宣肺定喘汤治疗咳嗽变异性哮喘30例观察

咳嗽变异性哮喘又名咳嗽型哮喘、隐匿性哮喘，是哮喘的一种特殊类型，其特殊性在于几乎没有喘息或呼吸困难，而以长期反复发作性干咳为主要临床表现。2003年10月-2005年12月，笔者自拟宣肺定喘汤治疗咳嗽变异性哮喘30例取得较满意疗效，现报告如下。     

激光散射浊度法测定人血清中α_1AGP在癌症诊断中的应用

本文通过激光散射浊度法及火箭电泳法对健康人及癌症患者血清中α_1—酸性糖蛋白(简称α_1AGP)的分析表明,这两种方法均能准确地定量分析.α_1AGP的含量,而激光散射浊度法又有其独特的优点,其分析结果显示:未经治疗的胃、贲门、食管及肺癌患者血清中α_1AGP含量明显高于正常人,其阳性率分别为100％(15/15),90％(19/21),74％(20/27)和84％(27/32),当各种癌症病人经放化疗后,其α_1AGP含量下降,乃至正常.测定单位使用每克血清蛋白中α_1AGP的毫克数,最小检出量为1.0μg/ml,批内变异2.47％,批间变异2.77％.     

中药白屈菜生物碱含量变化规律的研究

采用高效液相色谱法对中药白屈菜的不同采收季节、不同产地、不同植物部位、不同贮存时间的样品进行了分析，测定了其中８个主要有效成分的含量，通过比较测定结果得出了结论。     

肥胖人痰湿型体质的血液流变学及甲皱微循环研究

检测肥胖人痰湿型体质、非痰湿型体质和正常人三组的血液流变学及甲皱微循环指标，结果表明痰湿体质组高于正常组（Ｐ＜０．０５），痰湿体质组与非痰湿体质组比较，全血粘度的低切率值和红细胞电泳时间高于非痰湿体质组（Ｐ＜０．０５）及流态异常增多（Ｐ＜０；０１）；管拌周围渗出增多（Ｐ＜０。０５），甲皱微循环研究提示痰湿体质者存在微循环障碍，与非痰湿体质比较存在一定差异，本研究佐证了“痰可挟瘀”、“痰可致瘀”说。     

IL-2的体内分布及其与疗效的关系

本文利用放射性核素Tc-99M标记的IL-2进行IL-2血浆半寿期及其体内分布的研究。结果显示IL-2血浆半寿期仅为10分钟,主要是由于IL-2选择性分布于肝,肾等靶器官,而不是被消除。本文结果显示,IL-2是一高选择性药物,其血浆半寿期很短,而肝,肾等靶器官浓度很高,用于治疗肝肾肿瘤时,具有疗效高而全身毒副作用低的优点。     

WHOLE ORGAN SUBSERIAL SECTION EXAMINATIONOF OCCULT BREAST CARCINOMA

ＷＨＯＬＥＯＲＧＡＮＳＵＢＳＥＲＩＡＬＳＥＣＴＩＯＮＥＸＡＭＩＮＡＴＩＯＮＯＦＯＣＣＵＬＴＢＲＥＡＳＴＣＡＲＣＩＮＯＭＡＮｉｕＹｕｎ牛昀；ＦｕＸｉｌｉｎ傅西林；ＦａｎＹｕ范宇；ＦａｎｇＺｈｉｙｉ方志沂（ＴｉａｎｊｉｎＣａｎｃｅｒＨｏｓｐｉｔａｌａｎｄ...     

Oral mucosal absorption of midazolam in dogs is strongly pH dependent

A significant amount of orally administered midazolam, an anxiolytic and sedative, may be absorbed by the mucosal membranes in the oral cavity, esophagus, and the stomach. The pH dependence of the opening or closure of a ring in midazolam's molecular structure suggests a pH dependence of the mucosal absorption, which was evaluated using a dog model. Five milliliters of 5 mg/mL midazolam solution, with a pH of 2.8, 3.2, or 3.9, was placed on the buccal mucosa of seven anesthetized dogs for 15 min. The same dogs were also infused intravenously (iv) with midazolam on separate days. Mean serum Cmax were 92.3 +/- 42.5 (mean +/- SD), 274.3 +/- 150.8, 377.1 +/- 211.3, and 2552.4 +/- 1305.3 ng/mL for pH 2.8, 3.2, 3.9 solutions and iv infusion, respectively. Mean t(max) for all buccal solutions and iv infusion was 15 min. Bioavailability for the pH 2.8, 3.2, and 3.9 solutions were 6.2, 18.7, and 22.6%, respectively. Because the commercially available midazolam syrup and the midazolam-fruit juice blends for oral administration have a pH of 2.8 to 3, these results suggest that the absorption from the blends or syrup may be significantly improved by slightly increasing the pH.     

Antitumor-promoting constituents from Dioscorea bulbifera L. in JB6 mouse epidermal cells

An antitumor-promoting effect was found in the extracts/ingredients of a plant used as a traditional medicine in mainland China, using the neoplastic transformation assay of mouse epidermal JB6 cell lines. The ethyl acetate soluble fraction of 75% ethanol extract of the rhizomes of Dioscorea bulbifera L. showed an inhibitory effect against the tumor promotion of JB6 (Cl 22 and Cl 41) cells induced by a promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA). Further investigation on the constituents of the EtOAc fraction from the rhizomes revealed the chemical structure to be kaempferol-3,5-dimethyl ether (1), caryatin (2), (+)-catechin (3), myricetin (4), quercetin-3-O-galactopyranoside (5), myricetin-3-O-galactopyranoside (6), myricetin-3-O-glucopyranoside (7) and diosbulbin B (8). Constituent antitumor-promoting activities were also examined in the same way. Compounds 1-7, characterized as flavonoids with the two hydroxyl groups at C-7 and C-4', showed the most potent inhibitory effect, but there seemed to be differences in the inhibitory effect between flavonol aglycones and flavonol glycosides. Compared with (-)-epicatechin, (+)-catechin exhibited much stronger inhibitory activity which suggested that chemical stereo structures of compounds affect the efficiency of inhibition. Compound 8 showed moderate activity. The constituents with antitumor-promoting activity from this plant are reported for the first time.     

Active decay of composite excitatory postsynaptic potentials in hippocampal slices from young rats

NMDA receptor dependent synaptic plasticity was examined in hippocampal slices using a novel pharmacological pairing procedure. Field excitatory postsynaptic potentials (EPSPs) were recorded from the CA1 area of slices maintained in a low Mg(2+) solution using a stimulus rate of 0.1-0.2 Hz. The NMDA receptor antagonist 2-amino-5-phosphonovalerate (AP5) was initially included in the perfusion solution to establish baseline recording of isolated AMPA EPSPs. Washing out AP5 led to the expression of composite EPSPs, containing both AMPA and NMDA receptor mediated components. Following an initial, transient potentiation of the AMPA component, the composite responses gradually decayed for several hours, involving AMPA and NMDA components to a similar extent. This decay was input specific and could be terminated at any stage by reapplication of AP5. Subsequent long-term potentiation (LTP) reversed the effect to an extent inversely related to the degree of depression. Experiments to test the interaction with long-term depression (LTD) revealed a significant but incomplete overlap between the two depression processes. In conclusion, pairing synaptic activation at test stimulus frequency with pharmacological unblocking of NMDA receptors allows for expression of composite EPSPs that decay substantially, due to an active mechanism. The underlying process appears to be at least partly distinct from those involved in homosynaptic LTP and LTD.     

Insertion/deletion polymorphism of the ACE gene is associated with type 2 diabetes

In an attempt to examine the role of an ACE gene insertion/deletion (I/D) polymorphism in type 2 diabetes, we conducted a case-control association study among 132 couple-pairs from northern China. The genotype frequencies for II, ID, and DD were 39.8, 39.8, and 20.3%, respectively, in the case group and 44.8, 44.8, and 10.4% in the control group. The DD frequency was significantly higher in the case group than in the control group (chi(2)(1) = 4.77, P = 0.03), suggesting that the DD genotype is associated with an increased susceptibility to type 2 diabetes in our study population.