Fluorescence and Laser Photon Counting: Measurements of Epithelial [Ca2+]i or [Na+]i with Ciliary Beat Frequency

We describe a system we developed that enabled simultaneous measurements of either epithelial calcium ion concentration ([Ca²⁺]i) or sodium ion concentration [Na⁺]i with the ciliary beat frequency (CBF) in native ciliated epithelia using either Fura-2 (AM) or SBFI (AM) ratiometric fluorescence photon counting along with nonstationary laser light scattering. Studies were performed using native epithelial tissues obtained from ovine tracheae. The dynamic range of the laser light-scattering system was determined by a simulated light beating experiment. The nonstationary CBF was demonstrated by the time-frequency analysis of the raw photon count sequences of backscattered heterodyne photons from cultured and native epithelia. Calibrations of calcium and sodium ion concentrations were performed using the respective Fura-2 and SBFI impermanent salts as well as in native epithelia. The cumulative responses of 10^–6, 10^–5, and 10^–4} M nifedipine on [Ca²⁺]i together with the CBF as well as the cumulative responses of 10^–5, 10^–4, and 10^–3 M amiloride on [Na⁺]i together with the CBF were also determined. Nifedipine decreased [Ca²⁺]i but had no effect on CBF. Amiloride decreased [Na⁺]i and CBF. Stimulation of CBF corresponded with either an increase of [Na⁺]i or an increase of [Ca²⁺]i. Decreases of [Na⁺]i or substantial decreases of [Ca²⁺]i were associated with decreases in the CBF. These data demonstrate the utility of this system for investigating the regulatory mechanisms of intracellular ions dynamics and the CBF in native epithelia. © 1998 Biomedical Engineering Society. PAC98: 8780+s, 8722-q, 4262Be     

SLE患者淋巴细胞膜上IL-2R表达的动态分析

本实验采用Wu Tac单克隆抗体的间接荧光抗体法,动态观察了系统性红斑狼疮(SLE)患者和正常供血员(对照组)的外周血淋巴细胞经PHA刺激后,于不同时间(0、24、48、72小时)内细胞膜上白细胞介素2受体(IL-2R)的表达。结果表明在给予PHA刺激培养24、48和72小时后,与对照组比较SLE患者淋巴细胞的IL-2R表达明显下降,提示了SLE的T淋巴细胞反应性降低可能与被激活的T细胞表面IL-2R的表达功能缺陷有关。此结果将有助于进一步探讨SLE的免疫调节紊乱的发生机制。     

微波体模

用水、盐,聚氯乙烯粉和羧甲基纤维钠四种材料配制成一种适用的微波体模(模拟肌肉组织)。该体模的复介电系数在300～2500MHz范围内与实际人体肌肉组织的误差≤5％。这种体模的优点是材料立足国内,价廉,室温下配制,使用方便。本文也给出这种体模的温度特性,稳定性和热学特性,供电磁场生物效应和微波热疗等研究使用。     

偶联剂CGE（N）—R型处理前后牙本质的表面状态及其...

In order to research the states of dentine surface and dentine surface primed with coupling agent, we used microscope, transmission electron microscope (TEM) and solid fluorescence method. Observed under microscope, patterning of dentinal tubules and their poles was very explicit; through the view of interface between coupling agent adsorbed and human dentine powder by TEM, it was revealed that the coupling agent was adsorbed onto the dentine surface as if it "grew out" of the dentine surface; through examination of interface between the coupling agent adsorbed and dentine surface by fluorescence spectrum, which differed from that of the coupling agent or dentine surface, i.e., this fluorescence spectrum of interface changed obviously (red shift). This phenomenon indicated that there was a chemical reaction occurring between tooth surface and adsorbed coupling agent. Therefore we can conclude that the three examinations are necessary to provide the argument for establishment of chemical bonding mechanism between dentine and coupling agent.     

Primary study of iontophoresis of zinc ion in treatment of retinitis pigmentosa

We report here the preliminary effects of zinc iontophoresis in treatment of 26 Rp patients who had decreased zinc serum level or abnormal ratio of serum copper to zinc. Through more than 20 to 30 times of therapy, the vision of 15.38% eyes increased more than "3 lines", 4% eyes increased more than 5 degrees in the visual field, 13.46% eyes improved in dark adaptation threshold (greater than 1.0), the visual functions of the rest eyes were slightly improved or unimproved. The fundus didn't change before & after treatment. There were no changes of ERG in 8 eyes of 4 cases. In addition, the advantages and disadvantages between Zn iontophoresis and Zn compound taken orally, the improvement of vision by direct electric current therapy, the relationship between the metabolism of Zn element and Vit A and influence of Zn over the mechanism of dark adaptation were also briefly discussed.     

Management of hemangiopericytomas in the central nervous system

目的　血管外皮细胞瘤是少见的中枢神经系统肿瘤 ,由于其易复发和转移而预后不良。本文介绍 2 6例血管外皮细胞瘤的处理经验并结合文献复习其诊治要点。方法　回顾性分析 1993年 1月至 1999年 6月华山医院收治的 2 6例血管外皮细胞瘤 ,其中 5例为复发肿瘤 ,1例为术后残留。结果　 2 6例中 2 4例病灶全切除 ,2例次全切除。术后 2 1例症状好转或不变 ,4例加重 ,1例手术后死于并发症。18例术后放疗。 18例获随访 ,随访期 1- 5 8个月 ,其中 15例恢复良好 ,2例发现转移肿瘤 ,1例复发再次手术后死亡。结论　手术全切是主要治疗方法 ,术后放疗可延缓肿瘤复发。     

驻极体诱导3T3细胞内游离钙离子浓度的变化

目的：研究公主体对3T3细胞内游离Ca^2 浓度变化的,体诱导3T3细胞凋亡与游离Ca^2 浓度变化之间的关系。方法：选用－300V和－500V驻极体作用3T3细胞48h,即时依次加入CaCl2,A23187,EDTA,通过流式细胞术检测细胞内Ca^2 浓度的变化和3T3细胞的凋亡量。结果：（1）负极性驻极体作用3T3细胞后,3T3细胞出现10％的凋亡量;（2）驻极体作用3T3细胞以后,细胞质游离Ca^2 浓度升高,Ca^2 浓度的升高不是由于细胞内钙池的释放,而是由于细胞外Ca^2 内流所引起,结论：驻极体诱导3T3细胞凋亡的机制可能是细胞外Ca^2 内上起的。     

透析前注射单剂量低分子量肝素对透析器复用效率的评价

目的 评价血透前注射单剂量低分子量肝素（LMWH）与持续输注普通肝素（SH）对复用透析器效率的影响。方法 对30例慢性透析患者进行随机交叉对照研究,观察第1、4次透析的透析器纤维包裹容量（FBV）、透析前血球压积（HCT）及透析2h尿素氮、肌酐清除率;另外,利用生色底物法测定透析0h,2h,4h血浆肝素抗－Fxa水平。结果 同SH组比,LMWH组透析器复用次数增加（P＜0．05）,第4次复用透析器FBV及透析2h尿素氮、肌酐清除率无下降（P＞0．05）,SH组则下降（P＜0．05）,透析2h两组血浆肝素活性－Fxa水平差异无显著性（P＞0．05）,透析4hLWMH组高于SH组（P＜0．05）。结论 透析前单剂量注射LMWH能有效地保护复用透析器清除率,值得临床上进一步推广。     

小儿幽门螺杆菌感染与胃粘膜上皮细胞凋亡

目的　探讨幽门螺杆菌 (H .pylori)相关性小儿慢性胃炎胃粘膜上皮细胞凋亡和相关基因 Fas的变化 .方法　采用 TUNEL技术和 Envision免疫组化方法检测 19例 H .py-lori阴性慢性胃炎和 17例 H.pylori阳性慢性胃炎患儿 H.pylori清除前后胃粘膜上皮细胞凋亡和相关基因 Fas的变化 .结果　 19例 H.pylori阴性小儿胃炎胃粘膜上皮细胞凋亡指数和 Fas指数分别为 (2 .7± 0 .8) %和 (11.7± 9.0 ) % ;17例 H.pylori阳性小儿慢性胃炎胃粘膜凋亡指数和 Fas指数分别为 (8.5± 1.8) %和 (31.3± 1.9) % ;H.pylori阳性组凋亡指数和 Fas指数明显高于 H .pylori阴性组 P<0 .0 1. H .py-lori清除后胃粘膜上皮凋亡指数和 Fas指数均明显减少(P<0 .0 1) ,治疗前凋亡指数和 Fas指数分别为 (8.6± 2 .3) %和 (32 .6± 12 .7) % ;清除 H.pylori后 ,凋亡和 Fas指数分别为 (3.6± 1.8) %和 (13.3± 6 .4) % .结论　 H .pylori感染促进胃粘膜上皮细胞的凋亡 ;Fas基因过度表达可能是 H.py-lori诱导胃粘膜上皮细胞凋亡增加的机制之一 .     

昆明种系小鼠胚胎干细胞的分离培养及特性鉴定

目的 提高昆明小鼠胚胎干细胞（ES细胞）建株的成功率。方法 收集小鼠3．5d的囊胚培养,用小鼠胚胎成纤维细胞作为饲养层,形成的ES细胞样集落,经两次亚克隆分离培养ES细胞;进行相差显微镜观察、碱性磷酸酶染色和体内外分化能力鉴定。结果 获得两个稳定ES细胞集落,传至第11代。ES细胞呈集落样生长,碱性磷酸酶染色强阳性,体外分化的细胞沿拟胚体外向性生长,形态多样,在体分化可形成来源于3个胚层的组织。结论 两次亚克地获得的细胞具有ES细胞主要的生物学性状,有助于提高昆明小鼠ES细胞建株的能力。