全文获取类型
收费全文 | 2341543篇 |
免费 | 174720篇 |
国内免费 | 5779篇 |
专业分类
耳鼻咽喉 | 32180篇 |
儿科学 | 77571篇 |
妇产科学 | 66865篇 |
基础医学 | 343615篇 |
口腔科学 | 65372篇 |
临床医学 | 215728篇 |
内科学 | 451913篇 |
皮肤病学 | 47881篇 |
神经病学 | 189547篇 |
特种医学 | 87805篇 |
外国民族医学 | 696篇 |
外科学 | 342142篇 |
综合类 | 51068篇 |
现状与发展 | 2篇 |
一般理论 | 729篇 |
预防医学 | 188473篇 |
眼科学 | 53210篇 |
药学 | 175990篇 |
7篇 | |
中国医学 | 5044篇 |
肿瘤学 | 126204篇 |
出版年
2018年 | 25209篇 |
2017年 | 18973篇 |
2016年 | 20978篇 |
2015年 | 24037篇 |
2014年 | 33179篇 |
2013年 | 51216篇 |
2012年 | 70243篇 |
2011年 | 74947篇 |
2010年 | 43944篇 |
2009年 | 40837篇 |
2008年 | 70249篇 |
2007年 | 74990篇 |
2006年 | 75790篇 |
2005年 | 73755篇 |
2004年 | 70574篇 |
2003年 | 67721篇 |
2002年 | 66181篇 |
2001年 | 103820篇 |
2000年 | 106501篇 |
1999年 | 90072篇 |
1998年 | 25964篇 |
1997年 | 23422篇 |
1996年 | 23363篇 |
1995年 | 22108篇 |
1994年 | 20839篇 |
1993年 | 19439篇 |
1992年 | 72428篇 |
1991年 | 71351篇 |
1990年 | 69691篇 |
1989年 | 67041篇 |
1988年 | 62267篇 |
1987年 | 60819篇 |
1986年 | 58140篇 |
1985年 | 55618篇 |
1984年 | 41779篇 |
1983年 | 35929篇 |
1982年 | 21685篇 |
1981年 | 19228篇 |
1979年 | 39017篇 |
1978年 | 27646篇 |
1977年 | 23317篇 |
1976年 | 22466篇 |
1975年 | 23854篇 |
1974年 | 28556篇 |
1973年 | 27843篇 |
1972年 | 26144篇 |
1971年 | 24262篇 |
1970年 | 22596篇 |
1969年 | 21278篇 |
1968年 | 20101篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
T Uno H Kondo Y Inoue Y Kawahata M Sotomura K Iuchi G Tsukamoto 《Journal of medicinal chemistry》1990,33(10):2929-2932
A series of novel pyridone carboxylic acids having a 4-hydroxypiperazinyl group at the 7-position of norfloxacin and ciprofloxacin were prepared. The in vivo antibacterial efficacies of these compounds were superior to those of corresponding piperazinyl derivatives. From the results of the studies on the pharmacokinetic profile and toxicity, the 4-hydroxypiperazinyl derivatives were confirmed to be pharmacologically superior to corresponding piperazinyl derivatives. Thus, a 4-hydroxypiperazinyl group was revealed to be a beneficial substituent for potential use in future quinolone antibacterials. 相似文献
992.
J Combe P Poinsard J Besancenot G Camelot F Cattin J F Bonneville T Moulin J L Henlin J L Chopard L Cotte 《Annals of vascular surgery》1990,4(6):558-562
Free-floating clots of the extracranial internal carotid artery are generally considered as surgical emergencies. This retrospective study analyzes six free-floating clots diagnosed by arteriography. Three of these patients had a fixed stroke while the other three had an evolving stroke. Three patients had antecedent ocular or hemispheric transient ischemic attacks. The causes of free-floating clots in the internal carotid artery were atheromatous stenosis in two cases, ulcerated plaque in three cases, and carotid artery dissection in one. All six patients were seen late, approximately 15 hours after their neurologic accident. They were treated with intravenous heparin over a two to five week period. Repeat arteriograms demonstrated complete clot lysis in four instances, while partial lysis was seen in one case. Moderate extension of thrombus occurred in one case only. No further neurologic complications were noted during the treatment by heparin. As indicated by follow-up arteriographic findings, secondary surgery was performed for major carotid lesions and residual clots in five cases. The free-floating thrombus syndrome of the carotid artery should not be considered as a surgical emergency when discovered late in the wake of an acute neurologic accident. 相似文献
993.
A Espinosa de los Monteros G Roussel N M Neskovic J L Nussbaum 《Journal of neuroscience research》1988,19(2):202-211
A new chemically defined medium consisting of equal parts of Dulbecco modified Eagle's and Ham's F-12 media supplemented with insulin, sodium selenite, putrescine, and D+ galactose, which allows the long-term survival of mature oligodendrocyte pure cultures, is described. Immunohistochemical staining has shown that over 90% of the cells become positive for myelin proteins shortly following subculture. Contaminating astrocytes (2%) do not survive in this medium. Biochemical data have indicated that these purified oligodendrocytes express 2'3'-cyclic nucleotide 3'-phosphohydrolase and UDP-galactose ceramide galactosyltransferase activities. Electron microscopical examination revealed that the oligodendrocytes were mostly of medium-dark type and appeared to be identical to cells cultured in serum-containing medium. The ability to maintain pure oligodendrocyte cultures in such a defined medium will allow investigations concerning exogenous and endogenous factors involved in oligodendrocyte metabolism. 相似文献
994.
S K Malhotra R Predy R Singh B L Gupta R Bhatnagar K Leeuw 《Journal of neuroscience research》1988,19(4):420-7, 472-3
A mouse monoclonal antibody (MAb 6B9, isotype IgM) was raised against autopsy tissue samples from the central nervous system (CNS) of multiple sclerosis (MS) patients. By immunofluorescence microscopy, MAb 6B9 intensely stains most or all cells in fetal rats. However, MAb 6B9 differentially stains various cell types in adult rats. Neurons, ependymal cells, and adrenal chromaffin cells are stained intensely, whereas astrocytes and oligodendrocytes are not stained. The 6B9-reactive antigen (6B9 antigen) is sensitive to periodic acid, but insensitive to treatment with protease, RNase, or hyaluronidase. Results from immunofluorescence microscopy on semithin sections and cultured neuroblastoma cells indicate that 6B9 antigen is intracellular. This is supported by immunoelectron microscopy, where labeling for 6B9 antigen appears in the cytoplasm distinct from any identifiable organelle. Further studies on 6B9 antigen should reveal its chemical nature as well as the significance of developmental changes in its distribution. 相似文献
995.
Facilitated uptake of zinc into human erythrocytes. Relevance to the treatment of sickle-cell anaemia 总被引:1,自引:0,他引:1
R C Hider L Ejim P D Taylor R Gale E Huehns J B Porter 《Biochemical pharmacology》1990,39(6):1005-1012
The ability of a number of heterocyclic metal chelators to deliver zinc into red cells, to release the liganded zinc to haemoglobin and thereby cause a left shift in the oxygen dissociation curve of intact red cells has been investigated. Incubation of neutrally charged zinc-pyrone and zinc-pyridin-2-one complexes with red cells led to the rapid accumulation of zinc within cells, whereas unliganded zinc in the form of zinc acetate, zinc chloride or zinc sulphate accumulated only slowly. The rate at which zinc was delivered to red cells by pyrone and pyridin-2-one ligands increased with increasing lipid solubility of the ligands. The uptake of zinc into both normal adult and sickle red cells was associated with a dose-dependent increase in the oxygen affinity of haemoglobin. The degree of left shift in the oxygen dissociation curve following the incubation of red cells with zinc-pyrone and -pyridin-2-one complexes suggests that these complexes may find application as agents to increase the oxygen affinity of haemoglobin in sickle cell disease and thereby decrease the probability of intravascular sickling at low tissue oxygen tensions. Ethylmaltol appears to be a particularly useful agent due to its known low toxicity. 相似文献
996.
We have studied the effects of semi-synthetic diets rich in either n-9 (olive oil, OO) or n-6 (corn oil, CO), or n-3 (fish oil, FO, as MaxEPA) fatty acids on the levels of major PUFA in platelet lipids, on the generation of inositol phosphates by [3H]inositol labelled platelets after stimulation with thrombin and of thromboxane B2 (TxB2) by platelet rich plasma (PRP) after stimulation with collagen. The predicted elevations of oleic (OA), linoleic (LA) and eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were observed in platelet lipids of each animal group, but in the MaxEPA fed group accumulation of EPA was associated with depletion of linoleic acid (LA) rather than of arachidonic acid (AA). Basal levels of inositol-tris-phosphate (IP3) in platelets were lowest in the OO group and highest in the CO group, whereas the increment after thrombin stimulation (1 unit/ml NIH) was maximal in the OO group and minimal in the FO group. Instead, when generation of TxB2 by stimulated platelets was evaluated, no appreciable difference among the various groups could be detected, in accordance with the limited modifications of platelet AA content induced by the diets. The overall data indicate that dietary fatty acids modulate the pathway of inositol phosphate generation in rabbit platelets, independently of modifications of TxB2 production. 相似文献
997.
Reduction of reticulata neuronal activity by zolpidem and alpidem, two imidazopyridines with high affinity for type I benzodiazepine receptors 总被引:1,自引:0,他引:1
G Mereu G Carcangiu A Concas N Passino G Biggio 《European journal of pharmacology》1990,179(3):339-345
Zolpidem and alpidem, two imidazopyridines with high affinity for the type I benzodiazepine recognition site, have recently been proposed as preferential hypnotic (zolpidem) and anxiolytic (alpidem) drugs notable for the minor incidence of side-effects. To further characterize the molecular mechanism involved in the action of these drugs, we studied their effects in comparison with those of diazepam on the spontaneous electrical activity of substantia nigra pars reticulata (SNR) neurons. These cells have been shown to be extremely sensitive to various positive and negative modulators of GABAergic transmission. All three drugs consistently produced a dose-dependent (0.03-8.0 mg/kg i.v.) inhibition of the firing of SNR cells when administered as a single bolus. However, zolpidem was more potent and efficacious than diazepam or alpidem. The ID50s were 0.076, 0.492 and 0.821 mg/kg, respectively. When the drugs were injected in exponentially (ratio 2) increasing doses up to 8.0 mg/kg, the rank order for tachyphylaxis was zolpidem much greater than diazepam greater than alpidem. Since the effects of the drugs were abolished and prevented by a small dose (0.5 mg/kg i.v.) of flumazenil (Ro 15-1788), it is likely that the effects were mediated through activation of benzodiazepine receptors. The results indicate that the hypnotic, zolpidem, has a more potent inhibitory action on SNR cell activity than the anxiolytics, alpidem and diazepam. 相似文献
998.
The present study reports the differential effects of pertussis toxin on muscarinic regulation of intracellular Ca2+ and inositol phosphate generation and alpha 2-adrenoceptor-mediated inhibition of cAMP formation in SH-SY5Y human neuroblastoma cells. Carbachol caused a biphasic increase in intracellular Ca2+ (release of internal stores and Ca2+ entry) and a dose-dependent increase in inositol phosphate formation. Pertussis toxin pretreatment did not affect either of these components of the signal transduction pathway but did completely reverse the alpha 2-adrenoceptor-mediated inhibition of forskolin-stimulated cAMP formation. These data indicate that muscarinic regulation of inositol phosphate generation occurs via a pertussis toxin-insensitive G-protein and that Ca2+ entry in these cells may not occur via a G-protein. 相似文献
999.
The amount of endothelial cell loss that occurs to the donor cornea during the trephination process was evaluated in 40 porcine eyes. A vital staining technique (alizarin red S and acid violet 19) was used to quantify the extent of endothelial damage and removal. Two types of corneal punches (Weck and H&I0 were used for trephination. The average amount of endothelial loss from the peripheral cornea was 7.9%, accounting for only a 3% loss of endothelial cells over the total surface area of the corneal button. a comparison of the Weck and H&I corneal punches showed no significant difference (P greater than 0.1) by Student's test for both endothelial cell damage and removal. 相似文献
1000.
The effect of lysozyme-inactivation on L(+)-lactic acid (LA) production in dental plaque suspensions was evaluated. From 10 children 24-h plaque was collected and lysozyme activity inhibited by addition of goat antiserum to human lysozyme. Acid production was stimulated by addition of glucose. The results showed significantly increased LA levels (50-150%) in lysozyme-inactivated plaque suspensions from 8 of the subjects compared to untreated controls. The increase in acid production activity was not related to plaque lysozyme levels. The findings indicate that the presence of lysozyme may be limiting on acid production in the early dental plaque. 相似文献