Identification of a new HLA-B*56 variant, B*5614

In this report, the novel allele B*5614 is presented. The allele was identified in a Chinese individual by sequence-based typing. HLA-B*5614 differs from B*5608 by a single nucleotide at position 277G-->C in exon 2. This results in an amino acid change from Gly to Arg at codon 93.     

医院实施ISO9001质量管理体系内部审核的探讨

医疗机构实施ISO9001,其目的是为了提高医疗服务质量,满足病人一切明确和隐含的需求。那么,内审是否发挥作用,直接关系管理体系的有效性。文章对此进行了初步的探讨,即:①明确内审的目的是检查体系满足标准要求的程度;②建立正规的内审机构,要选拔责任心强、综合素质高的人担任内审员;③编制合理且有特色的内审计划,是内审有效性的关键;④适当增加专题审核;⑤医院最高管理者应重视内审结果。     

IL-17 Eliminates the Therapeutic Effects of Myelin Basic Protein-Induced Nasal Tolerance in Experimental Autoimmune Encephalomyelitis by Activating IL-6

Interleukin (IL)-17 is a proinflammatory cytokine primarily secreted by Th17 cells, which are a CD4⁺ T-cell subset. Th17 cells and IL-17 are important in the pathogenesis of multiple sclerosis and in its established animal model, experimental autoimmune encephalomyelitis (EAE). However, it is unclear whether IL-17 contributes to EAE immune tolerance. We used the myelin basic protein (MBP) peptide MBP 68–86 to induce nasal tolerance to EAE, and simultaneously interfered with the tolerance by treatment with different doses of IL-17. We found that IL-17 dramatically interfered with MBP 68–86-induced immune tolerance. IL-17 administration increased IL-6 release, skewing T cell differentiation towards Th17 cells and decreasing the number of Treg cells. This led to an imbalance between Treg cells and Th17 cells and spurred the development of EAE.     

Ketamine inhibits LPS-induced calcium elevation and NF-kappa B activation in monocytes

Objective:To investigate whether ketamine could inhibit lipopolysaccharide (LPS)-induced intracellular calcium elevation and NF-kappa B activation in monocytes. Materials and methods:Isolated rat monocytes were challenged with 10 g/ml LPS with or without the presence of various concentrations of ketamine (10, 100, 1000 M). Intracellular calcium was monitored by laser confocal microscopy. NF-kappa B activity of the nuclear extracts of monocytes was analyzed by electrophoretic mobility shift assay (EMSA). Results:LPS provoked a significant calcium elevation and enhanced NF-kappa B activity in monocytes. Ketamine above concentration of 100 M inhibited endotoxin-induced intracellular calcium elevation and NF-kappa B activity. Ketamine itself had no effect on either of them. Conclusions:These findings suggest that ketamine could suppress NF-kappa B in monocytes exposed to endotoxin, and this anti-inflammatory effect might act through attenuating intracellular calcium elevation.Received 31 October 2003; returned for revision 18 December 2003; accepted by I. Ahnfelt-Rønne 26 Januaryy 2004     

Pyriform sinus fistula in children: A comparison of endoscopic-assisted surgery and endoscopic radiofrequency ablation

BackgroundPyriform sinus fistula (PSF) is a rare congenital anomaly, and the preferred definitive treatment is yet to be verified. In this study, we investigated the treatment outcomes of PSF specifically comparing endoscopic-assisted surgery and endoscopic radiofrequency ablation (RA).MethodsThe medical records of patients treated for PSF at the Shanghai Children's Hospital between October 2016 and September 2019 were retrospectively evaluated.ResultsThere were 93 girls and 98 boys. The median age at onset and operation was 3 years and 5 years, respectively. Endoscopic-assisted surgery was performed in 143 patients. During the same period, RA was performed in 48 patients, and 10 of them concurrently underwent incision and drainage of neck abscesses. Longer hospital stay was found in the endoscopic-assisted surgery group than in the RA group (10.50 ± 3.93 vs. 5.02 ± 3.30 days, P < 0.001). Postoperative complications were not significantly different between the two groups, except for neck infection (0 vs. 8.3%, P = 0.004). After a median follow-up period of 21 months, no significant difference was found between the two groups in terms of recurrence (1.4% vs. 0, P = 0.560).ConclusionPatients treated with RA had a significantly shorter hospital stay than those treated with endoscopic-assisted surgery. Outcomes of endoscopic-assisted surgery and RA were not significantly different for the management of PSF and treatment method should be tailored to the patient.Level of evidenceIV.     

不育男性精浆转铁蛋白含量与精子顶体形态的关系

目的 :探讨不育男性精浆转铁蛋白 (Tf)含量与精子顶体完整性的关系。方法 :149例不育男性患者按精子密度≥ 2 0× 10 6 / ml和 <2 0× 10 6 / ml分正常精子密度组和少精子组 ,同时进行精浆 Tf和精子顶体完整率 (PIA)分析。结果 :不育男性少精子组精浆 Tf含量显著低于正常精子密度组 (P<0 .0 1) ;正常精子密度的不育男性精子 PIA<80 %组精浆 Tf含量显著低于精子 PIA≥ 80 %组 (P<0 .0 1)。结论 :正常精子密度的不育男性精子 PIA下降与精浆 Tf水平有密切的关系     

注射用阿莫西林钠克拉维酸钾细菌内毒素检查

目的建立注射用阿莫西林钠克拉维酸钾细菌内毒素检查方法.方法按中国药典2000年版附录细菌内毒素检查法要求进行试验.结果注射用阿莫西林钠克拉维酸的浓度为2.5mg/ml时不干扰鲎试剂与细菌内毒素的凝胶反应.结论细菌内毒素检查法适用于检测注射用阿莫西林钠克拉维酸钾中的内毒素.     

Effects of histaminergic neuron on the developmental process of conditioned reward induced by morphine

To investigate the effect of histaminergic neuron on morphine - induced conditioned place preference （ CPP）, the model of CPP was used to assess the rewarding effect of morphine. Morphine （2, 5, 10 mg/kg） significantly produced a CPP in a dose-dependent manner. In addition, morphine simultaneously and markedly lessened the histaminergic neurons in the TM,     

支气管动脉灌注化疗及静脉化疗联合放疗对Ⅲ期非小细胞肺癌疗效的比较

目的 通过对支气管动脉灌注化疗及静脉化疗联合放疗对Ⅲ期非小细胞肺癌的疗效及N分期对其疗效的影响的研究,为临床选择支气管动脉灌注化疗或静脉化疗提供理论依据.方法 70例患者采用单盲随机分为支气管动脉灌注化疗联合放疗组（A组）37例及静脉化疗联合放疗组（B组）33例.结果 A、B组近期有效率分别为78.4%和57.6%,差异无显著性（P=0.06）;1、2、3年生存率分别为64.9%、24.3%、16.2%及51.5%、18.2%、12.1%,差异均无显著性（P＞0.05）.按UICC（国际抗癌联盟）TNM分期中N分期进行分层研究,两组中N0～N1者A组23例,B组17例,近期有效率分别为91.3%及64.7%,差异有显著性（P＜0.05）,1、2、3年生存率分别为78.3%、30.4%、21.7%及58.8%、23.5%、11.7%,差异均无显著性（P＞0.05）.N2～N3者A组14例,B组16例,近期有效率分别为57.1%及50.0%,差异无显著性（P＞0.05）;两组1、2、3年生存率分别为42.9%、14.3%、7.1%及43.8%、12.5%、12.5%,差异均无显著性（P＞0.05）.结论 支气管动脉灌注化疗联合放疗能提高Ⅲ期N0～N1非小细胞肺癌患者的近期疗效,但生存期两者差异无显著性,而对于N2～N3患者无论近期疗效还是生存期差异均无显著性.