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Zusammenfassung Die Konservierung von Spalthauttransplantaten bei 4°C stellt eine einfache Aufbewahrungsart dar, bei der die Haut bis zu 14
Tagen vital bleibt. Unsere klinischen Erfahrungen mit dieser Methode bei bisher 55 F?llen und die Vorteile der Anwendung von
Fremdhauttransplantaten in der plastischen Chirurgie werden dargelegt, diskutiert und an 2 klinischen Fallberichten demonstriert.
Summary There is a growing need for split skin homografts and heterografts in plastic surgery. The storage of split skin grafts at 4°C in a humid chamber is a simple and non expensive method of skin graft conservation. The grafts remain vital up to 2 weeks. Our clinical experiences with this method on 55 cases are reported. The qualities and the advantages of application of homografts and heterogrofts are discussed and demonstrated on two clinical cases.相似文献
44.
Margittai E Bánhegyi G Kiss A Nagy G Mandl J Schaff Z Csala M 《The Journal of nutrition》2005,135(11):2530-2534
Insufficient ascorbate intake causes scurvy in certain species. Beyond its known functions, it has been suggested that ascorbate participates in oxidative protein folding in the endoplasmic reticulum (ER). Because redox imbalance in this organelle might cause ER stress and apoptosis, we hypothesized that this might contribute to the pathology of scurvy. Guinea pigs were divided into 7 groups: the control group was fed a commercial guinea pig food containing 0.1 g/100 g ascorbate for 4 wk, 5 groups consumed an ascorbate-free food for 0, 1, 2, 3, or 4 wk and 1 group was fed this scorbutic diet for 2 wk and then the commercial food plus 1 g/L ascorbate in drinking water for 2 wk. TBARS generation and the expression of some ER chaperones and foldases were determined in hepatic microsomes. The apoptotic index was assessed in histological sections. Although ascorbate, measured by HPLC, was undetectable in the livers of the guinea pigs after they had consumed the scorbutic diet for 2 wk, the microsomal TBARS level was elevated relative to the initial value only at wk 4. Western blot revealed the induction of GRP78, GRP94, and protein disulfide isomerase at wk 3 and 4. Apoptosis was greater than in the control, beginning at wk 3. None of the alterations occurred in the groups fed the commercial guinea pig food or ascorbate-free food followed by ascorbate supplementation. Therefore, persistent ascorbate deficiency leads to ER stress, unfolded protein response, and apoptosis in the liver, suggesting that insufficient protein processing participates in the pathology of scurvy. 相似文献
45.
P. Wurnig H. Tilscher S. Rummelhardt H. Jenny P. Porges O. Mayrhofer W. Mandl Eva Mannheimer W. F. Pinggera 《European Surgery》1977,9(1):20-22
Ohne Zusammenfassung 相似文献
46.
Saletu MT Anderer P Saletu-Zyhlarz GM Mandl M Arnold O Nosiska D Zeitlhofer J Saletu B 《European archives of psychiatry and clinical neuroscience》2005,255(1):20-32
The aim of the present study was to investigate
the role of EEG mapping as an objective and quantitative
measure of vigilance in untreated and
modafinil–treated narcoleptics, and compare it with the
conventional neurophysiological method of the Multiple
Sleep Latency Test (MSLT) and the subjective Epworth
Sleepiness Scale (ESS). In 16 drug–free narcoleptics
and 16 normal controls a baseline 3–min
vigilance–controlled EEG (V–EEG) and a 4–min resting
EEG (R–EEG) were recorded during midmorning hours.
Thereafter, in a double–blind, placebo–controlled crossover
design, patients were treated with a 3–week fixed
titration of modafinil (200, 300, 400 mg) and placebo.
EEG–mapping, MSLT and ESS measures were obtained
before and at the end of the third week of therapy. Statistical
overall analysis by means of the omnibus significance
test demonstrated significant EEG differences between
untreated patients and controls in the resting
condition only (R–EEG). Subsequent univariate analysis
revealed an increase in absolute and relative theta
power, a decrease in alpha–2 and beta power as well as a
slowing of the dominant frequency and the centroids of
the alpha, beta and total power spectrum and thus objectified
a vigilance decrement in narcolepsy. Modafinil
400 mg/d significantly improved vigilance as compared
with placebo (p 0.01), inducing changes opposite to
the aforementioned baseline differences (key–lock principle).
The MSLT and the ESS also improved under
modafinil as compared with placebo, but changes were
less consistent. Spearman rank correlations revealed the
highest correlations between EEG mapping and the ESS,
followed by those between EEG mapping and the MSLT,
while the lowest correlation was found between the
MSLT and the ESS. In conclusion, EEG mapping is a valuable
instrument for measuring vigilance decrements in
narcolepsy and their improvement under psychostimulant
treatment. 相似文献
47.
Decreased thalamic blood flow in obsessive-compulsive disorder patients responding to fluvoxamine 总被引:1,自引:0,他引:1
Ho Pian KL van Megen HJ Ramsey NF Mandl R van Rijk PP Wynne HJ Westenberg HG 《Psychiatry research》2005,138(2):89-97
Functional imaging studies have pointed to a role of the orbitofrontal cortex (OFC), striatum and thalamus in the pathophysiology of obsessive-compulsive disorder (OCD). Effective treatment has been found to change brain activity within this circuitry. The aim of the present study was to explore possible differential effects of OCD responders and non-responders to drug treatment on the regional cerebral blood flow (rCBF). Measurements of rCBF were carried out in 15 out of 22 patients with OCD who completed an open-label trial with fluvoxamine. Patients were studied with 99mTc-HMPAO single photon emission computed tomography (SPECT) before and after 12 weeks of treatment. In addition, structural magnetic resonance imaging was obtained on all patients. Regions of interest comprised the OFC, caudate nucleus, putamen and thalamus. Seven patients responded to treatment. Levels of rCBF decreased significantly in the left caudate nucleus and the left and right putamen in both responders and non-responders to treatment. In responders, but not in non-responders, a significant decrease in rCBF was found in the right thalamus. Pre-treatment cerebellar and whole brain HMPAO uptake was significantly higher in responders to treatment compared with non-responders. We suggest that the thalamus plays a central role in the response to drug treatment. 相似文献
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For tissue engineering of autologous cartilage, cell expansion is needed to obtain the cell numbers required. Standard expansion media contain bovine serum. This has several disadvantages, that is, the risk of transmitting diseases and serum-batch variations. The aim of this study was to find a serum-free medium with at least the same potential to expand cell numbers as serum-containing media. Ear chondrocytes of three young children were expanded in either serum-containing medium (SCM; DMEM with 10% fetal calf serum) or serum-free medium (SFM; DMEM with ITS+) supplemented with 5 or 100 ng/mL fibroblast growth factor-2 (FGF2). To promote cell adherence onto the culture flask, the serum-free conditions were cultured with 10% serum for 1 day after each trypsinization. After the fourth passage, the chondrocytes were encapsuled in alginate beads and redifferentiated in a SFM (DMEM with ITS+, hydrocortisone, and L-ascorbic acid) supplemented with 10 ng/mL IGF-I and 10 ng/mL TGFbeta-2. Results showed that expansion in SFM with 100 ng/mL FGF2 was comparable to expansion in SCM. Redifferentiation with SFM with IGF-I and TGFbeta-2 showed high collagen type II expression and high GAG/DNA production regardless of which expansion medium had been used. However, chondrocytes expanded in SFM with 100 ng/mL FGF2 resulted in less positive cells for collagen type I and 11-fibrau (a fibroblast membrane marker). The present study shows that it is possible to use serum-free medium for tissue engineering of cartilage. Expansion of immature ear chondrocytes in SFM supplemented with high-concentration FGF2 resulted in high cell numbers, which in addition had better redifferentiation capacity than cells expanded in medium with 10% serum. 相似文献