Concepts of healthy diet as expressed by primary health care workers in the national capital of Brazil

Dietary changes in Western society highlight the need for individual and collective health providers to use their strategic positions to actively promote healthy eating habits. Using the research-action methodology in various clinics in the Federal District of Brazil, the present study aimed to identify what these professionals consider a healthy diet. The results indicate an apparent conceptual dichotomy: on the one hand, an idealized version of eating for good health based on the scientific literature; on the other, a concept derived from the ideal, but based on people's day-to-day reality. In their pursuit of the latter concept, people in social situations make connections between various pieces of information, and what emerges are the eating habits most closely associated with a particular lifestyle. However, both concepts tend to transcend the implicit biological character of the conceptual constructs and to find support in the sociocultural conditions that shape them and which in turn are shaped at the concrete level of reality.     

Effects of oral glucose administration on plasma growth hormone levels in women with polycystic ovary syndrome

Objective: To evaluate the sensitivity of GH secretion to the suppressive effect of oral glucose administration in women with polycystic ovary syndrome (PCOS).

Design: Comparison of the GH response to an oral glucose load in women with PCOS and in weight-matched normally menstruating women (controls).

Setting: Reproductive endocrinology unit.

Patient(s): Eighteen obese and 11 nonobese patients and 10 obese and 10 nonobese controls.

Intervention(s): After an overnight fast, each woman underwent a 75-g, 3-hour oral glucose tolerance test (OGTT).

Mean Outcome Measure(s): Growth hormone, glucose, and insulin responses to OGTT.

Result(s): No significant differences in the glycemic and insulinemic responses were found between the patients and the weight-matched controls. No decrease in plasma GH was observed in both obese and nonobese patients and in obese controls during the OGTT, whereas a significant GH decrease occurred in nonobese controls 60 and 120 minutes after glucose intake.

Conclusion(s): Oral glucose administration was unable to suppress GH levels in nonobese as well as in obese women with PCOS and in obese control women. These data suggest that both PCOS and obesity are associated with a reduced sensitivity of GH secretion to glucose suppression.     

Evaluation of marrow and blood haemopoietic progenitors in chronic lymphocytic leukaemia before and after chemotherapy

Abstract: We have evaluated the number and differentiation pattern of CD34+ cells, as well as the CFU–GM, BFU–E and CFU–GEMM progenitors from the blood (PB) and marrow (BM) of 53 chronic lymphocytic leukaemia (CLL) patients. Twenty-four patients were untreated and 29 were studied at 2 months from the last course of fludarabine or chlorambucil; 6 patients, studied after fludarabine therapy, were further evaluated after mobilization with cyclophosphamide and G–CSF. PB of untreated patients showed a median number of CD34+ cells, CFU–GM, BFU–E and CFU–GEMM/10⁵ seeded cells and per litre of PB similar to those of normal controls. No differences were also found in the number of clonogenic progenitors/10⁵ cells in patients studied before and after therapy, while significantly fewer BFU–E/l of PB were found after fludarabine. The number of circulating CD34+ cells/l of PB was significantly lower in patients treated with fludarabine or chlorambucil compared to untreated patients. BM growth was significantly reduced in untreated CLL patients compared to healthy donors. Treatment with fludarabine or chlorambucil restored BM progenitors at levels similar to those of normal controls; this effect did not occur for CFU–GM in patients treated with fludarabine. Three-colour fluorescence analysis demonstrated a differentiation pattern of CD34+ cells, with a greater expression of CD13 and CD33 after treatment with fludarabine compared to untreated patients and normal controls. In 4 patients previously treated with fludarabine who underwent a successful cyclophosphamide and G-CSF mobilization therapy, 4 × 10⁶ CD34+ cells/kg were collected. These 4 patients showed a notable increase of CD34+ cells and of clonogenic cells in the PB, but a marked decrease of BM progenitor cells. The 2 patients who failed CD34+ cell mobilization had a reduced CFU-GM growth both in the PB and in the BM. Taken together, these studies indicate that residual haemopoietic progenitors are present in untreated CLL patients and that stem cell mobilization and collection can be carried out following fludarabine treatment.     

On Somatotopic Representation Centers for Finger Movements in Human Primary Motor Cortex and Supplementary Motor Area

We usedfunctional magnetic resonance imaging to examine the representation pattern for repetitive voluntary finger movements in the primary motor cortex (M1) and the supplementary motor area (SMA) of humans. Healthy right-handed participants performed repetitive individuated flexion-extension movements of digits 1, 2, and 3 using the dominant hand. Contralateral functional labeling for the group indicated a largely overlapping activation pattern in M1 and SMA for the three digits. Consistent with recent findings, the geographic activation center in M1 for each finger differed, and we found some evidence of a homunculus organization pattern in M1 and SMA, but only for the central location of the representations. However, the statistical power for the homunculus pattern was weak, and the distance separating the digit geographical centers was typically less than 15% of the entire extent of digit representations in M1 or SMA. While separations for digit representations occurred, the entire data set provided more support for the concept of distributed, overlapping representations than for a classic homunculus organization for voluntary finger movements.     

Combined visual attention and finger movement effects on human brain representations

Sensory and motor systems interact in complex ways; visual attention modifies behavior, neural encoding, and brain activation; and dividing attention with simultaneous tasks may impede performance while producing specific brain activation patterns. We hypothesized that combining voluntary movement with visual attention would yield unique brain representations differing from those occurring for movement or visual attention alone. Hemodynamic signals in humans were obtained with functional magnetic resonance imaging (MRI) while participants performed one of four tasks that required only a repetitive finger movement, only attending to the color of a visual stimulus, simultaneous finger movement and visual attention, or no movement and no visual attention. The movement-alone task yielded brain activation in structures commonly engaged during voluntary movement, including the primary motor cortex, supplementary motor area, and cerebellum. Visual attention alone resulted in sparse cerebral cortical and substantial bilateral cerebellar activation. Simultaneous performance of visual attention and finger movements yielded widespread cerebral cortical, cerebellar, and other subcortical activation, in many of the same sites activated for the movement or attention tasks. However, the movement-related plus attention-related activation extended beyond the movement-alone or attention-alone activation sites, indicating a novel activation pattern related to the combined performance of attention and movement. Additionally, the conjoint effects of visual attention and movement upon brain activation were probably not simple gain effects, since we found activation-related interactions in the left superior parietal lobule, the right fusiform gyrus, and left insula, indicating a potent combinatory role for visual attention and movement for activation patterns in the human brain. In conclusion, performing visual attention and movement tasks simultaneously, even though the tasks had no specific interrelationship, resulted in novel activation patterns not predicted by performing movements or visual attention alone. Electronic Publication