全文获取类型
收费全文 | 2307篇 |
免费 | 86篇 |
国内免费 | 8篇 |
专业分类
耳鼻咽喉 | 7篇 |
儿科学 | 16篇 |
妇产科学 | 12篇 |
基础医学 | 345篇 |
口腔科学 | 65篇 |
临床医学 | 123篇 |
内科学 | 480篇 |
皮肤病学 | 36篇 |
神经病学 | 215篇 |
特种医学 | 51篇 |
外科学 | 403篇 |
综合类 | 7篇 |
预防医学 | 106篇 |
眼科学 | 6篇 |
药学 | 267篇 |
中国医学 | 4篇 |
肿瘤学 | 258篇 |
出版年
2022年 | 16篇 |
2021年 | 31篇 |
2020年 | 14篇 |
2019年 | 21篇 |
2018年 | 20篇 |
2017年 | 16篇 |
2016年 | 22篇 |
2015年 | 17篇 |
2014年 | 44篇 |
2013年 | 50篇 |
2012年 | 109篇 |
2011年 | 98篇 |
2010年 | 53篇 |
2009年 | 35篇 |
2008年 | 80篇 |
2007年 | 98篇 |
2006年 | 118篇 |
2005年 | 115篇 |
2004年 | 111篇 |
2003年 | 114篇 |
2002年 | 134篇 |
2001年 | 116篇 |
2000年 | 125篇 |
1999年 | 108篇 |
1998年 | 33篇 |
1997年 | 26篇 |
1996年 | 28篇 |
1995年 | 12篇 |
1994年 | 23篇 |
1993年 | 22篇 |
1992年 | 82篇 |
1991年 | 50篇 |
1990年 | 45篇 |
1989年 | 49篇 |
1988年 | 61篇 |
1987年 | 44篇 |
1986年 | 38篇 |
1985年 | 35篇 |
1984年 | 32篇 |
1983年 | 25篇 |
1982年 | 11篇 |
1981年 | 11篇 |
1980年 | 15篇 |
1979年 | 31篇 |
1978年 | 13篇 |
1977年 | 20篇 |
1976年 | 11篇 |
1975年 | 4篇 |
1965年 | 3篇 |
1924年 | 2篇 |
排序方式: 共有2401条查询结果,搜索用时 78 毫秒
11.
Youngnam Kang Takashi Okada Harunori Ohmori 《The European journal of neuroscience》1998,10(4):1363-1375
We report here on the ionic mechanisms underlying the depolarizing afterpotential (DAP) in neocortical pyramidal cells, with special interest in those underlying the burst afterdischarge. Injections of short depolarizing current pulses under whole-cell current clamp with a CsCl-based internal medium generated, in most pyramidal cells, a single action potential with a plateau phase (plateau-AP), followed by a slowly decaying DAP both in the absence and presence of TTX. Under voltage-clamp, the same cells displayed a slow tail current (tail-I) at the offset of depolarization. When intracellular free Ca2+ was chelated with 10 mm BAPTA or when extracellular Ca2+ was replaced with equimolar Ba2+, neither the slow DAP nor the slow tail-I was observed. Extracellular application of Co2+ or Cd2+ reduced Ca2+ currents and the slow tail-I. Cation substitution experiments revealed that the channel generating the slow tail-I was permeable to K+ and Cs+ more than to Na+ (PK≈PCs > PNa > PNMDG≈PTEA). The cationic slow tail-I was not reduced by applying antagonists of the metabotropic glutamate receptor (MCPG, 1 mm ) and the muscarinic receptor (atropine, 1–10 μm ). Thus, the slow DAP was produced by activation of the cationic channel whose gating is solely dependent on [Ca2+]i. An increase in [K+]o from 3 to 6 or 9 mm enhanced the slow DAP, and resulted in a generation of burst afterdischarges. An anticonvulsant, phenytoin (PT; 1–10 μm ) suppressed the slow DAP while enhancing the plateau-AP in the presence of TTX, most likely by blocking the cationic channel. 相似文献
12.
13.
Spondylolysis after posterior decompression of the lumbar spine: 35 patients followed for 3-9 years 总被引:5,自引:0,他引:5
Radiographs were examined in 35 patients who had had posterior decompression without fusion of the lumbar spine. Spondylolysis was found in 10 patients. Segmental range of motion, degree of vertebral slippage and width of decompression were analyzed by radiography. There was greater vertebral slippage after surgery in patients with postoperative spondylolysis than in those without spondylolysis. We conclude that excessive bony decompression may cause postoperative spondylolysis. 相似文献
14.
H Ohmori T Ohashi Y Aso Y Kumamoto H Hisazumi Y Shiraiwa T Kurita J Shimazaki T Ohkawa H Ogawa 《Hinyokika kiyo. Acta urologica Japonica》1988,34(12):2101-2110
We have investigated the clinical significance of urinary tissue polypeptide antigen (TPA) as a tumor marker for urothelial cancers. Urinary TPA levels were determined by the immunoradiometric assay of Prolifigen TPA Kit "Daiichi"-II in 486 healthy controls and 1835 patients with various diseases including 526 with urothelial cancers and 140 with prostatic cancer. The mean value of urinary TPA was 199 +/- 213 (1SD)U/1 in 486 healthy controls. 95% of them having a level below 600 U/l. Therefore, 600 U/l was applied as a cut-off level. Positive rates of urothelial cancers and reactivated prostatic cancer were 57.6% (148 of 248 cases) and 45.5% (5 of 11 cases) respectively. On the other hand, the false positive rate of most urological benign diseases was only about 20% except for the acute stage of urinary tract infections and upper urinary tract stones with hydronephrosis. There was no significant difference in the positive rate between urinary TPA level and urinary cytology in urothelial cancers. The combination of both tests raised the positive rate to 73.1%. Therefore, urinary TPA may be useful in the monitoring of urothelial cancers, and the combination of urinary TPA and urinary cytology may increase the diagnostic accuracy. 相似文献
15.
Takaaki Ohmori Ryo Tabei Norimasa Arita Mari Kondo Tetsuo Kozawa Yoshinori Chikamori Norifumi Ueda 《Pathology international》1983,33(5):999-1007
This report concerns a case of solitary extramedullary plasmacytoma of the left submandibular lymph node in a 56-year-old man. The tumor showed monoclonal proliferation of abnormal plasma cells which revealed highly positive stainings of both methylgreen pyronin and kappa light chain using the immunoperoxidase technique in the cytoplasms, and further revealed massive'.amyloid'deposits in the stroma, which suggested the possibility of sequential amyloid formation upon the secretion of paraprotein by tumor cells. 相似文献
16.
17.
Tomoko Morikawa Ichiro Yamadori Takeshi Okino Tetsuro Ogino Masaki Ohmori 《Pathology international》1992,42(4):242-248
Two cell lines designated IC KMS and D KMS were established from human adenovirus type 12 induced tumors of C3Hf/OK mouse. The cell lines retained the characteristics of the original tumor i.e., production of numerous C type and intracisternal A-type particles, integration of Adl2 El region DNA and amplification of the myc gene family. Chromosomal analysis revealed chromosome aberrations in both IC KMS and D KMS cells. The modal chromosome number of IC KMS cells was 54 and that of D-KMS cells was 48. Metacentric chromosomes and mini-chromosomes were found. Trisomy of chromosome 3, 7 and 12 was seen frequently in D KMS cells. Although DNA aneuploidy was revealed by flow cytometry, the DNA indices of these cells showed no relation to the copy number of integrated Adl2 DNA. These cells have been propagated by serial culture during the past 17 months. Production of endogenous virus particles is a unique characteristic of IC KMS and D KMS cells. These cell lines would be useful materials for examining the contribution of Adl2 carcinogenesis to activation of endogenous virus particles, and also the correlation between Adl2 carcinogenesis and cancer related genes. Acta Pathol Jpn 42: 242-248, 1992. 相似文献
18.
Shinkai T De Luca V Zai G Shaikh S Matsumoto C Arnold PD Hwang R King N Trakalo J Potapova N Wong G Hori H Wong AH Ohmori O Nakamura J Kennedy JL 《Psychiatric genetics》2004,14(3):177-180
OBJECTIVE: Oxidative stress such as free radical-mediated neuronal dysfunction may be involved in the pathophysiology of schizophrenia. The human glutathione peroxidase (GPX1) is a selenium-dependent enzyme, which plays an important role in the detoxification of free radicals. We therefore hypothesized that the GPX1 gene, which is located on chromosome 3p21.3, may be involved in the pathophysiology of schizophrenia. The aim of this study is to examine whether a potentially functional polymorphism, a proline (Pro) to leucine (Leu) substitution at codon 197 (Pro197Leu) of the human GPX1 gene, is associated with susceptibility to schizophrenia. METHODS: We genotyped the Pro197Leu polymorphism in a total of 113 nuclear families that had a proband with schizophrenia. Genetic association was tested using the transmission disequilibrium test (TDT), the sib transmission disequilibrium test (STDT), and the family-based association test (FBAT). RESULTS: The minor allele (Leu) frequency was calculated to be 0.282. We could not find significant transmission disequilibrium of the alleles for the Pro197Leu polymorphism in the GPX1 gene in association with the presence of schizophrenia in our family sample (TDT, chi2=0.03, degrees of freedom=1, P=0.86; combined TDT-STDT, Z'=-0.052, P=0.47; FBAT, Z=0.000, P=1.000). CONCLUSION: The results of this study suggest that the GPX1 polymorphism is unlikely to be associated with susceptibility to schizophrenia. 相似文献
19.
Yoshio Hiasa Noriyuki Ito Masato Ohshima Tomoyoshi Ohmori Asao Seki Toshikazu Tatsumi Hiromu Yokoi Chikayoshi Iwata Yoshio Murata Nobuyuki Ito † 《Pathology international》1978,28(4):637-644
Light and electron microscopic studies have been made on an anaplastic giant-cell tumor that developed in a woman 8 years after an operation on the thyroid for papillary carcinoma. Many giant cells were observed in the anaplastic tumor tissue, but no follicles. Numerous tightly-packed mitochondria and abundant ribosomes were present, but there were no desmosomes. The basement membrane was not distinct. 相似文献
20.
Immune regulation by brain cells in the central nervous system: microglia but not astrocytes present myelin basic protein to encephalitogenic T cells under in vivo-mimicking conditions. 总被引:2,自引:0,他引:2
下载免费PDF全文
![点击此处可从《Immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The antigen-presenting capability of various types of brain cell, such as primary mixed glial cells, astrocytes and microglia, was examined under conditions in which Ia antigen expression on the cultured cells mimicked that in the central nervous system (CNS) of rats with experimental autoimmune encephalomyelitis (EAE). In the CNS of rats with EAE, microglia but not astrocytes express Ia antigens. To produce such conditions, cultured brain cells were treated with various concentrations of interferon-gamma (IFN-gamma). It was revealed that in vivo-like conditions were produced when cultured brain cells were treated with less than 100 U/ml IFN-gamma. Under such conditions, microglia presented an antigen, myelin basic protein (MBP), to MBP-specific T-cell lines. Astrocytes, on the other hand, did not show antigen-presenting ability, but rather suppressed T-cell proliferation. Primary mixed glial cells, mainly comprising astrocytes and microglia, were also weak antigen-presenting cells (APC). These findings suggest that brain cells comprising various types of cell with regard to APC function do not up-regulate the proliferation of encephalitogenic T cells in vivo, although a particular type of brain cell, i.e. microglia, show antigen-presenting capability. 相似文献