuki Oyama Masaharu Ogaki Toshio Takahashi 《Surgery today》1995,25(2):184-186
The proliferating cell nuclear antigen (PCNA) is a nuclear protein that leads DNA synthesis by the DNA polymerase delta. As the PCNA gene is strongly expressed in invasive gastric cancer cells with high proliferative activity, PCNA is suspected of playing an important role in the proliferation and advancement of gastric cancer. Thus, the effects of antisense oligonucleotides specific for PCNA mRNA were examined in seven gastric cancer cell lines. It was found that treatment with antisense oligonucleotides at concentrations of 10–40 M dose-dependently inhibited the growth of all cell lines; however, random sequence oligonucleotides did not modify the proliferation of any type of cells. These results indicate that PCNA is essential for cell proliferation in gastric cancer cells, and that the growth inhibitory effect results from the inhibition of PCNA gene expression. Therefore, PCNA-specific antisense oligonucleotides may be effective in the treatment of gastric cancer. 相似文献
94.
Tsujimoto Y Oka T Noguchi T Fujii T Miyagawa Y Takano Y Yasunaga Y Takaha M Kanno N 《Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology》1999,90(12):920-923
A 32-year-old man consulted Osaka National hospital with chief complaints of dysuria and macrohematuria. DIP and CT revealed that the right kidney deviated to the lower pole of the left kidney and they fused together. The right ureter crossed over the supine. The calcified shadow existed in the lower end of the left ureter with cobra head image. He had no external anomalies. Under diagnosing crossed fused kidney (inverted L shaped) complicated the left ureterocele with a stone, transurethral incision of ureterocele (TUI) was performed. We made transverse incision and extracted stone, 7 mm in size (calcium oxalate 96% and calcium phosphate 4%). Three months later after the operation, IVP, CG and VCG revealed the down-sized ureterocele and no VUR. Crossed renal ectopia complicated many anomalies about 50%. Among them anomalies of the urinary tract was most frequent about 30%. But crossed renal ectopia with ureterocele wasn't reported so far in Japanese literature. 相似文献
95.
Tamakawa S Tsujimoto J Iharada A Ogawa H 《Masui. The Japanese journal of anesthesiology》1999,48(3):292-294
The purpose of this study is to find the most effective concentration of topical prostaglandin E1 (PGE1) to treat pain in postherpetic neuralgia (PHN). The concentrations of PGE1 dissolved in vaseline were 0 microgram.g-1, 2 micrograms.g-1, 4 micrograms.g-1 and 8 micrograms.g-1. Visual analog scale (VAS) score was reduced after the ointment application in relation to the concentration of PGE1 with initial relief at 25 minutes and lasting for 5 hours. Three patients medicated with PGE1 8 micrograms.g-1 and one patient medicated with 4 micrograms.g-1 complained of topical pain of the skin. Topical PGE1 dissolved in vaseline is an effective means of reducing pain due to PHN. Probably 4 micrograms.g-1 of the ointment is most useful to treat PHN. 相似文献
96.
T. Goto Masahiko Shimura Mitsuru Nakazawa 《Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie》1999,237(9):787-789
· Background: To investigate the usefulness of indocyanine green (ICG) iris angiography for monitoring vascular abnormalities
and the clinical course of metastatic iris tumor during chemotherapy. · Methods: We performed ICG iris angiography at several
points during systemic chemotherapy for a 67-year-old man who had been diagnosed as having small-cell carcinoma of the lung
with metastatic iris tumors. · Results: ICG iris angiography clearly demonstrated hyperfluorescent tumor vessels, rubeosis
iridis, and dilated iris stromal vessels. After chemotherapy, these hyperfluorescent vessels and rubeosis regressed. · Conclusion:
ICG iris angiography appears to be an effective and useful method for observing abnormal vessels associated with metastatic
iris tumors.
Received: 11 May 1998 Revised version received: 30 July 1998 Accepted: 13 August 1998 相似文献
97.
98.
The structural organization and 5'-flanking region of the mouse V1a and V1b vasopressin receptor genes were determined. The mouse V1a receptor gene was located within an 8-kb XbaI fragment, and the mouse V1b receptor gene was located within a 14-kb EcoRV fragment. Both genes were comprised of two coding exons that were separated by a 2.3-kb and a 8.0-kb intron, respectively, located before the respective seventh transmembrane domain of the receptor sequence. The availability of these genes would allow us to study the functional role of V1a and V1b receptors by disrupting the gene in mice. 相似文献
99.
Shiraishi M Suzuki K Abe T Kikuchi T Satoh H Nakaji S Sugawara K 《Environmental health and preventive medicine》1996,1(2):65-70
Neutrophil functions, including chemotaxis, reactive oxygen species (ROS)-producing capacity of neutrophils, and serum opsonic activity were investigated in 9 young healthy male volunteers. Venous blood of these volunteers was obtained under standardized conditions at 4-h intervals over a 24-h span. Neutrophil chemotaxis was evaluated by a modified Boyden technique, ROS-producing capacity of neutrophils and serum opsonic activity were measured by a simultaneous multiple measurement system based on luminol-dependent chemiluminescence and indicated by peak height and peak time. ROS-producing capacity of neutrophils and serum opsonic activity were activated in the daytime, and decreased from night to morning. There were negative correlations between the peak time of the luminol-dependent chemiluminescent response, neutrophil number (p<0.01) and segmented neutrophil number (p>0.01). On the other hand, no significant correlations were noted between serum opsonic activity and IgG, IgA, IgM, C3 or C4. In contrast, the peaks of neutrophil chemotaxis were at the wake-up time (6:00a.m.) and in the evening (6:00p.m.). This study indicates that diurnal variation of neutrophil function exists. 相似文献
100.
Naohiro Kajiwara Masahiko Taguchi Hiroshi Saito Shin Nakajima Aeru Hayashi Norihiko Kawate Chimori Konaka Hiromi Wada Harubumi Kato 《Transplant international》1996,9(3):194-200
We examined the efficacy of two new preservation solutions containing trehalose-an extracellular type (ET-K) of solution and an intracellular type (IT-K) of solution — in relation to that of Euro-Collins (EC) solution in 20-h canine lung preservation. Canine lungs were flushed with one of the three solutions (n=5 for each solution) after pretreatment with PGE1 (20 g/kg) and were stored for 20 h at 4°C. The left lungs were transplanted and evaluated to 6 h post transplant. In the ET-K group, the arterial oxygen tension after reperfusion was significantly higher than in the IT-K and EC groups. The pulmonary vascular resistance, wet/dry weight ratio, and histological evaluation of each transplanted lung in the ET-K group were also better than in the IT-K and EC groups. This indicates that ET-K solution is useful for 20-h preservation of canine lung grafts. 相似文献