多巴酚丁胺负荷实验对家猪急性冬眠心肌模型QT离散度影响的实验研究

目的 观察多巴酚丁胺负荷期间QT离散度(QTd)的变化情况,探讨其临床意义.方法 对13只小型中国家猪采用闭胸介入法制备急性冬眠心肌模型(右冠状动脉为靶血管),利用多巴酚丁胺超声负荷实验(DSE,0～40 μg·Kg-1·min-1)检出冬眠心肌,之后成功复灌,处死动物,对心脏行TTC大体染色及光镜检查.分别于模型制备前后、DSE期间及复灌后观察QTd的变化情况.结果 ①10只动物(76.92%)成功制备成模型,病理检查未示心肌坏死改变.②模型制备成功后QTd较正常状态明显增加[(68.75±5.33)ms和(25.00±1.77)ms,P＜0.05];复灌后明显降低[(35.62±3.47)ms和(68.75±5.33)ms,P＜0.05],与正常状态差异无统计学意义[(35.62±3.47)ms和(25.00±1.77)ms,P＞0.05].③DSE期间QTd呈现先降低后增加的双相反应的变化趋势.④模型制备成功后的QTd与DSE检出的急性冬眠心肌节段数呈中等程度的负相关(r=-0.64,P＜0.05).结论 ①.急性冬眠心肌动物模型的QTd明显增加,早期有效复灌可降低QTd.②DSE期间急性冬眠心肌模型的QTd呈现双相反应趋势.③急性冬眠心肌模型的QTd是反映冬眠心肌数量的指标之一.     

鸡抗内毒素卵黄抗体IgY的制备

目的：研究分别应用内毒素（LPS）、类脂A(LipidA)和大肠杆菌突变株15免疫鸡后其蛋黄中抗内毒素抗体IgY的产量、纯度、效价并筛选最佳免疫抗原。方法：分别应用内毒素（LPS）、类脂A(LipidA)和大肠杆菌J5突变株作为抗原免疫25周龄Leghom鸡，水溶法（WD）提取蛋黄中抗体IgY，双紫外光测定抗体含量，SDS-PSGE电泳检测抗体纯度，细胞酶联染色和ELISA检测抗体特异性、效价及筛选最佳免疫抗原。结果：3种抗内毒素IgY含量和效价分别为14.4mg/ml和1：12 800（J5）、10.61mg/ml和1：12 800（LPS）、9.26mg/ml和1：3200（LipidA），抗体纯度均为95％左右。结论：大肠杆菌突变株J5和内毒素（LPS）为最佳免疫抗原，免疫鸡后其蛋黄中抗内毒素抗体IgY的产量和效价最高。     

9例结石性肠梗阻的诊断与治疗

目的 探讨结石性肠梗阻的诊断及治疗。方法 比较腹部平片和B超的术前诊断率。随访患者以了解不同手术方法的得失。结果 腹部平片术前诊断率为22％,辅以B超则上升为66％。4例Ⅰ期手术和3例Ⅱ期手术患者无死亡。无胆管炎发作。结论 仅靠腹部平片易漏诊,辅以B超则能提高术前诊断率。争取回期手术切除胆囊、闭合胆肠瘘,如有困难,则动员患者6-8周后Ⅱ期手术。     

A Clinical Study of Reversing Left Ventricular Hypertrophy in Hypertensive Patients by Adalat

ＡＣｌｉｎｉｃａｌＳｔｕｄｙｏｆＲｅｖｅｒｓｉｎｇＬｅｆｔＶｅｎｔｒｉｃｕｌａｒＨｙｐｅｒｔｒｏｐｈｙｉｎＨｙｐｅｒｔｅｎｓｉｖｅＰａｔｉｅｎｔｓｂｙＡｄａｌａｔＺｈａｎｇＦｕｍｉｎ（张馥敏）ＸｕＤｉ（许迪）ＹｏｎｇＹｏｎｇｈｏｎｇ（雍永宏）Ｃｈｅｎ...     

Shape changes produced in detergent extracted bovine retinal pigment epithelium when exposed to ATP. A comparison with fibroblasts and smooth muscle cells

The response of single detergent treated bovine retinal pigment epithelial cells in culture to ATP was measured with an image analyser. The most pronounced contraction was produced by 1.0 mM ATP with most change taking place in the first 10 min. At 1 h the area had decreased by about 33%, perimeter 22% and maximum length 25%. By way of comparison rabbit skin fibroblasts had a decreased area of approximately 40%, perimeter 25% and maximum length 22%. Bovine aortic smooth muscle cells on the other hand decreased in area by 55%, perimeter 40% and maximum length 36%. It is hoped that this assay may be used to evaluate drugs which could counteract contractile events in proliferative vitreoretinopathy.     

Traumatic infarction of the spinal cord in children

Infarction of the spinal cord in childhood is rarely due to trauma. During a 15-year period (1971 to 1985), eight children were admitted to The Hospital for Sick Children, Toronto, with a diagnosis of traumatic infarction of the spinal cord. All of these patients had delayed onset of neurological signs varying between 2 hours and 4 days after their initial trauma. No bone abnormalities were seen on plain spine x-ray films. Myelography was carried out in seven of these children and found to be normal in all seven. Six patients who were paraplegic at the time of admission remained permanently paraplegic, but two with incomplete cord signs did show some improvement.     

先天性胆总管囊肿23例的术式及疗效分析

目的：探讨先天性胆总管囊肿的术式及疗效。方法：回顾分析手术治疗先天性胆总管囊肿23例，其中Roux-Y囊肿-空肠吻合8例；囊肿切除，Roux-Y肝总管-空肠吻合15例。结果：20例获得随访，Roux-Y囊肿-空肠吻合随访6例，仅2例无症状，另4例反复发作腹痛，发热或黄疸，其中1例再手术，囊肿切除，Roux-Y肝总管-空肠吻合随访14例。仅1例有腹痛，余13例无症状，结论：Roux-Y肝总管-空肠吻合是治疗先天性胆总管囊肿的首选术式。     

Changes in outcome with sphincter preserving surgery for rectal cancer in Korea, 1991-2000.

AIM: To report the clinical and oncological data of patients operated on for rectal cancers 3-5 cm from the AV over a 10 year period, including the Sphincter preservation (SP) rate. METHODS: We reviewed medical records of 304 patients with rectal cancers 3-5 cm from the AV who underwent surgical resection from January 1991 through December 2000. The 10 years were divided into three periods based on the introduction of new surgical techniques, specifically, ultralow anterior resection (ULAR) with double stapling in March 1994 and ULAR with coloanal anastomosis in April 1997. The rates of SP, complications and patient survival during these periods were compared. RESULTS: The SP rate increased significantly over the 10 years, from 16.4% in period I (January 1991-February 1994), to 53.0% in period II (March 1994-March 1997), to 86.5% in period III (April 1997-December 2000) (p<0.001). Over time, the age of the patients increased (p=0.004), the length of the distal resection margin became shorter (p=0.005), and the rate of lymph node metastasis increased (p=0.016). The factors significantly influencing SP were the period (p<0.001) and the distance from the AV (p<0.001). Over time, morbidity did not increase, and overall and disease free survival rates did not decrease. In contrast, the overall survival of N2 cases significantly increased over time (p=0.0492). CONCLUSION: Over 10 years, the SP rate in rectal cancers 3-5 cm from the AV was significantly increased by the introduction of the double stapling and coloanal anastomosis techniques. These surgical methods, however, had no effect on morbidity, disease free survival and overall survival rates.