低频弱脉冲电磁场作用下成骨细胞的实时表面增强拉曼光谱研究

目的:弱强度的低频电磁场可促进成骨细胞的增殖、分化和钙盐分泌,进而加速伤骨愈合、加快骨组织代谢,为揭示低频弱脉冲电磁场影响成骨细胞的初始效应,应用表面增强拉曼光谱技术探索电磁场对成骨细胞的作用机制。方法:用出生5d的Wistar品系小鼠的颅骨接种成骨细胞并传代。应用表面增强拉曼光谱技术实时的方法采集不同磁场作用时间内低频率(50Hz)、低强度(0.30mT)脉冲电磁场作用下的成骨细胞的拉曼光谱。每隔1min采集一次,至作用8min止;并与电磁场作用前采集的拉曼光谱做比对。结果:比较电磁场作用前后和作用8min内不同时刻的拉曼光谱,可知其对成骨细胞膜蛋白和磷脂分子的构象有一定影响:表征膜蛋白中氨基酸侧链和磷脂C-C键骨架的特征峰的位移只发生了小幅变化,说明实验电磁场对细胞膜无损伤作用;在其作用之初的4.0～5.0min内,表征蛋白质构象信息的6个拉曼特征峰的相对强度随时间逐渐增加,揭示了构象发生改变的膜蛋白数量随电磁场作用时间的延长而增多。结论:实验探测到成骨细胞对低频脉冲电磁场的最初响应机制,表现为膜蛋白构象的有序变化。这为进一步探索由电磁刺激而激活的成骨细胞一系列信号转导通路的上游机制提供了有力的依据。     

电流作用后成骨细胞表面增强的拉曼光谱

目的:多种实验已获知弱强度的电流可促进成骨细胞的增殖、分化和钙盐分泌,进而加速骨伤愈合,加快骨组织代谢。利用表面增强拉曼光谱,可在单细胞水平观察电流刺激下成骨细胞质膜蛋白结构的变化。方法:实验于2006-06/08在南开大学泰达应用物理学院完成。出生4d的昆明品系乳鼠6只,体质量约50g,将1×109L-1密度的成骨细胞用2.5g/L胰酶消化下来,置入到5mmol/L葡萄糖溶液(D-Hanks配置)中。做拉曼光谱的时候取出100μL含有成骨细胞的D-Hanks溶液,使一些成骨细胞固定到CaF2载玻片上。然后应用表面增强拉曼光谱技术,采集0.4mA和1.3mA不同电流强度作用下成骨细胞的拉曼光谱,并与电磁场作用前采集的拉曼光谱做对比。结果:①成骨细胞加电流前后,分别得到30个细胞的拉曼光谱,并利用软件origin7.0算出拉曼光谱的平均相对强度。②根据电流作用前10个细胞的拉曼光谱的相对峰值的平均值和电流作用后10个细胞的拉曼光谱的相对峰值的平均值,得出成骨细胞在受电流作用后,拉曼光谱中11个强峰的相对强度有明显的下降,表明在电流的作用后,成骨细胞膜蛋白构象有很大的变化。③表征蛋白质C-N伸缩振动的谱线1132cm-1的相对强度,在受两种电流作用后,分别增加了128.57%、57.14%,表明细胞质膜蛋白从有序状态转变为无序状态。结论:成骨细胞质膜蛋白在经过电流作用后,细胞膜结构发生较大变化,电流对稳定蛋白质二级结构的氢键和盐键有明显的影响。并且预示拉曼光谱有做为一种潜在的研究骨组织疾病诊断工具的可能性。     

中药独活活性成分香豆素及其甙的化学研究

独活为伞形科重齿毛当归(Angelica pubescens Maxim.F.biserrataShan et Yuan)的根及根茎,为常用中药,有祛风通络,活血舒筋,宜通百脉的作用。治腰膝酸痛,手脚挛痛。本研究证明独活有抑制血小板聚集抗血栓形成的作用,可能为独活善行血分,活血舒筋,宜通百脉的作用机制之一。我们将湖北资丘产独活以CH_2Cl_2,C_2H_5OH及H_2O提取,各提取部位分别按Born     

Structural and compositional modifications of diabetic low-density lipoproteins influence their receptor-mediated uptake by hepatocytes

Dyslipoproteinaemia is an important risk factor for the development of atherosclerosis in non-insulin-dependent diabetes mellitus (NIDDM). This study shows that the uptake of low-density lipoproteins (LDLs) prepared from the plasma of patients with NIDDM in cultured human hepatoma cells is largely reduced. In addition, diabetic LDL was less effective in suppressing intracellular cholesterol synthesis. This is because of physicochemical and biochemical differences between lipoproteins from diabetic and from normal individuals. LDL from patients with NIDDM was abnormal with regard to charge, the degree of glycation, the lipid composition and the conformation of the apolipoprotein B receptor-binding domain. The diminished receptor-mediated uptake of apolipoprotein B-containing lipoproteins in diabetic individuals most probably leads to the accumulation of these lipoproteins in vivo and may be of great importance to the pathogenesis of atheroclerosis in these patients.     

毛茛甙的抗诱变作用及代谢转化

研究表明,毛茛甙可显著抑制MMC和MMS等诱变剂对沙门氏菌TA₁₀₀和TA102回复突变作用;对MMC诱发的小鼠PEC徽核抑制率达56.5%。代谢研究表明:毛茛甙抑制DNA生物合成的作用可被肝微粒体酶和胞浆液分别降低46和12%;RP-HPLC测定显示,体外毛茛甙可被肝微粒代谢转化。     

Perinatal distress in 1p36 deletion syndrome can mimic hypoxic ischemic encephalopathy

1p36 deletion syndrome is a well‐described condition with a recognizable phenotype, including cognitive impairment, seizures, and structural brain anomalies such as periventricular leukomalacia (PVL). In a large series of these individuals by Battaglia et al., “birth history was notable in 50% of the cases for varying degrees of perinatal distress.” Given the potential for perinatal distress, seizures and PVL, we questioned if this disorder has clinical overlap with hypoxic ischemic encephalopathy (HIE). We reviewed the medical records of 69 individuals with 1p36 deletion to clarify the perinatal phenotype of this disorder and determine if there is evidence of perinatal distress and/or hypoxic injury. Our data provides evidence that these babies have signs of perinatal distress. The majority (59% term; 75% preterm) needed resuscitation and approximately 18% had cardiac arrest. Most had abnormal brain imaging (84% term; 73% preterm) with abnormal white matter findings in over half of patients. PVL or suggestion of “hypoxic insult” was present in 18% of term and 45% of preterm patients. In conclusion, individuals with 1p36 deletion have evidence of perinatal distress, white matter changes, and seizures, which can mimic HIE but are likely related to their underlying chromosome disorder.     

Barratt冲动量表中文版在209名大三学生中的试用

目的：探索Barratt冲动量表（BIS-11）的信度和效度。方法：对209名在校大学生进行Barratt冲动量表测查，并间隔一个月进行重测。结果：条目分析结果表明，除条目9、12、22、25外，其他条目-总分相关r为0．16-0．58（P〈0．05，P〈0．01）；重测信度为0．83（P〈0．001）；探索性因素分析的结果得到注意、运动和缺少计划三个因子（特征根大于1，解释方差比率总计52．64％），除条目18、22和27外，其他条目的负荷范围在0．30～0．62之间；验证性因素分析GFI、AGFI、NNFI和CFI分别为0．91、0．85、0．75、0．80，RMSEA为0．09；量表及因子α系数为：注意0．73，运动0．60，缺少计划0．65，总分0．80。结论：BIS-11是一个比较好的评估冲动行为的工具。     

吲哚美辛缓释片制备工艺及体外溶出特性的研究

目的：采用新型骨架材料甲壳胺制备吲哚辛缓释片。方法：单因素考察法考察工艺因素如压力及甲壳胺粒度对缓释作用的影响,利用正交设计优化处方。结果：甲壳胺粒度小于１５４μｍ 时,抗张强度在（４ ．０ ±１０ ．０）ｋｇ 范围内,对甲壳胺缓释作用无明显影响,当抗张强度大于１３ｋｇ ,时,药物释放明显减慢,优化后的处方可达到美国药典ⅩⅩⅡ版对缓释制剂体外溶出的要求。结论：该片剂处方合理,制备工艺简单易行,适合工业化大生产。     

Effects of biologically modified surfaces of synthetic lenticules on corneal epithelialization in vivo

Purpose: The present study investigated the association of extracellular matrix (ECM) glycoprotein coatings with initial epithelialization of artificial lenticules in vivo. Methods: Collagen I, collagen IV, ECM and fibronectin were individually coated onto the surface of polycarbonate membranes. The membranes were then implanted in the anterior stroma of adult cats and were clinically assessed for rapidity and extent of epithelialization and the persistence of epithelial attachment. Results/Conclusions: It was demonstrated that membrane surfaces modified by collagen I, collagen IV and ECM consistently supported initial migration and attachment of corneal epithelial cells and that the surface modified with collagen I performed best.     

毛茛甙体外细胞毒活性及其机制

研究表明,毛茛甙对KB细胞、Bel_(7402)细胞的IC_(50)分别为0.21和0.35 μmol/L;对细胞大分子物质的生物合成以抑制DNA合成最强(IC_(50)=0.35μmol/L)。作用机制研究表明,至少有两种机制参与毛茛甙的体外细胞毒作用:抑制DNA聚合酶作用下的DNA合成;促进超氧阴离子自由基的生成。