Hyperbaric oxygenation induced tolerance against focal cerebral ischemia in mice is strain dependent

SV129 or C57BL/6 mice were exposed to hyperbaric oxygenation (HBO, 5 days, 1 h every day, 100% O(2) at 3 atm absolute). One day after the 5th HBO session focal cerebral ischemia was induced. In SV129 mice, HBO induced tolerance against permanent focal cerebral ischemia (n=42, mean infarct volume reduction 27%, P=0.001), but not against transient (30 or 60 min) focal cerebral ischemia. In the C57BL/6 strain of mice, HBO did not induce tolerance against focal cerebral ischemia, even when the duration of ischemia or the HBO protocol were modified. For the first time we demonstrate that HBO can induce tolerance to focal cerebral ischemia, but this effect is strain dependent.     

超声乳化吸除及人工晶状体植入术治疗小瞳孔白内障的临床探讨

目的:探讨小瞳孔时的白内障超声乳化术的手术技巧和临床效果。方法:对23例(27眼)散瞳后小瞳孔的白内障患者采用钝性分离及非切开的牵拉法扩大瞳孔,进行原位超声乳化并人工晶状体囊袋内植入术。结果:术后随访6～12mo,所有病例术后视力较术前有不同程度的提高,术后视力0.1～0.2者5眼,0.3～0.5者14眼;0.8以上者8眼。结论:采用钝性分离及非切开的牵拉扩大瞳孔法,原位超声乳化可使小瞳孔的白内障恢复良好的视力和生理性圆瞳孔或近圆瞳孔,手术并发症少。     

羊膜加巩膜前部覆盖防治羟基磷灰石义眼座眶内植入暴露的临床分析

目的:探讨羊膜加前部巩膜覆盖对防治羟基磷灰石(HA)义眼座植入暴露的临床效果。方法:采用羊膜联合自体或异体巩膜为加固物对13例(13眼)行Ⅰ、Ⅱ期HA义眼座植入,随访12～20mo。结果:13例均获得了较好的疗效,未见其他并发症。结论:在行HA义眼座植入时采用羊膜联合前部自体或异体巩膜覆盖加固有利于结膜创口的愈合,预防创口裂开,减少义眼座暴露。     

角膜新生血管形成机制研究进展

Campbell 1949年首先提出角膜新生血管(cornealne ovascularization，CNV)是由于角膜中存在一种能扩散的可溶性因子。现在已纯化、弄清序列并克隆成功了许多多肽类因子，不少因子具有复杂的生物学作用，这里统称为血管形成调节因子，一些小分子非肽类因子的性状尚在研究中。     

Immunobiologic, cytogenetic and drug response features of a newly established cell line (SCRC-1) from renal small cell carcinoma

PURPOSE: We describe the establishment and preliminary characterization of a cell line designated SCRC-1, which was derived from a primary renal small cell carcinoma. MATERIALS AND METHODS: Continuous cultures of a primary stage IVa renal small cell carcinoma and a xenograft in nude mice derived therefrom were characterized by immunohistology, electron microscopy, immunofluorescence/flow cytometry, cytogenetic analysis, and an in vitro drug resistance assay. RESULTS: SCRC-1 cells were reactive with antibodies to NSE, chromogranin-A, bombesin, Bcl-2, CD44s, CD44v6, CD44v7 to 8, vimentin and S100 protein (predominantly beta-subunit), and were unreactive with antibodies to EMA, CD54, EGFR(R1), URO-5, URO-7, URO-8 and URO-10. A similar immunoprofile was also found in both the primary tumor and the xenograft. Cytogenetic analysis revealed the following common clonal aberrations in all 50 metaphases analyzed: 45, XX, t (X;10;18) (p11;p11;q11), -der(18)t(X;10;18), indicating the clonal nature of this neoplasm. SCRC-1 cells showed low drug resistance to cyclophosphamide, doxorubicin, gemcitabine and fluorouracil, intermediate resistance to carmustine and mitomycin-C, and extreme resistance to cisplatin. CONCLUSION: We have documented the initial characterization of SCRC-1, which may be the first cell line reported to be derived from a primary small cell carcinoma of the kidney. This cell line can be used for further studies uncovering the biology and histogenesis of this rare cancer and delineating differences among small cell carcinomas of the kidney and other histological types.     

高效液相同时测定保健食品中两种结构形式洛伐他汀的含量

目的：建立保健食品中同时测定两种结构形式洛伐他汀含量的方法。方法：用高效液相色谱法同时测定含保健食品中两种结构形式洛伐他汀的含量。结果：方法线性范围为：0．500～0．025μg，最低检出限为0．001μg，总分析时间11min。洛伐他汀平均保留时间：闭环型9．632min，开环型8．682min。4种浓度的平均回收率为：97．77％。结论：高效液相色谱法同时测定洛伐他汀浓度稳定、可靠、简便，适用于保健食品的分析。     

抗单核细胞抗体与冠心病间关系的探讨

目的研究抗单核细胞抗体与冠心病的关系。方法以健康成人外周血单核细胞(MOC)为靶抗原,用微量间接酶免疫组织化学技术检测了35例冠心病(CHD)患者,27例献血员血清中抗单核细胞抗体(AMA)。结果冠心病组和献血员组血清中抗单核细胞抗体阳性率分别为54.3%、14.8%,平均滴度分别为1∶6.05、1∶2.63。病例组与对照组比较阳性率和平均滴度都明显增加(P<0.01),CHD患者血清稀释4倍AMA阳性率为48.6%,其四种临床类型AMA阳性率有差异,但不具显著性(P>0.05)。结论抗单核细胞抗体与冠心病有一定的关系。     

34例高血压脑出血术后再出血的临床分析

目的总结高血压脑出血术后再出血的原因、诊断及防治经验,提高对本病的认识,以便及时发现处理。方法回顾我院1999年1月～2004年12月高血压脑出血术后再出血34例的临床资料。结果高血压脑出血术后再出血发生率11.9%(34/286),手术二次手术清除血肿者24例,保守治疗7例,预后良好率61.8%(21/34),预后不良率38.2%(13/34)。结论高血压脑出血术后再出血预后不良率很高,预防高血压脑出血术后再出血是治疗的关键。     

生殖道感染干预工程对农村生殖道感染妇女就医行为影响的研究

目的:评价生殖道感染干预工程对农村生殖道感染妇女就医行为的干预效果。方法:对江西省4个县随机抽取的12个乡的12个行政村进行基线调查后实施干预,干预过程中重复进行4次问卷调查、妇科检查和实验室检测,另选取这4个县的一个行政村作为对照,观察方法同干预组。全部数据应用Epidata2.0建库管理,用SPSS10.0及SAS6.12软件进行统计分析。结果:已婚育龄妇女生殖道感染症状现患率及患者无症状比例下降,就医率由干预前31·5%上升为69·0%,较对照组39·6%有明显提高,且立即就诊比例由第一次干预时的46·7%升高到第4次干预时的75·6%,患者的治愈率、好转率大幅度提高。结论:生殖道感染的干预改善了农村已婚育龄妇女的就医行为,提高了就诊率和治愈率。     

终末病案质量常见缺陷及原因分析

目的分析我院2004年与2005年病案书写质量的变化情况。方法对比分析我院2004年和2005年37432份终末病案。结果自成立医疗质量管理委员会和病案质量管理委员会以来,通过三级质控网络的监控,我院病案质量有了进一步的提高。