腺病毒介导的大肠杆菌CD自杀基因体内外对肿瘤杀伤作用的研究

编委会 Editorial Board名誉主编 Honorary Editor-in-chief吴孟超 Wu Meng chao(Second Military Medical University,Shanghai 200433学术顾问 Academic Advisers巴德年 Ba Denian(Chinese Academy of Medical Sciences,Beijing 100730)刘新垣 Liu Xinyuan(Shanghai Institute of Biochemistry,Chinese Academy of Sciences,Shanghai 200031)吴旻 WuMin(Cancer Institute,Chinese Academy of Medical Sciences,Beijing 100021)汤钊猷 Tang Zhaoyou(Shanghai Medical University,Shanghai 200032)主编 Editor-in-chief张友会 Zhang Youhui(Cancer Institute,Chinese Academy of Medical Sciences,Beijing,100021)副主编 Associate Editor-in-chief崔正言 Cul Zhenyan(Department of Immunology,Shandong Academy of Medical Sciences,Jinan 250001)钱振超 Qian Zhenchao(Department of Patho-physiology,Dalian Medical University,Dalian 116027)何球藻 He Qiuzao(Department of Immunology,Shanghai Medical University,Shanghai 200032)董志伟 Dong Zhiwei(Cancer Institute,Chinese Academy of Medical Sciences,Beijing 100021)常务副主编 Managing Editor-in-chief     

RELATIONSHIP OF THE EXPRESSION OF MDR1 GENE PRODUCT IN HEPATO CELLULAR CARCINOMA TO INVASION AND METASTASIS

ＩＮＴＲＯＤＵＣＴＩＯＮＰｇｌｙｃｏｐｒｏｔｅｉｎ（Ｐｇｐ），ｔｈｅｐｒｏｄｕｃｔｏｆｔｈｅｍｕｌｔｉｄｒｕｇｒｅｓｉｓｔａｎｃｅ（ＭＤＲ１）ｇｅｎｅ，ｉｓａ１７０Ｋｄａｍｅｍｂｒａｎｅｐｒｏｔｅｉｎｒｅｓｐｏｎｓｉｂｌｅｆｏｒｐｕｍｐｉｎｇ...     

河套大黄非蒽醌类成分研究

从河套大黄干燥根与根茎的甲醇提取物中分得8个非蒽醌类成分。经化学和波谱方法，确定它们的结构分别为：β-谷甾醇（β-sitosterol，Ⅰ）、土大黄苷元（rhapontigeni，Ⅱ）、没食子酸（gallicacid，Ⅲ）、3－（3’，5’-二羟基-反式-肉桂酰基）－5-羟基-△5－α-吡喃酮[3－（3’，5’－dihydroxyl－trans－cinnamoyl）－5－hydroxyl－△5’－α－pyranone，Ⅳ]、胡萝卜苷（daucosterol，Ⅴ）、piceatannol－3’－O-β-D-glucopyranoside（Ⅵ）、土大黄苷（rhaponticin，Ⅶ）和蔗糖（Ⅷ）。其中Ⅳ为一新化合物，命名为波叶素（rheumin）。Ⅰ、Ⅲ、Ⅴ、Ⅵ和Ⅷ系首次从该植物中获得。     

危北海脾胃病学术思想探讨

危北海教授学术思想是建立在对脾胃疾患的长期研究和临床实践基础之上的.他认为脾胃虚证是脾胃病研究的关键所在,脾胃疾病的基本发病机制是脾胃内伤所致.为此,危先生从脾脏本脏及基与他脏的关系方面探讨脾胃虚弱在慢性胃肠病发病机制和诊治中的意义,把脾脏本脏及脾脏与他脏的关系体现在用药上,在临床上注意辨证相结合,并详辨脾阴虚与胃阴虚、胃气虚与脾气虚的不同,注重脾胃病的详辨与详审,作到灵活用药.     

云南玉溪地区甲型副伤寒沙门菌的耐药性变迁

目的了解本地区甲型副伤寒沙门菌对几种临床常用药物的耐药现状,重点讨论培氟沙星的耐药性变迁。方法就本院1999年1月至2004年8月从患者临床标本中分离的2335株甲型副伤寒沙门菌,对几种临床常用抗菌药物的药敏试验结果作动态分析。结果甲型副伤寒沙门菌对培氟沙星的耐药率已由1999年的0上升到2004年的98.1%(P<0.01),MIC90≥4mg·L-1;头孢噻肟、头孢他啶、头孢西丁、亚胺培南、呋喃妥因以及复方磺胺甲口恶唑的耐药率均相对较低,MIC90分别为≤4、8、4、4、32和10mg·L-1。结论甲型副伤寒沙门菌对培氟沙星的耐药率上升迅速;临床需加强对氟喹诺酮类药物的合理使用。     

正交试验法优化硫酸多黏菌素E脂质体的处方

目的 采用正交设计法筛选硫酸多黏菌素E脂质体的最优处方，建立透析法作为测定脂质体包封率的方法。方法 逆相蒸发制备脂质体，紫外分光光度法测定硫酸多黏菌素E含量，正交设计法优化硫酸多黏菌素E脂质体处方。结果 采用正交设计法筛选出的脂质体粒径均匀、外观较好，包封率可达50％。结论 采用正交设计法可以方便的筛选出硫酸多黏菌素E脂质体的最优处方。采用透析法测定硫酸多黏菌素E脂质体的包封率准确性高、重现性好、简便易行。     

广西产尖吻蝮蛇蛇毒细胞毒素的纯化与活性鉴定

采用SephadexG50,Source30Q及phenylsepharoseHP柱层析从广西五步蛇蛇毒分离纯化出一种电泳纯细胞毒素I3H3,分子质量为36ku。I3H3对体外培养的人癌细胞有较强抑制和杀伤作用。用溴化二苯四偶氮盐比色法检测其对体外培养的人癌细胞的细胞毒作用,探索量效关系。其对体外培养的人肺癌细胞株(A549),人胃癌细胞株(BGC)和人鼻咽癌细胞株(KB)的抑制作用呈明显剂量依赖关系,作用24h的IC50分别为27.75,26.90和64.46μg/m1。     

耳穴疗法治疗老人失眠症之临床研究

目的:尝试利用科学性之方法探讨耳穴磁疗对老人失眠症之效果.方法:120位60岁或以上之老年患者,随机分配入三组.第一组及第二组为对照组,分别贴有灯芯或王不留行籽(不加压);第三组为实验组,贴有每粒含66高斯之磁珠(不加压).为针对老年患者常见之脏腑功能衰弱而致失眠,各人均选取相同之穴位:神门、心、肾、肝、脾、皮质下及枕,疗程共3周.结果:疗程完毕后,使用磁珠作耳疗之实验组于治疗前后的睡眠效果指数有明显之改进(P＜0.05),显效17%,有效为65%,总有效率为82%.此外,利用多元回归之反向淘汱法分析女性参加者之数据,发现利用磁珠作耳疗及参加者年龄对预测睡眠效果指数达显著水准(F3,106=9.04,P＜0.001,R2=0.20).结论:耳穴磁疗能有效地改善老人失眠症.     

Inducible nitric oxide synthase expression elicited in the mouse brain by inflammatory mediators circulating in the cerebrospinal fluid

Expression of inducible nitric oxide synthase (iNOS) protein was studied in the brain after intracerebroventricular injections of interferon (IFN)-gamma, and IFN-gamma combined with lipopolysaccharide (LPS) or tumor necrosis factor (TNF)-alpha, compared to ovalbumin as control. Wild-type mice and mice with targeted deletion of the IFN-gamma receptor gene were used. Findings based on iNOS immunoreactivity were evaluated at 1, 2, 4 and 7 days post-injection, using also quantitative image analysis and double labeling with glial cell markers. IFN-gamma administration induced iNOS immmunostaining in activated microglia and macrophages in the parenchyma surrounding the ventricular system, several cortical fields and fiber tracts. IFN-gamma-elicited iNOS immunoreactivity was down-regulated after 1 day. The number of iNOS-immunopositive cells was significantly enhanced by co-administration of LPS or TNF-alpha; IFN-gamma+TNF-alpha injections also resulted in longer persistence of iNOS immunoreactivity. No immunopositive cells were seen in the brain of IFN-gamma receptor knockout mice after IFN-gamma administration; very few immunostained macrophages were detected in these cases, mostly around the injection needle track, after co-administration of LPS or TNF-alpha. Western blot analysis confirmed a marked iNOS induction in the brain of wild-type mice 24 h after IFN-gamma+LPS injections. The findings show that inflammatory mediators circulating in the cerebrospinal fluid induce in vivo iNOS in the brain with topographical selectivity and temporal regulation. The data also demonstrate that the signaling cascade activated by IFN-gamma binding to its receptor is critical for iNOS induction, and the synergistic action of LPS and TNF-alpha as iNOS inducers in brain cells is largely mediated by the receptor-regulated action of IFN-gamma.     

人工耳蜗植入术后植入电极的影像学检查

目的 探讨建立螺旋CT扫描及三维重建技术观察人工耳蜗植入电极方法，并比较X线摄片方法与螺旋CT扫描三维重建方法的耳蜗内电极的影像学特征及其临床应用价值。方法 18例人工耳蜗植入患者全部作术后X线摄片检查。其中9例用经眼眶前后位摄片，9例采用侧斜位60。摄片。3例患者施行术后螺旋CT扫描及内耳三维重建方法。结果 2种投射头位的X线摄片均可显示电极形态及单个电极对，可间接判断电极在耳蜗内的植入深度。螺旋CT扫描三维重建图可直观地显示耳蜗形态、电极形态及其在耳蜗内植入的深度，可清晰识别单个电极对。结论 螺旋CT扫描三维重建方法可直观观察植入电极的形态及位置，可准确判断电极在耳蜗内植入的深度，有其独特的临床应用价值。