小鼠睾丸引带细胞的培养及己烯雌酚对培养睾丸引带细胞的影响

目的:探讨小鼠睾丸引带细胞体外培养的有效方法,并进行形态学观察。研究经典的外源性雌激素己烯雌酚(DES)体外对小鼠睾丸引带发育的影响。方法:手术放大镜解剖出3日龄雄性昆明小鼠的睾丸引带组织并进行细胞培养。台盼蓝染色检测细胞存活率,HE染色观察细胞形态。传代培养并随机分为空白对照组、溶剂对照组(DMSO组)和实验组(DES 1~4组)共6组。溶剂对照组加入DMSO,实验组分别加入0.01、0.10、1.00以及10.00μg/ml DES。分别于培养12、24、48 h后进行细胞形态学观察,CCK-8法检测睾丸引带细胞的生长情况。结果:培养睾丸引带细胞大部分为成纤维细胞型,有少数的上皮样细胞。原代细胞的存活率为85%~90%。在不同剂量DES作用后的3个不同时间段里,细胞增殖性的检测结果存在时间-剂量效应的显著差异(P<0.01)。结论:睾丸引带细胞可经一定的方法进行体外培养,外源性雌激素对睾丸引带细胞的生长有抑制作用,且呈现一定的时间-剂量效应。对培养睾丸引带细胞影响的研究是外源性雌激素影响生殖系统发育研究的一条有效的睾丸外途径。     

"难管教儿童"的心理健康与父母教养方式

目的:探讨难管教儿童的心理健康及其父母教养方式.方法:随机抽取长沙某特殊训练营学校的难管教儿童120名和普通中学生105名,用EMBU来评估父母教养方式,用SCL-90来评估难管教儿童的心理健康水平.结果:难管教儿童的心理健康水平显著低于常模.普通中学生父母教养方式的情感温暖的得分显著高于难管教儿童:而普通中学生的父亲惩罚严厉、父亲过分干涉、父亲拒绝否认、父亲过度保护、母亲干涉保护、母亲拒绝否认、母亲惩罚严厉的得分显著低于难管教儿童.结论:难管教儿童心理健康水平较低,其父母较多使用消极的教养方式,较少的使用积极教养方式.     

液基细胞学检测蒙汉族妇女宫颈病变的临床分析

目的：分析内蒙古通辽地区蒙汉族女性宫颈病变发生率和临床特征,为蒙古族妇女子宫颈癌的预防提供相关信息。方法：应用液基细胞学检查方法,分别选择2008年1月-2009年6月在我院就诊的3 085例蒙古族妇女（A组）和5 811例汉族妇女（B组）的宫颈脱落细胞进行宫颈病变筛查,依据目前国际通用的Bethesda系统（TBS）分类标准进行诊断,对筛查结果为宫颈上皮细胞异常病例进行统计学分析比较。结果：①宫颈上皮细胞异常的总发生率A组为14.17%（437/3 085）、B组为13.03%（757/5 811）,差异无统计学意义（P〉0.05）;②宫颈上皮细胞异常发生情况,不典型鳞状细胞（atypical squamous cell of undetermined signif-icance,ASCUS）、低度鳞状上皮内瘤变（low grade squamous intraepithelial lesion,LSIL）、高度鳞状上皮内瘤变（high grade squamous intraepithelial lesion,HSIL）、鳞癌以及非典型腺细胞的发生率A组分别为12.61%、0.84%、0.36%、0.07 ％、0．29％,组间比较差异无统计学意义（P〉0．05）;③A组60～69岁组宫颈上皮细胞异常的发生率最高（28．57％）,而B组为20～29岁组的发生率最高（15．98％）。结论：内蒙古通辽地区蒙汉族女性宫颈上皮细胞病变发生率相比差异无统计学意义。蒙古族女性宫颈病变患病年龄较汉族女性的患病年龄偏高,液基细胞学检查在我区对提高宫颈癌筛查效率及覆盖率是一种切实、可行的方法。     

药物后适应对急性心肌梗死心肌保护作用的临床研究

目的探讨硝酸甘油和地尔硫卓药物后适应对急性ST段抬高型心肌梗死患者的心肌保护作用。方法比较2003年1月-2007年12月在我中心接受急诊PCI的310例STEMI患者,根据是否给予硝酸甘油＋地尔硫卓药物后适应分为药物后适应组（152例）和对照组（158例）。结果术后即刻药物后适应组cTFC值较低,MBG分级高。药物后适应组的术后12h ST段回落幅度较大,术后5d血清肌酸激酶同工酶曲线下面积较小,心脏超声左心室射血分数较高。另两组随访期间死亡比例和因心脏原因再次住院次数均差异显著。结论在急性ST段抬高型心肌梗死中联合注射硝酸甘油和地尔硫卓进行药物后适应可显著改善再通后心肌的微灌注,减少近期死亡等主要心血管病事件的发生。     

异品系皮肤移植大鼠γδ T细胞培养液与血浆IFN-γ和IL-4水平观察

-4比值均高于对照组;大鼠外周血γδT细胞培养上清IFN-γ、IL-4含量与血浆IFN-γ、IL-4含量分别呈正相关关系.结论 异品系皮肤移植排斥反应发生过程中γγδT细胞能分泌IFN-γ和IL-4,而γδT细胞IFN-γ/IL-4细胞因子的分泌模式可能影响血浆细胞因子格局,从而参与了移植免疫应答过程.     

语前聋儿童人工耳蜗植入术后疗效分析

目的 探索语前聋儿童人工耳蜗植入术后的疗效及相关影响因素.方法 对48例行人工耳蜗植入术的语前聋儿童的家长及康复教师进行问卷调查,调查的内容包括患儿的基本情况、术前评估、植入装置、植入时年龄、术后康复时间、康复模式、术前是否佩戴助听器、言语发展水平满意度及听觉行为分级标准表等.根据听觉行为分级标准(CAP)对患儿听觉感知能力进行评估,并分析病因、性别、病程、康复模式、术前是否佩戴助听器等因素与CAP的相关性.结果 语前聋儿童人工耳蜗植入术后3年能不借助唇读即与人交谈的达到51%,术后5年以上不借助唇读即与人交谈的达到58%.以植入后时间为协变量,采用协方差分析结果显示:病程(P<0.0005)、康复模式(P<0.0005)与CAP显著相关,性别和病因与CAP无关.结论 人工耳蜗植入对语前聋儿童的听觉言语发育有明显的效果.病程限制在2年以内,更有利于人工耳蜗植入儿童的听觉言语康复.对植入人工耳蜗的儿童选择康复机构的康复模式更有利于其听觉言语康复.     

Combi 40+型人工耳蜗植入后电极阻抗的变化规律及其临床意义

目的 研究人工耳蜗植入以后电极阻抗的变化规律及其临床意义.方法 检测多导人工耳蜗系统Combi 40+型人工耳蜗植入患者术中、术后1,2,3,6个月电极阻抗,分析其变化规律及临床意义.结果 植入的Combi 40+型人工耳蜗电极阻抗值在术中最低,开机时最高,以后逐渐下降,并于术后3个月达稳定状态.蜗顶与蜗底处电极阻抗值差异无统计学意义(P > 0.05).结论 耳蜗植入术后,排斥反应使纤维组织包绕电极,在电刺激下,电极阻抗于术后3个月可达稳定状态.检测电极阻抗是目前评估Combi 40+型人工耳蜗植入体状态的有效手段.     

Evaluation of neointimal coverage of overlapping sirolimus- eluting stents by optical coherence tomography

Background Although overlapping sirolimus-eluting stents are often used in long lesions during percutaneous coronary intervention, it was not clear how intimal hyperplasia at the overlapping segments compares with that of single-layer sirolimus-eluting stents.
Methods Optical coherence tomography （OCT） examinations were performed on 22 patients in whom overlapping sirolimus-eluting stents （SESs） were implanted. OCT images were analyzed off-line after the procedure. Still frames were selected and classified, and the length of overlap, lumen loss, and average neointimal thickness on the strut were measured. The stent strut was classified into well-apposed to vessel wall with apparent neointimat coverage （type A）, well-apposed to vessel wall without neointimal coverage （type B）, malapposed to the vessel wall without neointimal coveraae （tvDe C）, and strut located at a major side branch （type D）.
Results Tlqere was no statistically significant difference between strut coverage types within overlapping and non-overlapping segments, but a greater percentage of type C struts were observed within the overlapping segments （5.2% vs 1.4%, P 〉0.05）. Neither neointimal thickness （（175.0±59.9） μm vs （168.3±90.2）μm, P=0.715） nor lumen loss （（1.61±0.55） mm^2 vs （1.48±0.37） mm^2, P=0.397） was statistically different between the two segments. One patient was diagnosed with suspected in-stent thrombosis at 6 months. Although no specific characteristics of thrombosis were seen on the OCT images, a greater number of malapposed struts without neointima coverage were observed.
Conclusions About 90% struts were completely covered by neointimal proliferation at 12 months follow-up, and the thicknesses of neointima on overlapping and non-overlapping segments were similar. Most of type C struts at the overlapping segments were found on the inside layer stents. Delayed antiplatelet therapy was beneficial for the patients with incompletely covered struts.     

A study of association of the complement C4 mutations with systemic lupus erythematosus in the Malaysian population

The aim of the present study was to investigate the association of C4 gene mutations with systemic lupus erythematosus, in 130 Malaysian SLE patients and 130 healthy controls. Generally, various PCR approaches were used to screen the mutations of the C4 genes, which included 2 bp (+TC) insertions at codon 1213 in exon 29, 1 bp deletions (-C) at codon 811 in exon 20, 1 bp (-C), 2 bp (-GT) deletions at codons 522 and 497 in exon 13 and null alleles. No mutations located at exons 13, 20 and 29 of the C4 gene, were detected amongst the patient and control samples in this study. C4A*Q0 was found in two out of the 130 control samples, while C4B*Q0 was present in two out of the 130 SLE patients. Overall, our results do not demonstrate a significant association to these known C4 mutations identified by previous studies, in the Malaysian scenario.