Characteristics of copper corrosion in simulated uterine fluid in the presence of protein

In order to acquire more knowledge of the performance of copper-bearing intrauterine devices (Cu-IUDs), corrosion behavior of copper in a simulated uterine fluid was investigated in the presence of proteins. The proteins studied included serum albumin, -globulin and hemoglobin. Electrochemical polarization resistance measurements indicated that, in all cases under study, the corrosion rate of copper declined with time and the proteins always increased the rate. Moreover, different kinds of protein showed different dependence of copper corrosion rate on protein level. Addition of serum albumin initially raised the copper corrosion rate; however, the higher albumin concentration resulted in less promotion of the corrosion. X-ray diffraction (XRD) of the specimen surface showed that, both in the presence and absence of serum albumin, cuprous oxide was the only corrosion product, even though at higher concentrations of albumin less cuprous oxide was formed. This implies that serum albumin does not alter the corrosion mechanism. Electrochemical cathodic reduction of the oxide film and chemical analysis of solution after corrosion testing proved that the proportion of soluble ionic copper in the whole corrosion products varied with the albumin level. The copper corrosion rate in the presence of -globulin or hemoglobin increased monotonically with increasing concentration of the protein. These three proteins shifted the corrosion potential of copper towards negative. It could be inferred that the proteins accelerated the anodic dissolution process of copper and hence enhanced its corrosion.     

c-fos基因在乐果中毒后肌无力大鼠肌组织中的表达

采用ＲＴ－ＰＣＲ、ＷｅｓｔｅｒｎＢｌｏｔ技术,检测了有机磷农药乐果对大鼠骨骼肌细胞中即刻早期反应基因ｃ－ｆｏｓ表达的影响。结果表明,在乐果作用下,骨骼肌细胞ｃ－ｆｏｓ基因表达在ｍＲＮＡ以及蛋白质水平均明显增高。然而骨骼肌细胞ｃ－ｆｏｓ的表达是否与毒物刺激有关,其在农药中毒机制上的生物学意义有待深入研究。     

老年哮喘发作期血浆内皮素含量的研究

目的 探讨血浆内皮素在老年哮喘发作期的作用。方法用放射免疫法测定健康老年人35例,非老年人30例和老年哮喘发作期37例血浆内皮素含量。结果老年哮喘发作期内皮素含量为(107、26±831)g／ml)明显高于恢复期(52、13±6.74pg／ml),两组差异性非常显(P<0.01);而恢复期虽然明显降低,但仍高于健康老年组(38．24±5．16pg／ml)与非老年组(31.16±4．64pg／ml)差异性也非常显(P<0．01);而健康老年与非老年组无差异性。结论老年哮喘发作期内皮素含量明显增高,说明内皮素与哮喘发作有关,认为内皮素升高可能是老年哮喘发作的一种内源性致病因素,测定其含量为老年哮喘发作的防治提供了新途径。     

针灸治疗三叉神经痛的文献述评

根据近10年所发表的针灸现代文献，对针灸治疗三叉神经痛的选穴特点、针灸治疗规律、疗效进行评价，并对目前临床研究中存在的问题及今后研究的重点提出了建设性的意见。     

益肾通脑宁治疗偏头痛的神经化学机理研究

目的：研究益肾通脑宁对大鼠脑内神经递质水平的影响。方法：皮下注射氢化可的松诱发大鼠肾阴虚，并同时灌服益肾通脑宁，测定大鼠下丘脑中DA和5-HT含量。结果：益肾通脑宁可明显增加氢化可的松肾阴虚模型大鼠下丘脑中5-HT的含量，时DA含量无明显影响。结论：益肾通脑宁可能是通过升高脑内5-HT水平而发挥治疗作用。     

卡铂栓剂溶出度实验方法研究

目的：建立测定卡铂栓溶出度的实验方法，提供药品评价和质控的依据。方法：溶出度的测定采用比色法，水平滚筒溶出装置，蒸馏水(pH5～7)为溶出介质。结果：卡铂栓剂溶出度5小时为65％以上，6小时为80％以上。结论：此方法准确、快速、简便。为控制卡铂栓的质量，有必要进行溶出度测定。     

半夏厚朴汤及其组成中药抗焦虑和抑郁研究进展

目的：综述半夏厚朴汤及其组成中药抗焦虑和抑郁研究进展。方法：查阅国内外近年来有关文献结合相关的研究成果，从半夏厚朴汤对动物焦虑和抑郁行为、神经生化机制、免疫系统功能、血脂代谢、机体氧化防御系统等层次进行阐述。结果与结论：半夏厚朴汤及其组成中药抗焦虑和抑郁具有一定的科学性。     

Clinical analysis of 276 cases of non-palpable TO breast cancer

Objective Discussion of diagnosis, treatment and prognosis of non-palpable TO breast cancer. Methods Between 1978 and 1997, 9,980 female patients with operable breast cancer were treated surgically, of which 276 were determined to have TO breast cancer. Most TO breast cancers could be detected promptly with careful examination of presenting symptoms, such as nipple discharge, local thickening of the breast, nipple erosion, nipple retraction and postmenopausal mastalgia, while 12 cases were detected by routine mammography of the contralateral breast. Results All patients were treated surgically and their tissue subjected to histopathological examination. Most cases (73.0%) were noninvasive or early invasive carcinoma. Axillary lymph nodes metastases were found in 7.69% of 234 mastectomy cases. Conclusion The survival rate was significantly increased if the tumor was in an early stage. The 5-, 10-, 15-years survival rates were 98.1%, 94.6% and 90.3%, respectively.     

肺癌中p53和p63 蛋白表达的高通量组织微阵研究

目的探讨p53和p63蛋白在肺癌中的表达及意义,并比较两者之间的表达关系.方法组织微阵(tissue microarray,TMA)技术和免疫组化S-P方法(immunohistochemistry,IHC).结果p53蛋白IHC染色中,TMA有价值的肿瘤标本为234(72.9%),总的表达率(+、++、+++)率为48.3%(113/234).随着肺鳞状细胞癌(squamous cell carcinoma,SCC)和腺癌(adenocarcinoma,Ad)分级升高,p53蛋白表达增强,与肺SCC及Ad病理分级有明显相关性(P＜0.05).p53蛋白表达与生存期呈明显负相关性(P＜0.05).p53蛋白表达与病理分型和临床分期等无明显相关性(P＞0.05).p53蛋白表达在肺癌原发肿瘤和转移淋巴结之间无明显差异(P＞0.05).p63蛋白IHC染色中,TMA有价值的肿瘤标本为264(82.2%),总的表达率(+、++、+++)为67.8%(179/264).p63蛋白在小细胞肺癌(small cell lung carcinoma,SCLC)表达最低16.0%(4/25),非小细胞肺癌(non-small cell lung carcinoma,NSCLC)与SCLC之间表达存在明显差异(P＜0.05).p63蛋白表达与病理组织分级、临床分期、生存期等无明显相关性(P＞0.05).p63蛋白表达在肺癌原发肿瘤和转移淋巴结之间无明显差异(P＞0.05).结论1.p53是肺癌的独立预后指标;2.p63蛋白在肺癌的不同病理类型的不同表达,说明肺癌的不同病理类型分子机理有所不同;3.p53蛋白和p63蛋白表达之间无明显相关性;4.TMA可以有效分析比较组织中肿瘤标记物的表达情况.