Immunocytochemical and biochemical characterization of the connective tissue component of fibrous papular lesions of oral mucosa

In an attempt to define the fibroblastic components of fibrous papular lesions of oral mucosa. 21 cases representing these lesions were selected for study. Paraffin or frozen sections of the lesions were stained, using the immunoperoxidase technique, with antibodies to: alpha-l-antitrypsin. alpha-l-antichymotrypsin. lysozyme. fibro-nectin. act in. myosin, and for Clq binding Three of these cases were biochemically analyzed for collagen Types 1. III. and V. This study demonstrated the presence of cytochemical markers in fibrous papules that were similar to those observed for the compartment of circumvascular fibroblasts in control normal mucosa. Analysis of the collagens present indicated that in addition to histologic similarities, the gene products of these extracellular matrices were similar to those reported for angiofibromas and Shagreen patches in tuberous sclerosis. The cells in these lesions appear to be distinct from myofibroblasts and the high affinity complement binding fibroblasts characterized by Bordin et al. (1).     

MR imaging of the acutely injured patient with cervical traction

Magnetic resonance (MR) imaging of the patient with acute cervical injury is important because of the potential prognostic significance of the appearance of the spinal cord at the time of injury. However, cervical traction may involve equipment incompatible with the magnetic environment, and transferring the patient to the imaging table may make it difficult to maintain traction. The authors describe a simple, inexpensive, and reliable method for providing cervical traction within the magnet room.     

Cost-effectiveness of collaborative care for chronically ill patients with comorbid depressive disorder in the general hospital setting,a randomised controlled trial

Background  

Depressive disorder is one of the most common disorders, and is highly prevalent in chronically ill patients. The presence of comorbid depression has a negative influence on quality of life, health care costs, self-care, morbidity, and mortality. Early diagnosis and well-organized treatment of depression has a positive influence on these aspects. Earlier research in the USA has reported good results with regard to the treatment of depression with a collaborative care approach and an antidepressant algorithm. In the UK 'Problem Solving Treatment' has proved to be feasible. However, in the general hospital setting this approach has not yet been evaluated.     

Effects of epitopes combination and adjuvants on immune responses to anti-Alzheimer disease DNA vaccines in mice

Alzheimer disease （AD） is a neurodegenerative disorder characterized by neuropathological hallmarks including deposits of the beta-amyloid peptide （AssP）. Studies have shown that immunization with Abeta42 peptide reduces both the spatial memory impairments and Alzheimer disease-like neuropathologic changes in Alzheimer disease transgenic mice, but can cause side effect of a cell-mediated autoimmune meningoencephalitis. Recently, some studies showed that DNA vaccination could be used to generate an antibody response to Abeta without the adverse cell-mediated immune effect. In the current study, we generate four DNA vaccine plasmlds （pV-GE1, pV-GE2, pV-GE3, and pV-GE4） against Alzheimer disease by separately fusing Abeta epitope sequences （coding for EFGH, DAEFGH, EFGH ＋ EFGH, and EFGH ＋ DAEFGH） with IgG heavy chain coding region of mouse. Meanwhile, the full-length gene Abeta encoding plasmid （pV-Abeta）, empty vector （pVAX） and synthetic AssP were also included as control. The sera of BALB/c mice immunized via intramuscular with plasmids and peptide were tested by indirect ELISA for auto-AssP immunoreactivity. The results showed that all the DNA vaccine plasmids induced AssP-specific antibodies; moreover pV-GE2 and pV-Abeta constructs elicited higher antibody titers than other constructs （P〈0.05）. To further enhance the immune response, GM-CSF encoding plasmid （pGM-CSF） and purified BCG-DNA were used as molecular adjuvants. BCG-DNA could enhance humoral and cellular immune responses simultaneously and did not alter the phenotype of the immune responses,     

Improved Magnetic Resonance Molecular Imaging of Tumor Angiogenesis by Avidin-Induced Clearance of Nonbound Bimodal Liposomes

Angiogenic, that is, newly formed, blood vessels play an important role in tumor growth and metastasis and are a potential target for tumor treatment. In previous studies, the α_vβ₃ integrin, which is strongly expressed in angiogenic vessels, has been used as a target for Arg-Gly-Asp (RGD)-functionalized nanoparticulate contrast agents for magnetic resonance imaging-based visualization of angiogenesis. In the present study, the target-to-background ratio was increased by diminishing the nonspecific contrast enhancement originating from contrast material present in the blood pool. This was accomplished by the use of a so-called avidin chase, which allowed rapid clearance of non-bound paramagnetic RGD-biotin-liposomes from the blood circulation. C57BL/6 mice, bearing a B16F10 mouse melanoma, received RGD-functionalized or untargeted biotin-liposomes, which was followed by avidin infusion or no infusion. Precontrast, postcontrast, and postavidin T₁-weighted magnetic resonance images were acquired at 6.3 T. Postcontrast images showed similar percentages of contrast-enhanced pixels in the tumors of mice that received RGD-biotin-liposomes and biotin-liposomes. Post avidin infusion this percentage rapidly decreased to precontrast levels for biotin-liposomes, whereas a significant amount of contrast-enhanced pixels remained present for RGD-biotin-liposomes. These results showed that besides target-associated contrast agent, the circulating contrast agent contributed significantly to the contrast enhancement as well. Ex vivo fluorescence microscopy confirmed association of the RGD-biotin-liposomes to tumor endothelial cells both with and without avidin infusion, whereas biotin-liposomes were predominantly found within the vessel lumen. The clearance methodology presented in this study successfully enhanced the specificity of molecular magnetic resonance imaging and opens exciting possibilities for studying detection limits and targeting kinetics of site-directed contrast agents in vivo.     

血浆甘磷酰芥及代谢物的测定方法和大鼠口服药代动力学

利用甘磷酰芥与4-(4′-硝基苯甲基)-吡啶(NBP)呈显色反应建立了该药及其代谢物在血浆及尿中的定量测定方法。观察了血浆在体外对该药的代谢作用及种属差异。研究了大鼠口服该药的药代动力学。     

清热中药对大鼠下丘脑组织AVP含量的影响

目的：清热中药大多具有一定的解热作用,为探讨其解热作用机理,我们选用典型的清热中药黄芩,银花,连翘组成清热方,从体温调节中枢神经介质方面来研究其解热作用机制。方法;选用Ｗｉｓｔａｒ大鼠,按体重随机分作３组,背部皮下注射酵母混悬液制造发热模型,给药组灌服清热方颗粒剂,模型组予等量饮用水,正常对照组不作处理,给药２ｈ后断头取脑,用放免方法检测大鼠下丘脑组织中ＡＶＰ含量。结果：模型组大鼠体温和下丘脑组织     

反相高效液相色谱法测定甲磺酸酚妥拉明片的含量

目的：建立了反向高效液相色谱法测定甲磺酸酚妥拉明的含量的方法。方法：采用Symmetry C18硅烷键和硅胶柱,甲醇－水－冰醋酸－三乙胺（10：10：1：0．1）作为流动相,检测波长为278nm,峰面积外标法测定。结果：平均回收率为100．2％（n=11),日内、日间差分别为0．7％和0．8％,在10－250μg范围内线性关系良好（r=0.9999),结论：该法简便、准确、结果满意。