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排序方式: 共有355条查询结果,搜索用时 15 毫秒
91.
92.
Renata MB Peres Cláudia RC Costa Paula D Andrade Sandra HA Bonon Dulcinéia M Albuquerque Cristiane de Oliveira Afonso C Vigorito Francisco JP Aranha Cármino A de Souza Sandra CB Costa 《BMC infectious diseases》2010,10(1):147
Background
Human cytomegalovirus (CMV) infection still causes significant morbidity and mortality after allogeneic hematopoietic stem cell transplantation (HSCT). Therefore, it is extremely important to diagnosis and monitor active CMV infection in HSCT patients, defining the CMV DNA levels of virus replication that warrant intervention with antiviral agents in order to accurately prevent CMV disease and further related complications. 相似文献93.
S Nicholls P Domizio CB Williams A Dawnay CP Braegger TT MacDonald JA Walker-Smith 《Archives of disease in childhood》1994,71(3):243-247
Childhood Crohn's disease may cause significant morbidity. T cell activation is considered to be central to Crohn's disease pathology, and as cyclosporin is a powerful inhibitor of T cell activation, and has been used in adult Crohn's disease with encouraging results, it may offer the prospect of remission if given early in the course of disease. Children with newly diagnosed Crohn's disease or those relapsing off treatment were therefore given cyclosporin or conventional treatment (enteral nutrition or corticosteroids) by random allocation. Evaluation was performed initially and at two months. Twenty four children were studied (10 on cyclosporin and 14 on conventional treatment; one child on cyclosporin withdrew). Significant clinical improvement occurred in the group on conventional treatment, but not in the cyclosporin group. Colonoscopic improvement was noted in 5/9 on cyclosporin and 8/14 on conventional treatment, but neither group produced a significant fall in median colonoscopic index. Histological improvement was seen in 7/8 on cyclosporin and 8/13 on conventional treatment, but cyclosporin was not significantly better. Cyclosporin produced improved clinical and histological appearance without matched improvement in blood disease indices. It was not better than conventional treatment, and simple oral administration is probably not suitable for newly diagnosed patients with Crohn's disease. 相似文献
94.
Insulin-like growth factor (IGF)-I and IGF binding protein-3 concentrations in fluid from human stimulated follicles 总被引:2,自引:5,他引:2
Oosterhuis GJ; Vermes I; Lambalk CB; Michgelsen HW; Schoemaker J 《Human reproduction (Oxford, England)》1998,13(2):285-289
Insulin-like growth factor-I (IGF-I) and IGF binding protein-3 (IGFBP- 3)
play an important role in regulating follicle growth and maturation. We
have evaluated whether responsiveness to gonadotrophins during an in- vitro
fertilization (IVF) treatment is related to follicular fluid IGF- I and
IGFBP-3 concentrations. We also investigated if a difference is present in
IGF-I and IGFBP-3 concentrations between patients treated with human
menopausal gonadotrophin (HMG) and patients treated with highly purified
follicle stimulating hormone (FSH). We have measured IGF-I and IGFBP-3 in
follicular fluid from pre-ovulatory follicles in an IVF programme. All 70
patients were stimulated after being down- regulated with a
gonadotrophin-releasing hormone (GnRH) analogue. IGF-I concentrations in
follicular fluid were significantly inversely correlated with the number of
ampoules FSH administered and number of days of FSH administration, and
significantly correlated with the number of follicles aspirated. IGFBP-3
concentrations were not correlated with any other parameter measured nor
were IGF-I and IGFBP-3 concentrations correlated. IGFBP-3 concentrations
were significantly higher in patients receiving highly purified FSH
compared with patients receiving HMG (P < 0.005). These results are new
evidence that IGF-I concentration in follicular fluid is higher in women
who respond better to follicular stimulation, i.e. women who grow many
follicles, women who need a shorter duration of stimulation and women who
need fewer ampoules FSH before oocyte retrieval.
相似文献
95.
Altered metabolism of familial Alzheimer's disease-linked amyloid precursor protein variants in yeast artificial chromosome transgenic mice 总被引:4,自引:3,他引:4
Lamb BT; Call LM; Slunt HH; Bardel KA; Lawler AM; Eckman CB; Younkin SG; Holtz G; Wagner SL; Price DL; Sisodia SS; Gearhart JD 《Human molecular genetics》1997,6(9):1535-1541
Missense mutations in the beta-amyloid precursor protein gene (APP) co-
segregate with a small subset of autosomal dominant familial Alzheimer's
disease (FAD) cases wherein deposition of the 39-43 amino acid beta-amyloid
(A beta) peptide and neurodegeneration are principal neuropathological
hallmarks. To accurately examine the effect of missense mutations on APP
metabolism and A beta production in vivo, we have introduced yeast
artificial chromosomes (YACs) containing the entire approximately 400 kbp
human APP gene encoding APP harboring either the asparagine for lysine and
leucine for methionine FAD substitution at codons 670 and 671
(APP(K670N/M671L)), the isoleucine for valine FAD substitution at codon 717
(APP(V7171)) or a combination of both substitutions into transgenic mice.
We demonstrate that, relative to YAC transgenic mice expressing wild-type
APP, high levels of A beta peptides are detected in the brains of YAC
transgenic mice expressing human APP(K670N/M671L) that is associated with a
concomitant diminution in the levels of apha-secretase-generated soluble
APP derivatives. Moreover, the levels of longer A beta peptides (species
terminating at amino acids 42/43) are elevated in YAC transgenic mice
expressing human APP(V7171). These mice should prove valuable for detailed
analysis of the in vivo effects of the APP FAD mutations in a variety of
tissues and throughout aging and for testing therapeutic agents that
specifically alter APP metabolism and A beta production.
相似文献
96.
97.
Autosomal recessive retinitis pigmentosa and cone-rod dystrophy caused by splice site mutations in the Stargardt's disease gene ABCR 总被引:12,自引:2,他引:12
Cremers FP; van de Pol DJ; van Driel M; den Hollander AI; van Haren FJ; Knoers NV; Tijmes N; Bergen AA; Rohrschneider K; Blankenagel A; Pinckers AJ; Deutman AF; Hoyng CB 《Human molecular genetics》1998,7(3):355-362
Ophthalmological and molecular genetic studies were performed in a
consanguineous family with individuals showing either retinitis pigmentosa
(RP) or cone-rod dystrophy (CRD). Assuming pseudodominant (recessive)
inheritance of allelic defects, linkage analysis positioned the causal gene
at 1p21-p13 (lod score 4.22), a genomic segment known to harbor the ABCR
gene involved in Stargardt's disease (STGD) and age- related macular
degeneration (AMD). We completed the exon-intron structure of the ABCR gene
and detected a severe homozygous 5[prime] splice site mutation,
IVS30+1G->T, in the four RP patients. The five CRD patients in this
family are compound heterozygotes for the IVS30+1G- >T mutation and a
5[prime] splice site mutation in intron 40 (IVS40+5G- >A). Both splice
site mutations were found heterozygously in two unrelated STGD patients,
but not in 100 control individuals. In these patients the second mutation
was either a missense mutation or unknown. Since thus far no STGD patients
have been reported to carry two ABCR null alleles and taking into account
that the RP phenotype is more severe than the STGD phenotype, we
hypothesize that the intron 30 splice site mutation represents a true null
allele. Since the intron 30 mutation is found heterozygously in the CRD
patients, the IVS40+5G->A mutation probably renders the exon 40 5[prime]
splice site partially functional. These results show that mutations in the
ABCR gene not only result in STGD and AMD, but can also cause autosomal
recessive RP and CRD. Since the heterozygote frequency for ABCR mutations
is estimated at 0.02, mutations in ABCR might be an important cause of
autosomal recessive and sporadic forms of RP and CRD.
相似文献
98.
99.
阿霉素羧甲基葡聚糖微球犬肝动脉栓塞后阿霉素的体内过程 总被引:4,自引:0,他引:4
研究了阿霉素羧甲基葡聚糖微球经肝动脉栓塞后的体内动力学过程、靶向特征和微球在体内的肝动脉栓塞效果。对犬进行肝动脉栓塞实验,并与肝动脉阿霉素(ADM)溶液灌注组进行对照。用HPLC荧光检测外周静脉和组织中药物浓度。结果表明:微球组峰浓度为0.558μg/ml,溶液组为1.013μg/ml;微球组的T1/2(α),T1/2(β)和MRT分别为溶液组的2.82,3.19和1.28倍。栓塞不同部位组织中ADM浓度,微球组分别是溶液组的8.0和9.1倍。动态血管造影表明:肝内外未见侧枝循环形成,栓塞作用持久,16周后微球仍未见完全降解。 相似文献
100.
Coarctation of the aorta: MR imaging 总被引:1,自引:0,他引:1
von Schulthess GK; Higashino SM; Higgins SS; Didier D; Fisher MR; Higgins CB 《Radiology》1986,158(2):469-474
Thirteen patients, aged 3-31 years, with coarctation of the thoracic aorta were examined by magnetic resonance (MR) imaging (total of 14 studies). Eight studies were performed preoperatively and six postoperatively. Catheterization data were available on 12 patients for verification of MR imaging findings. Electrocardiographically gated sagittal and left anterior oblique images best depicted the coarctations; however, involvement of arch vessels was best evaluated on transaxial images. MR imaging readily identified all coarctations but one, their site and extent, and involvement of the arch vessels. In addition, MR images depicted poststenotic dilatation and dilated collateral vessels. In patients studied postoperatively, restenosis could be evaluated, and complications such as postoperative aneurysm and perianastomotic hematoma were identified. MR imaging provides excellent anatomic detail of coarctation of the aorta, potentially obviating the need for angiography. 相似文献