全文获取类型
收费全文 | 4008327篇 |
免费 | 316257篇 |
国内免费 | 16767篇 |
专业分类
耳鼻咽喉 | 55101篇 |
儿科学 | 128338篇 |
妇产科学 | 106193篇 |
基础医学 | 620326篇 |
口腔科学 | 109173篇 |
临床医学 | 365541篇 |
内科学 | 717737篇 |
皮肤病学 | 99652篇 |
神经病学 | 339222篇 |
特种医学 | 156012篇 |
外国民族医学 | 694篇 |
外科学 | 607741篇 |
综合类 | 118624篇 |
现状与发展 | 23篇 |
一般理论 | 2440篇 |
预防医学 | 340851篇 |
眼科学 | 91805篇 |
药学 | 276965篇 |
27篇 | |
中国医学 | 11547篇 |
肿瘤学 | 193339篇 |
出版年
2021年 | 55872篇 |
2020年 | 35585篇 |
2019年 | 58691篇 |
2018年 | 73295篇 |
2017年 | 56049篇 |
2016年 | 61846篇 |
2015年 | 75380篇 |
2014年 | 109716篇 |
2013年 | 175406篇 |
2012年 | 113027篇 |
2011年 | 117011篇 |
2010年 | 123238篇 |
2009年 | 125384篇 |
2008年 | 102838篇 |
2007年 | 108343篇 |
2006年 | 117460篇 |
2005年 | 112767篇 |
2004年 | 113385篇 |
2003年 | 103714篇 |
2002年 | 93494篇 |
2001年 | 134148篇 |
2000年 | 128815篇 |
1999年 | 122273篇 |
1998年 | 68610篇 |
1997年 | 65626篇 |
1996年 | 63368篇 |
1995年 | 58869篇 |
1994年 | 53043篇 |
1993年 | 49408篇 |
1992年 | 88780篇 |
1991年 | 85297篇 |
1990年 | 81727篇 |
1989年 | 79431篇 |
1988年 | 73863篇 |
1987年 | 72255篇 |
1986年 | 69093篇 |
1985年 | 68112篇 |
1984年 | 58938篇 |
1983年 | 52881篇 |
1982年 | 45350篇 |
1981年 | 42291篇 |
1980年 | 39821篇 |
1979年 | 50396篇 |
1978年 | 42045篇 |
1977年 | 37454篇 |
1976年 | 35182篇 |
1975年 | 34253篇 |
1974年 | 37396篇 |
1973年 | 36025篇 |
1972年 | 33841篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Data on 232 members of a single pedigree, descended from two pairs of original parents, were made available to the participants of Genetic Analysis Workshop 8 (GAW8). In addition to information concerning age and sex, measurements for 10 quantitative traits and genotypes at 22 polymorphic marker loci were also provided for a subset of 193 of these family members. © 1993 Wiley-Liss, Inc. 相似文献
992.
A E van der Merwe F J Mattheyse M Bedford P D van Helden D J Rossouw 《Burns : journal of the International Society for Burn Injuries》1990,16(3):193-197
Cultured keratinocytes were used as allografts on burn wounds in two patients. In both patients successful covering of the wounds was obtained. DNA fingerprinting of the epidermis covering the wounds 21 days later showed that the cultured keratinocytes were replaced by the patients' cells. 相似文献
993.
Primary extraosseous osteogenic sarcoma of the mediastinum: clinical, pathologic, and radiologic correlation 总被引:1,自引:0,他引:1
R W Tarr T Kerner B McCook D L Page E P Nance J J Kaye 《Southern medical journal》1988,81(10):1317-1319
We have presented a rare case of primary involvement of the anterior mediastinum by osteogenic sarcoma in which CT was useful in demonstrating calcium within the mass, and in distinguishing the mass from surrounding bony structures. Although rare, extraosseous osteogenic sarcoma should be included in the differential diagnosis of a calcified anterior mediastinal mass. 相似文献
994.
Rapid reorganization of adult rat motor cortex somatic representation patterns after motor nerve injury. 总被引:2,自引:0,他引:2 下载免费PDF全文
J N Sanes S Suner J F Lando J P Donoghue 《Proceedings of the National Academy of Sciences of the United States of America》1988,85(6):2003-2007
The potential for peripheral nerve injury to reorganize motor cortical representations was investigated in adult rats. Maps reflecting functional connections between the motor cortex and somatic musculature were generated with intracortical electrical stimulation techniques. Comparison of cortical somatotopic maps obtained in normal rats with maps generated from rats with a facial nerve lesion indicated that the forelimb and eye/eyelid representations expanded into the normal vibrissa area. Repeated testing from an electrode placed chronically in the motor cortex showed a shift from vibrissa to forelimb within hours after facial nerve transection. These comparatively quick changes in motor cortex representation pattern suggest that synaptic relations between motor cortex and somatic musculature are continually reshaped in adult mammals. 相似文献
995.
CT- or sonography-guided biopsy of the liver in the presence of ascites: frequency of complications 总被引:1,自引:0,他引:1
F B Murphy K P Barefield H V Steinberg M E Bernardino 《AJR. American journal of roentgenology》1988,151(3):485-486
The presence of ascites has been considered a contraindication to percutaneous biopsy of the liver. To determine the validity of this assumption, we performed percutaneous biopsies of the liver under CT or sonographic guidance in 28 patients who had ascites and in 28 patients who did not have ascites and compared the complication rates in the two groups. Twenty-two patients (79%) in the group with ascites and 19 patients (68%) in the group without ascites had biopsies to determine the cause or extent of chronic liver disease. The remainder were oncologic patients who had biopsies to determine the cause of a focal hepatic mass. The complication rate in the patients who had ascites (32%) was less than that in the patients who did not have ascites (43%) (the difference did not reach statistical significance, p less than .30). In the ascites group, complications included transient hypotension (five patients), a mild-to-moderate fall in hematocrit (three patients), and a small leak of ascites from the biopsy site (one patient). In the control group, minor complications included transient hypotension (three patients), a mild-to-moderate fall in hematocrit (seven patients), and a small subcapsular hematoma (one patient). One major complication occurred in the control group: a patient required a blood transfusion because of the fall in his hematocrit. We conclude that the complication rate in liver biopsies guided by CT or sonography in the presence of ascites is not higher than similar biopsies done in the absence of ascites. Ascites should not be considered a contraindication for performing such biopsies. 相似文献
996.
J Combe P Poinsard J Besancenot G Camelot F Cattin J F Bonneville T Moulin J L Henlin J L Chopard L Cotte 《Annals of vascular surgery》1990,4(6):558-562
Free-floating clots of the extracranial internal carotid artery are generally considered as surgical emergencies. This retrospective study analyzes six free-floating clots diagnosed by arteriography. Three of these patients had a fixed stroke while the other three had an evolving stroke. Three patients had antecedent ocular or hemispheric transient ischemic attacks. The causes of free-floating clots in the internal carotid artery were atheromatous stenosis in two cases, ulcerated plaque in three cases, and carotid artery dissection in one. All six patients were seen late, approximately 15 hours after their neurologic accident. They were treated with intravenous heparin over a two to five week period. Repeat arteriograms demonstrated complete clot lysis in four instances, while partial lysis was seen in one case. Moderate extension of thrombus occurred in one case only. No further neurologic complications were noted during the treatment by heparin. As indicated by follow-up arteriographic findings, secondary surgery was performed for major carotid lesions and residual clots in five cases. The free-floating thrombus syndrome of the carotid artery should not be considered as a surgical emergency when discovered late in the wake of an acute neurologic accident. 相似文献
997.
A Espinosa de los Monteros G Roussel N M Neskovic J L Nussbaum 《Journal of neuroscience research》1988,19(2):202-211
A new chemically defined medium consisting of equal parts of Dulbecco modified Eagle's and Ham's F-12 media supplemented with insulin, sodium selenite, putrescine, and D+ galactose, which allows the long-term survival of mature oligodendrocyte pure cultures, is described. Immunohistochemical staining has shown that over 90% of the cells become positive for myelin proteins shortly following subculture. Contaminating astrocytes (2%) do not survive in this medium. Biochemical data have indicated that these purified oligodendrocytes express 2'3'-cyclic nucleotide 3'-phosphohydrolase and UDP-galactose ceramide galactosyltransferase activities. Electron microscopical examination revealed that the oligodendrocytes were mostly of medium-dark type and appeared to be identical to cells cultured in serum-containing medium. The ability to maintain pure oligodendrocyte cultures in such a defined medium will allow investigations concerning exogenous and endogenous factors involved in oligodendrocyte metabolism. 相似文献
998.
S K Malhotra R Predy R Singh B L Gupta R Bhatnagar K Leeuw 《Journal of neuroscience research》1988,19(4):420-7, 472-3
A mouse monoclonal antibody (MAb 6B9, isotype IgM) was raised against autopsy tissue samples from the central nervous system (CNS) of multiple sclerosis (MS) patients. By immunofluorescence microscopy, MAb 6B9 intensely stains most or all cells in fetal rats. However, MAb 6B9 differentially stains various cell types in adult rats. Neurons, ependymal cells, and adrenal chromaffin cells are stained intensely, whereas astrocytes and oligodendrocytes are not stained. The 6B9-reactive antigen (6B9 antigen) is sensitive to periodic acid, but insensitive to treatment with protease, RNase, or hyaluronidase. Results from immunofluorescence microscopy on semithin sections and cultured neuroblastoma cells indicate that 6B9 antigen is intracellular. This is supported by immunoelectron microscopy, where labeling for 6B9 antigen appears in the cytoplasm distinct from any identifiable organelle. Further studies on 6B9 antigen should reveal its chemical nature as well as the significance of developmental changes in its distribution. 相似文献
999.
Facilitated uptake of zinc into human erythrocytes. Relevance to the treatment of sickle-cell anaemia 总被引:1,自引:0,他引:1
R C Hider L Ejim P D Taylor R Gale E Huehns J B Porter 《Biochemical pharmacology》1990,39(6):1005-1012
The ability of a number of heterocyclic metal chelators to deliver zinc into red cells, to release the liganded zinc to haemoglobin and thereby cause a left shift in the oxygen dissociation curve of intact red cells has been investigated. Incubation of neutrally charged zinc-pyrone and zinc-pyridin-2-one complexes with red cells led to the rapid accumulation of zinc within cells, whereas unliganded zinc in the form of zinc acetate, zinc chloride or zinc sulphate accumulated only slowly. The rate at which zinc was delivered to red cells by pyrone and pyridin-2-one ligands increased with increasing lipid solubility of the ligands. The uptake of zinc into both normal adult and sickle red cells was associated with a dose-dependent increase in the oxygen affinity of haemoglobin. The degree of left shift in the oxygen dissociation curve following the incubation of red cells with zinc-pyrone and -pyridin-2-one complexes suggests that these complexes may find application as agents to increase the oxygen affinity of haemoglobin in sickle cell disease and thereby decrease the probability of intravascular sickling at low tissue oxygen tensions. Ethylmaltol appears to be a particularly useful agent due to its known low toxicity. 相似文献
1000.
We have studied the effects of semi-synthetic diets rich in either n-9 (olive oil, OO) or n-6 (corn oil, CO), or n-3 (fish oil, FO, as MaxEPA) fatty acids on the levels of major PUFA in platelet lipids, on the generation of inositol phosphates by [3H]inositol labelled platelets after stimulation with thrombin and of thromboxane B2 (TxB2) by platelet rich plasma (PRP) after stimulation with collagen. The predicted elevations of oleic (OA), linoleic (LA) and eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were observed in platelet lipids of each animal group, but in the MaxEPA fed group accumulation of EPA was associated with depletion of linoleic acid (LA) rather than of arachidonic acid (AA). Basal levels of inositol-tris-phosphate (IP3) in platelets were lowest in the OO group and highest in the CO group, whereas the increment after thrombin stimulation (1 unit/ml NIH) was maximal in the OO group and minimal in the FO group. Instead, when generation of TxB2 by stimulated platelets was evaluated, no appreciable difference among the various groups could be detected, in accordance with the limited modifications of platelet AA content induced by the diets. The overall data indicate that dietary fatty acids modulate the pathway of inositol phosphate generation in rabbit platelets, independently of modifications of TxB2 production. 相似文献