全文获取类型
收费全文 | 1228篇 |
免费 | 46篇 |
国内免费 | 81篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 67篇 |
妇产科学 | 9篇 |
基础医学 | 134篇 |
口腔科学 | 23篇 |
临床医学 | 221篇 |
内科学 | 293篇 |
皮肤病学 | 33篇 |
神经病学 | 27篇 |
特种医学 | 300篇 |
外科学 | 57篇 |
综合类 | 26篇 |
预防医学 | 28篇 |
眼科学 | 6篇 |
药学 | 71篇 |
肿瘤学 | 59篇 |
出版年
2022年 | 5篇 |
2021年 | 8篇 |
2019年 | 11篇 |
2018年 | 7篇 |
2017年 | 9篇 |
2016年 | 10篇 |
2015年 | 18篇 |
2014年 | 11篇 |
2013年 | 26篇 |
2012年 | 10篇 |
2011年 | 14篇 |
2010年 | 48篇 |
2009年 | 45篇 |
2008年 | 18篇 |
2007年 | 56篇 |
2006年 | 17篇 |
2005年 | 25篇 |
2004年 | 9篇 |
2003年 | 12篇 |
2002年 | 17篇 |
2001年 | 16篇 |
2000年 | 19篇 |
1999年 | 24篇 |
1998年 | 93篇 |
1997年 | 88篇 |
1996年 | 109篇 |
1995年 | 84篇 |
1994年 | 87篇 |
1993年 | 74篇 |
1992年 | 21篇 |
1991年 | 30篇 |
1990年 | 27篇 |
1989年 | 32篇 |
1988年 | 36篇 |
1987年 | 27篇 |
1986年 | 34篇 |
1985年 | 32篇 |
1984年 | 13篇 |
1983年 | 21篇 |
1982年 | 20篇 |
1981年 | 21篇 |
1980年 | 17篇 |
1979年 | 4篇 |
1978年 | 9篇 |
1977年 | 14篇 |
1976年 | 13篇 |
1975年 | 7篇 |
1970年 | 1篇 |
1969年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有1355条查询结果,搜索用时 15 毫秒
961.
962.
963.
964.
Berthou C; Marolleau JP; Lafaurie C; Soulie A; Dal Cortivo L; Bourge JF; Benbunan M; Sasportes M 《Blood》1995,86(9):3500-3506
Granzyme B and perforin are cytoplasmic granule-associated proteins used by cytotoxic T lymphocytes and natural killer (NK) cells to kill their targets. However, granzyme B gene expression has also been detected in a non-cytotoxic hematopoietic murine multipotent stem cell line, FDCP-Mix. The objective of the present study was to investigate whether granzyme B and perforin could be expressed in human hematopoietic CD34+ cells and if present, discover what their physiologic relevance could be. The primitive CD34+ human cell line KG1a was investigated first and was found to express granzyme B and perforin. Highly purified hematopoietic stem/progenitor cells were then selected using the CD34 surface antigen as marker. Steady-state bone marrow (BM) CD34+ cells did not contain these proteins. Peripheral blood (PB) CD34+ cells, which had been induced to circulate, were also analyzed. After chemotherapy (CT) and granulocyte colony-stimulating factor (G-CSF) treatment, CD34+ cells strongly expressed mRNAs and proteins of granzyme B and perforin. In contrast, CD34+ cells mobilized by G-CSF alone were negative. Western blot analysis further showed that granzyme B and perforin proteins were identical in CD34+ cells and activated PBLs. Such proteins might be implicated in the highly efficient migration of CD34+ stem/progenitor cells from BM to PB after CT and G-CSF treatment. The cellular adhesion mechanisms involved in the BM homing of CD34+ cells are disrupted at least temporarily after CT. The Asp-ase proteolytic activity of granzyme B on extracellular matrix proteins could be used by progenitor cells for their rapid detachment from BM stromal cells and perforin might facilitate their migration across the endothelial cell barrier. 相似文献
965.
966.
967.
968.
Soulier J; Grollet L; Oksenhendler E; Cacoub P; Cazals-Hatem D; Babinet P; d'Agay MF; Clauvel JP; Raphael M; Degos L; Sigaux F 《Blood》1995,86(4):1276-1280
Multicentric Castleman's disease (MCD) is an atypical lymphoproliferative disorder defined using clinical and pathologic criteria. A characteristic of the MCD is a close association with Kaposi's sarcoma (KS), which occurs during the clinical course of most human immunodeficiency virus (HIV)-associated MCD cases and also, but less frequently, in HIV-negative patients. Recently, sequences of a putative new Herpesvirus (KSHV) have been isolated and further detected in almost all the acquired immunodeficiency syndrome (AIDS) KS and in most of the non-AIDS KS samples. In this study, we searched for these Herpesvirus-like sequences in MCD samples of 31 patients. KSHV sequences were detected in 14 of 14 cases of HIV-associated MCD, including 5 cases without detectable KS. Moreover, KSHV was detected in 7 of 17 MCD cases in HIV-negative patients, including 1 case associated with a cutaneous KS. In 34 non-MCD reactive lymph nodes (follicular and/or interfollicular hyperplasia) in HIV-negative patients, KSHV was detected in only 1 case. In 1 HIV-negative case of MCD, KSHV was found in both the lymph node and peripheral blood samples. These data suggest that KSHV could play a role in the pathogenesis of MCD, especially in HIV-infected patients. 相似文献
969.
970.