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41.
Andre V. Bochkov Marcela Lareschi Mauricio Barreto 《Acta parasitologica / Witold Stefański Institute of Parasitology, Warszawa, Poland》2014,59(4):586-595
Six fur-mite species of the genus Prolistrophorus Fain, 1970 (Acariformes: Listrophoridae) were recorded from Central and South American rodents of the subfamily Sigmodontinae (Rodentia: Cricetidae). Among them, Prolistrophorus (Aprolistrophorus) parabidentatus sp. nov. from Akodon azarae from Argentina and Prolistrophorus (Aprolistrophorus) tylomys sp. nov. from Tylomys nudicaudus from Guatemala are described as new for science. New hosts are recorded for the following species: Prolistrophorus (Prolistrophorus) grassii (Radford, 1954) from Zygodontomys brevicauda from Colombia, P. (P.) frontalis (Hirst, 1921) from Oligoryzomys sp. from Argentina, P. (P.) argentinus (Hirst, 1921) from Melanomys caliginosus, Akodon affinis from Colombia and Scapteromys aquaticus from Argentina, Prolistrophorus (Beprolistrophorus) hirstianus Fain, 1973 from Scapteromys aquaticus from Argentina. 相似文献
42.
Elisabete Andrade Daniele Rocha Marcela Fontana-Maurell Elaine Costa Marisa Ribeiro Daniela Tupy de Godoy Antonio G.P. Ferreira Amilcar Tanuri Patrícia Alvarez Rodrigo Brindeiro 《The Brazilian journal of infectious diseases》2018,22(5)
The Brazilian Public Health Service provides freely αPEG-IFN to treat patients infected with HCV. The primary goal of HCV therapy is the long-term elimination of HCV from the blood to reduce the risk of HCV associated complications and death. Patient viremia affects the treatment duration and response, thus influencing clinical decisions. We developed a high-throughput method to perform the quantification of RNA hepatitis C virus (HCV) virus load in plasma samples to monitor patients under treatment. The method is based on a duplex detection, in a one-step real-time RT-PCR assay and it has been validated according to the rules established by the official Brazilian regulatory agency (ANVISA). This new method was compared to a commercial kit (Cobas/Taqman HCV Test v2.0 - Roche), showing virus load results with significant correlation between them (p?=?0,012) using commercial and clinical panels. In addition, 611 samples from patients treated with peguilated alfa-interferon (αPEG-IFN) from different regions of Brazil were analyzed. Our one-step real-time RT-PCR assay demonstrated good performance in viral load measurement and in treatment course monitoring, with acceptable sensitivity and specificity values 相似文献
43.
Marcela Durán Alexandre N. Ponezi Adelaide Faljoni-Alario Maria F. S. Teixeira Giselle Z. Justo Nelson Durán 《Medicinal chemistry research》2012,21(7):1524-1532
Violacein is a versatile pigment from a bacterium Chromobacterium violaceum that exhibits several biological activities and, at present, has gained increasing importance in industrial markets, such as in medicine, cosmetics, and textiles. In this mini-review, we aimed to describe violacein production and to explore its various biological properties in a pharmacological context, including its antioxidant, immunomodulatory, antitumoral, and antiparasitic activities. In addition, its use in the fields of cosmetics, textiles, food, toys, and insecticides has emerged as unusual potential areas of application to be discussed here. 相似文献
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45.
Frederico Figueiredo Amancio Maira Alves Pereira Felipe Campos de Melo Iani Lorena D'anuncia??o Jorge Luís Carvalho de Almeida Janer Aparecida Silveira Soares Marcela Lencine Ferraz Thiago Cardoso Vale José Roberto Lambertucci Mariangela Carneiro 《Revista do Instituto de Medicina Tropical de S?o Paulo》2014,56(3):267-270
Dengue is currently a major public-health problem. Dengue virus (DENV) is
classified into four distinct serotypes, DENV 1-4. After 28 years of absence, DENV-4
was again detected in Brazil in 2010 in Roraima State, and one year later, the virus
was identified in the northern Brazilian states of Amazonas and Pará, followed by Rio
de Janeiro and São Paulo. In Minas Gerais, the first confirmed case of DENV-4
occurred in the municipality of Frutal in 2011 and has now been isolated from a
growing number of patients. Although DENV-2 is associated with the highest risk of
severe forms of the disease and death due to the infection, DENV-4 has also been
associated with severe forms of the disease and an increasing risk of hemorrhagic
manifestations. Herein, the first fatal case of confirmed DENV-4 in Brazil is
reported. The patient was an 11-year-old girl from the municipality of Montes Claros
in northern Minas Gerais State, Brazil. She had idiopathic thrombocytopenic purpura
as a comorbid condition and presented with a fulminant course of infection, leading
to death due to hemorrhagic complications. Diagnosis was confirmed by detection of
Dengue-specific antibodies using IgM capture enzyme-linked immunosorbent assay and
semi-nested RT-PCR. Primary care physicians and other health-care
providers should bear in mind that DENV-4 can also result in severe forms of the
disease and lead to hemorrhagic complications and death, mainly when dengue infection
is associated with coexisting conditions. 相似文献
46.
47.
Andrea Gadano Alberto Gurni Marcela Nigro López Paula López Adriana Gratti Catalina van Baren 《Pharmaceutical biology》2013,51(1):7-12
The cytogenetic effects of aqueous extracts of Chenopodium multifidum L. (Paico) were determined by addition of the extracts and fractions to human lymphocyte cultures. Toxicity was evaluated by analysis of chromosomal aberrations (CA), sister chromatid exchange (SCE), mitotic (MI) and replication (RI) indexes. The results showed an increase in CA frequency in cultures exposed to infusion decoction, no modification in the CPK values either in the decoction or in the infusion, and a decrease in the MI of lymphocyte cultures exposed to the decoction. These results suggested genotoxic effects of “Paico” aqueous extracts. 相似文献
48.
49.
Marcela da Silva Rubio Adriana Maria de Almeida Angelo Berchieri Junior 《Avian pathology》2017,46(6):644-651
Currently there are 2659 Salmonella serovars. The host-specific biovars Salmonella Pullorum and Salmonella Gallinarum cause systemic infections in food-producing and wild birds. Fast diagnosis is crucial to control the dissemination in avian environments. The present work describes the development of a multiplex qPCR in real time using a low-cost DNA dye (SYBr Green) to identify and quantify these biovars. Primers were chosen based on genomic regions of difference (RoD) and optimized to control dimers. Primers pSGP detect both host-specific biovars but not other serovars and pSG and pSP differentiate biovars. Three amplicons showed different melting temperatures (Tm), allowing differentiation. The pSGP amplicon (97?bp) showed Tm of 78°C for both biovars. The pSG amplicon (273?bp) showed a Tm of 86.2°C for S. Gallinarum and pSP amplicon (260?bp) dissociated at 84.8°C for S. Pullorum identification. The multiplex qPCR in real time showed high sensitivity and was capable of quantifying 108–101 CFU of these biovars. 相似文献
50.