全文获取类型
收费全文 | 2431篇 |
免费 | 200篇 |
国内免费 | 3篇 |
专业分类
耳鼻咽喉 | 32篇 |
儿科学 | 85篇 |
妇产科学 | 49篇 |
基础医学 | 332篇 |
口腔科学 | 29篇 |
临床医学 | 329篇 |
内科学 | 422篇 |
皮肤病学 | 31篇 |
神经病学 | 176篇 |
特种医学 | 30篇 |
外科学 | 283篇 |
综合类 | 76篇 |
预防医学 | 355篇 |
眼科学 | 16篇 |
药学 | 159篇 |
中国医学 | 4篇 |
肿瘤学 | 226篇 |
出版年
2023年 | 26篇 |
2022年 | 42篇 |
2021年 | 76篇 |
2020年 | 50篇 |
2019年 | 62篇 |
2018年 | 65篇 |
2017年 | 66篇 |
2016年 | 57篇 |
2015年 | 77篇 |
2014年 | 84篇 |
2013年 | 156篇 |
2012年 | 195篇 |
2011年 | 184篇 |
2010年 | 105篇 |
2009年 | 107篇 |
2008年 | 158篇 |
2007年 | 143篇 |
2006年 | 142篇 |
2005年 | 138篇 |
2004年 | 139篇 |
2003年 | 127篇 |
2002年 | 121篇 |
2001年 | 23篇 |
2000年 | 19篇 |
1999年 | 26篇 |
1998年 | 12篇 |
1997年 | 23篇 |
1996年 | 23篇 |
1995年 | 16篇 |
1994年 | 15篇 |
1993年 | 9篇 |
1992年 | 13篇 |
1991年 | 11篇 |
1990年 | 7篇 |
1989年 | 7篇 |
1988年 | 11篇 |
1987年 | 5篇 |
1986年 | 7篇 |
1985年 | 10篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 5篇 |
1979年 | 5篇 |
1976年 | 4篇 |
1972年 | 4篇 |
1971年 | 3篇 |
1970年 | 3篇 |
1969年 | 5篇 |
排序方式: 共有2634条查询结果,搜索用时 38 毫秒
101.
Maria Negahdar Ingvild Aukrust Janne Molnes Marie H. Solheim Bente B. Johansson Jørn V. Sagen Knut Dahl-Jørgensen Rohit N. Kulkarni Oddmund Søvik Torgeir Flatmark Pål R. Njølstad Lise Bjørkhaug 《Molecular and cellular endocrinology》2014
GCK-MODY, dominantly inherited mild hyperglycemia, is associated with more than 600 mutations in the glucokinase gene. Different molecular mechanisms have been shown to explain GCK-MODY. Here, we report a Pakistani family harboring the glucokinase mutation c.823C > T (p.R275C). The recombinant and in cellulo expressed mutant pancreatic enzyme revealed slightly increased enzyme activity (kcat) and normal affinity for α-D-glucose, and resistance to limited proteolysis by trypsin comparable with wild-type. When stably expressed in HEK293 cells and MIN6 β-cells (at different levels), the mutant protein appeared misfolded and unstable with a propensity to form dimers and aggregates. Its degradation rate was increased, involving the lysosomal and proteasomal quality control systems. On mutation, a hydrogen bond between the R275 side-chain and the carbonyl oxygen of D267 is broken, destabilizing the F260-L271 loop structure and the protein. This promotes the formation of dimers/aggregates and suggests that an increased cellular degradation is the molecular mechanism by which R275C causes GCK-MODY. 相似文献
102.
103.
Lise M Verhoef Tom H Van de Belt Lucien JLPG Engelen Lisette Schoonhoven Rudolf B Kool 《Journal of medical Internet research》2014,16(2)
Background
Insight into the quality of health care is important for any stakeholder including patients, professionals, and governments. In light of a patient-centered approach, it is essential to assess the quality of health care from a patient’s perspective, which is commonly done with surveys or focus groups. Unfortunately, these “traditional” methods have significant limitations that include social desirability bias, a time lag between experience and measurement, and difficulty reaching large groups of people. Information on social media could be of value to overcoming these limitations, since these new media are easy to use and are used by the majority of the population. Furthermore, an increasing number of people share health care experiences online or rate the quality of their health care provider on physician rating sites. The question is whether this information is relevant to determining or predicting the quality of health care.Objective
The goal of our research was to systematically analyze the relation between information shared on social media and quality of care.Methods
We performed a scoping review with the following goals: (1) to map the literature on the association between social media and quality of care, (2) to identify different mechanisms of this relationship, and (3) to determine a more detailed agenda for this relatively new research area. A recognized scoping review methodology was used. We developed a search strategy based on four themes: social media, patient experience, quality, and health care. Four online scientific databases were searched, articles were screened, and data extracted. Results related to the research question were described and categorized according to type of social media. Furthermore, national and international stakeholders were consulted throughout the study, to discuss and interpret results.Results
Twenty-nine articles were included, of which 21 were concerned with health care rating sites. Several studies indicate a relationship between information on social media and quality of health care. However, some drawbacks exist, especially regarding the use of rating sites. For example, since rating is anonymous, rating values are not risk adjusted and therefore vulnerable to fraud. Also, ratings are often based on only a few reviews and are predominantly positive. Furthermore, people providing feedback on health care via social media are presumably not always representative for the patient population.Conclusions
Social media and particularly rating sites are an interesting new source of information about quality of care from the patient’s perspective. This new source should be used to complement traditional methods, since measuring quality of care via social media has other, but not less serious, limitations. Future research should explore whether social media are suitable in practice for patients, health insurers, and governments to help them judge the quality performance of professionals and organizations. 相似文献104.
105.
Lara Benmeridja Lise De Moor Elisabeth De Maere Florian Vanlauwe Michelle Ryx Liesbeth Tytgat Chris Vercruysse Peter Dubruel Sandra Van Vlierberghe Phillip Blondeel Heidi Declercq 《Journal of tissue engineering and regenerative medicine》2020,14(6):840-854
For patients with soft tissue defects, repair with autologous in vitro engineered adipose tissue could be a promising alternative to current surgical therapies. A volume‐persistent engineered adipose tissue construct under in vivo conditions can only be achieved by early vascularization after transplantation. The combination of 3D bioprinting technology with self‐assembling microvascularized units as building blocks can potentially answer the need for a microvascular network. In the present study, co‐culture spheroids combining adipose‐derived stem cells (ASC) and human umbilical vein endothelial cells (HUVEC) were created with an ideal geometry for bioprinting. When applying the favourable seeding technique and condition, compact viable spheroids were obtained, demonstrating high adipogenic differentiation and capillary‐like network formation after 7 and 14 days of culture, as shown by live/dead analysis, immunohistochemistry and RT‐qPCR. Moreover, we were able to successfully 3D bioprint the encapsulated spheroids, resulting in compact viable spheroids presenting capillary‐like structures, lipid droplets and spheroid outgrowth after 14 days of culture. This is the first study that generates viable high‐throughput (pre‐)vascularized adipose microtissues as building blocks for bioprinting applications using a novel ASC/HUVEC co‐culture spheroid model, which enables both adipogenic differentiation while simultaneously supporting the formation of prevascular‐like structures within engineered tissues in vitro. 相似文献
106.
Elias Badal Rashu Anders Ellekær Junker Karen Vagner Danielsen Emilie Dahl Ole Hamberg Line Borgwardt Vibeke Brix Christensen Nicolai J Wewer Albrechtsen Lise L Gluud 《World Journal of Clinical Cases》2020,8(9):1642-1650
BACKGROUND Cholesteryl ester storage disease(CESD)is a rare genetic disease.Its symptoms and severity are highly variable.CESD is a systemic disease that can lead to the accumulation of fat and inflammation in the liver,as well as gastrointestinal and cardiovascular disease.The majority of patients require liver transplantation due to decompensated cirrhosis.Enzyme replacement therapy has been approved based on a randomized trial.Our study aims to clinically and genetically evaluate two siblings with CESD who underwent liver transplantation,as well as their first-degree family members.CASE SUMMARY The siblings were compound heterozygous for the missense variant in LIPA exon 8,c.894G>A,(p.Gln298Gln)and a single base pair deletion,c.482del(p.Asn161Ilefs*19).Analyses of single nucleotide polymorphisms showed variants with an increased risk of fatty liver disease and fibrosis for both patients.Clinically,both patients show signs of recurrence of CESD in the liver after transplantation and additional gastrointestinal and cardiovascular signs of CESD.Three family members who were LIPA heterozygous had a lysosomal acid lipase activity below the reference value.One of these carriers,a seven-year-old boy,was found to have severe dyslipidemia and was subsequently treated with statins.CONCLUSION Our study underlines that CESD is a multi-organ disease,the progression of which may occur post-liver transplantation.Our findings underline the need for monitoring of complications and assessment of possible further treatment. 相似文献
107.
108.
109.
Åke Borg Robert W. Haile Kathleen E. Malone Marinela Capanu Ahn Diep Therese Törngren Sharon Teraoka Colin B. Begg Duncan C. Thomas Patrick Concannon Lene Mellemkjaer Leslie Bernstein Lina Tellhed Shanyan Xue Eric R. Olson Xiaolin Liang Jessica Dolle Anne‐Lise Børresen‐Dale Jonine L. Bernstein 《Human mutation》2010,31(3):E1200-E1240
BRCA1 and BRCA2 screening in women at high‐risk of breast cancer results in the identification of both unambiguously defined deleterious mutations and sequence variants of unknown clinical significance (VUS). We examined a population‐based sample of young women with contralateral breast cancer (CBC, n=705) or unilateral breast cancer (UBC, n=1398). We identified 470 unique sequence variants, of which 113 were deleterious mutations. The remaining 357 VUS comprised 185 unique missense changes, 60% were observed only once, while 3% occurred with a frequency of >10%. Deleterious mutations occurred three times more often in women with CBC (15.3%) than in women with UBC (5.2%), whereas combined, VUS were observed in similar frequencies in women with CBC and UBC. A protein alignment algorithm defined 16 rare VUS, occurring at highly conserved residues and/or conferring a considerable biochemical difference, the majority located in the BRCA2 DNA‐binding domain. We confirm a multiplicity of BRCA1 and BRCA2 VUS that occur at a wide range of allele frequencies. Although some VUS inflict chemical differences at conserved residues, suggesting a deleterious effect, the majority are not associated with an increased risk of CBC. © 2010 Wiley‐Liss, Inc. 相似文献