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排序方式: 共有163条查询结果,搜索用时 15 毫秒
101.
Soundappan SV Soundappan Jonathan Karpelowsky Albert Lam Lawrence Lam Danny Cass 《Journal of pediatric surgery》2018,53(10):2023-2027
Aim
Compare the diagnostic accuracy of surgeon performed ultrasound to radiology performed ultrasound in children presenting with suspected appendicitis to a tertiary care pediatric hospital in Australia.Methods
Children under 16 presenting to the emergency department of The Children's Hospital at Westmead were considered for the study. Patients with obvious signs of appendicitis not requiring ultrasound and those with established ultrasound diagnosis of appendicitis were excluded. Ultrasound was performed by a Pediatric Surgeon (SPU) after obtaining consent. The treating team was blinded to the results. Patient underwent formal ultrasound in radiology (RPU) and treatment was based on the formal report. SPU result was reviewed by a radiologist blinded to results of RPU. The results were compared.Results
65 children underwent ultrasound. 35 were male. Median age was 10 (range3–15). Median weight was 36 kg (range 12.6–76.2 kg), z-score median 0.21 (? 1.83 to 2.74). Symptom duration ranged from few hours to 2?weeks but majority (45) had symptoms for less than 48?h. Prevalence of appendicitis was 45%. Thirty two underwent surgery. Negative appendicectomy rate was 9.4%. Thirty three did not have surgery. 8 represented but only one proceeded to appendicectomy. SPU was done earlier than RPU (median 12?h vs 14.15?h) p?=?0.088. Diagnostic accuracy using ROC did not reveal significant difference.Conclusion
SPU can be performed earlier than RPU with reliable accuracy. Training surgical trainees will enable early diagnosis and management of appendicitis. 相似文献102.
MS Jacobson ; SV Kevy ; GM Thorne ; DA Goldmann ; L Blasetti ; JW Smith ; H Schaefer 《Transfusion》1990,30(2):146-149
The ability to store platelets beyond 24 hours requires a functionally closed system. This study tested the ability of a cell separator bowl seal system to resist penetration of microbial contamination under normal running conditions and under extreme environmental stress. Three test organisms, Micrococcus luteus, Serratia marcescens, and Staphylococcus epidermidis, were applied directly to the bowl at the edge of the seal or aerosolized and passed through the centrifuge chamber while the cell separator was run through a simulated platelet collection. A sterile, bacteriologic nutrient medium was perfused through the tubing set, thus simulating the flow of blood fractions. Following the procedure, the medium was examined for microbial growth. The concentration of aerosolized bacteria ranged from 5.2 x 10(1) to 3.9 x 10(3) colony-forming units (CFU) per mL, and the concentration of bacteria applied to the edge of the seal ranged from 1.9 x 10(5) to 2.8 x 10(9) CFU per mL. The positive control, direct inoculation of S. marcescens into the circulating medium (50 CFU/500 mL), resulted in recovery of the identical organism after 24 hours' incubation. No contamination of the system was detected in 40 experiments with aerosolized bacteria or in 32 experiments in which bacteria were applied directly to the seal. This study demonstrates that this sealed-bowl system resists microbial contamination. 相似文献
103.
高剂量地塞米松为多发性骨髓瘤常用治疗方案。本研究比较了高、低剂量地塞米松分别联合来那度胺治疗多发性骨髓瘤的临床疗效及毒副作用。 相似文献
104.
105.
106.
Impact of Helicobacter pylori eradication on morphological changes in gastric mucosa 总被引:2,自引:0,他引:2
Pimanov SI Makarenko EV Krylov IuV Matveenko ME Malashenko SV Bondarenko VM 《Arkhiv patologii》2006,68(5):22-27
The purpose of the study was to examine gastric mucosal morphological changes in patients with gastroduodenal pathology after eradication therapy for Helicobacter pylori (H. pylori). A hundred and thirty-eight patients (40 females and 98 males) were examined. Of them, there were 122 patients with duodenal peptic ulcer, 8 with gastric peptic ulcer, 5 with erosive gastritis, 2 with chronic atrophic antral gastritis, and 1 with non-atrophic gastritis. Two months and a year after therapy, manifestations of gastric mucosal atrophy, the degree of inflammation, and its activity significantly diminished in patients with complete H. pylori eradication. Positive changes were observed mainly in the antral portion of the stomach. In patients with partial eradication, chronic inflammation and its activity became less. Two months and a year following therapy, positive changes in the gastric mucosa were absent in patients without H. pylori eradication. 相似文献
107.
The roles of von Willebrand factor and factor VIII in arterial thrombosis: studies in canine von Willebrand disease and hemophilia A 总被引:2,自引:3,他引:2
Nichols TC; Bellinger DA; Reddick RL; Smith SV; Koch GG; Davis K; Sigman J; Brinkhous KM; Griggs TR; Read MS 《Blood》1993,81(10):2644-2651
We have studied the roles of von Willebrand factor (vWF) and factor VIII in arterial thrombosis in four canine phenotypes: normal (n = 6), hemophilia A (n = 11), von Willebrand disease (vWD) (n = 9), and hemophilia A/vWD (n = 1). vWF activity was determined by botrocetin- induced agglutination of fixed human platelets and vWF antigen (vWF:Ag) by Laurell electroimmunoassay and crossed immunoelectrophoresis. Plasma from normal dogs and those with hemophilia A had vWF activity, vWF:Ag, and a full range of vWF:Ag multimers on gel electrophoresis equivalent to normal canine plasma pool. Platelet cytosol contents were isolated by freezing and thawing, triton X-100 solubilization, or sonication of washed platelets with and without protease inhibitors and inhibitors of platelet activation. Washed platelets were also stimulated with calcium ionophore and MgCl2. There was no measurable vWF activity or vWF:Ag in platelet lysates or releasates in any dog regardless of phenotype. All dogs were studied using a standard arterial stenosis and injury procedure to induce arterial thrombosis. Thromboses were detected by cyclic reductions in Doppler blood flow velocity. Vessels were examined by light and scanning electron microscopy. Thrombosis developed in the arteries of normal (9 of 10) and hemophilia A dogs (16 of 16) but in none of the vWD dogs (0 of 10). Infusion of canine vWF cryoprecipitate into vWD dogs markedly shortened bleeding time but did not support thrombosis as seen in dogs with vWF in the plasma and subendothelium. Thrombosis, then, fails to occur when vWF is absent from the plasma and subendothelial compartments or present only in the plasma compartment. These data are consistent with the hypothesis that vWF in the plasma and subendothelium supports thrombosis. Neither plasma FVIII nor platelet vWF is essential for thrombosis in this model. 相似文献
108.
Liu Y; Egyhazi S; Hansson J; Bhide SV; Kulkarni PS; Grafstrom RC 《Carcinogenesis》1997,18(10):1889-1895
Extracts prepared from tissue specimens of normal, non-tumourous human
buccal mucosa, and cultured buccal epithelial cells and fibroblasts,
exhibited O6-methylguanine-DNA methyltransferase (MGMT) activity by
catalysing the repair of the premutagenic O6-methylguanine lesion in
isolated DNA with rates of 0.2 to 0.3 pmol/mg protein. An SV40 T
antigen-immortalized buccal epithelial cell line termed SVpgC2a and a
buccal squamous carcinoma line termed SqCC/Y1, both of which lack normal
tumour suppressor gene p53 function, exhibited about 50 and 10% of the MGMT
activity of normal cells, respectively. The normal, experimentally
transformed and tumourous buccal cell types showed MGMT mRNA levels which
correlated with their respective levels of MGMT activity. Exposure of
buccal cell cultures to various organic or water- based extracts of
products related to the use of tobacco and betel quid, decreased both cell
survival (measured by reduction of tetrazolium dye) and MGMT activity
(measured subsequently to the exposures in cellular extracts). Organic
extracts of bidi smoke condensate and betel leaf showed higher potency than
those of tobacco and snuff. An aqueous snuff extract also decreased both
parameters, whereas an aqueous areca nut extract was without effect. The
well- established sulph-hydryl-reactive agent Hg2+, a corrosion product of
dental amalgam, served as a positive control and decreased MGMT activity
following treatment of cells within a range of 1-10 microM. Taken together,
significant MGMT activities were demonstrated in buccal tissue specimens
and in the major buccal mucosal cell types in vitro. Lower than normal MGMT
activity in two transformed buccal epithelial cell lines correlated with
decreased MGMT mRNA and lack of functional p53. Finally, in vitro
experiments suggested the potential inhibition of buccal mucosal MGMT
activity by complex mixtures present in the saliva of tobacco and betel nut
chewers.
相似文献
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