Hepatic transplantation from living relative donor

Modern approaches for hepatic transplantation in adults and children are enlighten. Analysis of 34 transplantation operations of hepatic part from living relative donor was conducted. Data on need for hepatic transplantation performance are adduced, indications for its conduction are determined. There are also suggested the main methods of hepatic transplantation from living relative donor, together with immunosuppression issues, and the justice and deontological problem aspects.     

Effect of spironolactone on hypertrophy of left ventricular myocardium in Wistar rats with experimental uremia

Chronic renal failure was modeled in rats by partial nephrectomy. Blood pressure, heart rate, concentrations of aldosterone, urea, creatinine, electrolytes, and protein, index of hypertrophy of visceral organs, and 24-h diuresis were evaluated. In rats treated with spironolactone, the index of myocardial hypertrophy did not considerably differ from that in sham-operated animals, whereas in untreated rats the test parameters considerably differ from the control. We concluded that the blockade of aldosterone receptors with spironolactone produced a cardioprotective effect in Wistar rats with subtotal nephrectomy. __________ Translated from Byulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 145, No. 6, pp. 659–662, June, 2008     

Cloning, expression and initial characterization of interleukin-19 (IL-19), a novel homologue of human interleukin-10 (IL-10)

Interleukin-10 (IL-10) is a pleiotropic cytokine with important immunoregulatory functions whose actions influence activities of many of the cell-types in the immune system. We report here identification and cloning of a gene and corresponding cDNAs encoding a novel homologue of IL-10, designated IL-19. IL-19 shares 21% amino acid identity with IL-10. The exon/intron structure of IL-19 is similar to that of the human IL-10 gene, comprising five exons and four introns within the coding region of the IL-19 cDNA. There are at least two distinct IL-19 mRNA species that differ in their 5'-sequences, suggesting the existence of an intron in the 5'-sequences of coding portion of the IL-19 gene. The longer 5'-sequence contains an alternative initiating ATG codon that is in-frame with the rest of the coding sequence. The expression of IL-19 mRNA can be induced in monocytes by LPS-treatment. The appearance of IL-19 mRNA in LPS-stimulated monocytes was slightly delayed compared to expression of IL-10 mRNA: significant levels of IL-10 mRNA were detectable at 2 h post-stimulation, whereas IL-19 mRNA was not detectable until 4 h. Treatment of monocytes with IL-4 or IL-13 did not induce de novo expression of IL-19, but these cytokines did potentiate IL-19 gene expression in LPS-stimulated monocytes. In addition, GM-CSF was capable of directly inducing IL-19 gene expression in monocytes. IL-19 does not bind or signal through the canonical IL-10 receptor complex, suggesting existence of an IL-19 specific receptor complex, the identity of which remains to be discovered.