全文获取类型
收费全文 | 1867篇 |
免费 | 203篇 |
国内免费 | 28篇 |
专业分类
耳鼻咽喉 | 7篇 |
儿科学 | 178篇 |
妇产科学 | 53篇 |
基础医学 | 240篇 |
口腔科学 | 90篇 |
临床医学 | 174篇 |
内科学 | 316篇 |
皮肤病学 | 54篇 |
神经病学 | 64篇 |
特种医学 | 200篇 |
外科学 | 348篇 |
综合类 | 56篇 |
预防医学 | 109篇 |
眼科学 | 40篇 |
药学 | 63篇 |
中国医学 | 2篇 |
肿瘤学 | 104篇 |
出版年
2023年 | 14篇 |
2022年 | 10篇 |
2021年 | 19篇 |
2020年 | 17篇 |
2019年 | 25篇 |
2018年 | 52篇 |
2017年 | 50篇 |
2016年 | 45篇 |
2015年 | 66篇 |
2014年 | 83篇 |
2013年 | 124篇 |
2012年 | 62篇 |
2011年 | 43篇 |
2010年 | 88篇 |
2009年 | 105篇 |
2008年 | 52篇 |
2007年 | 67篇 |
2006年 | 61篇 |
2005年 | 42篇 |
2004年 | 44篇 |
2003年 | 28篇 |
2002年 | 31篇 |
2001年 | 33篇 |
2000年 | 24篇 |
1999年 | 35篇 |
1998年 | 106篇 |
1997年 | 87篇 |
1996年 | 99篇 |
1995年 | 64篇 |
1994年 | 75篇 |
1993年 | 41篇 |
1992年 | 22篇 |
1991年 | 39篇 |
1990年 | 21篇 |
1989年 | 42篇 |
1988年 | 32篇 |
1987年 | 38篇 |
1986年 | 35篇 |
1985年 | 28篇 |
1984年 | 14篇 |
1983年 | 9篇 |
1982年 | 20篇 |
1981年 | 16篇 |
1980年 | 19篇 |
1979年 | 8篇 |
1978年 | 9篇 |
1977年 | 11篇 |
1976年 | 16篇 |
1975年 | 12篇 |
1962年 | 2篇 |
排序方式: 共有2098条查询结果,搜索用时 15 毫秒
101.
102.
应用序列特异引物PCR法对广西壮族HLA-DQA_1进行基因分型 总被引:1,自引:0,他引:1
应用序列特异引物多聚酶链反应(PCR)方法对82名无血缘关系的广西壮族健康人的HLA-DQA_1进行基因分型。共检出7种DQA_1等位基因,以DQA_1*0301的频率最高,达31%,其次为DQA_1*0104,0102和0501,检出率分别为24.3%,16.9%和11.7%。未检出的等位基因有DQA_1*0201,0302和0601。结果表明,壮族的HLA-DQA_1等位基因频率分布除与中国南方人相似外,尚有其特点。序列特异引物PCR方法不需要放射性同位素,简便快速、准确可靠的HLA-DQA_1基因分型新方法。 相似文献
103.
104.
Reza Fekrazad Khojasteh Joharchi Katayoun AM Kalhori Fatemeh Mashhadi Abbas Fariborz Salimi Vahid 《Journal of cosmetic and laser therapy》2017,19(4):232-236
Introduction: One of the most important innovative methods for tissue repair promotion is therapeutic lasers with photobiomodulution effects. The aim of this study was to investigate the effect of four different wavelengths of therapeutic laser (405, 532, 660 and 810 nm) on healing of third-degree burns from both clinical and pathological standpoints in rats. Materials and methods: 60 male Wistar rats were used. Animals were anesthetized and dorsal hairs were shaved and third-degree skin burns were created by use of a 95°C copper stamp. Lesions were irradiated with 1.5 J/cm2 energy densities and 200 mW/cm2 power densities. Results: Statistical analyses of the “wound contraction” changes between five groups during the study showed more reduction in wound size in all laser groups in comparison with the control group; but these differences were not statistically significant except between red and blue lasers on the last day of experiment. Discussion: Results of our study showed that using therapeutic lasers with green, blue, red, and infrared wavelengths may accelerate healing process. This trend is more obvious in red and infrared groups especially after acute phase, however, this effect was neither statistically nor clinically significant. 相似文献
105.
106.
107.
108.
109.
M Kurban T Cheng M Wajid M Kiuru Y Shimomura AM Christiano 《Journal of the European Academy of Dermatology and Venereology》2010,24(8):967-969
Background Papillon‐Lefevre syndrome (PLS; OMlM 245000) is an autosomal recessive disease caused by mutations in cathepsin C (CTSC) gene and is characterized by palmoplantar keratoderma, psoriasiform lesion over the extensor surfaces and gingivitis followed by loss of teeth. CTSC gene is expressed in several tissues including the skin and cells of the immune system. In the skin, CTSC plays a role in differentiation and desquamation, whereas in the immune system, it activates serine proteases. Objectives We analysed the molecular basis of PLS in a Pakistani family. Methods Genomic DNA was isolated from the sample according to standard techniques. All exons of the CTSC gene with adjacent sequences of exon–intron borders were amplified by PCR and directly sequenced. Results We identified a novel deletion mutation designated c.2ldelG (Leu7PhefsX57) in exon 1 of the CTSC gene, which probably results in the absence of CTSC protein. Conclusion Our data further expand the spectrum of mutations in the CTSC gene underlying PLS. 相似文献
110.