Electrophysiological study on differentiation of rat bone marrow stromal stem cells into neuron-like cells in vitro by edaravone

To explore the electrophysiological proper-ties of differentiation of rat bone marrow-derived stromal stem cells (rBMSCs) to neuron-like cells in vitro by edaravone, a new type of free radical scavenger. Methods: Stromal stem cells were separated from rat bone marrow with Ficoll-Paque reagent and expanded in different culture medium in vitro, rBMSCs were induced by edaravone containing serum-free L-DMEM. Morphologic observation and Western blot analysis including the ex-pression of Nav1.6, Kv1.2, Kv1.3, Cav1.2 were performed, and whole patch-clamp technique was used. Results: Cyton contraction and long processes were shown in differentiated stromal stem cells. Nav1.6, Kv1.2, Kv1.3 and Cav1.2 were expressed in both differentiated and undifferentiated cells. However, the expression of channel proteins in differentiated cells was up-regulated. Consistently, their resting potential and outward currents were also enhanced in the differentiated cells, which was especially significant in the outward rectifier potassium current. Conclusion: In vitro, neuron-like cells derived from rBMSCs, induced by edaravone, possess electrophysiologi-cal properties of neurons.     

Electrochemical investigation of formic acid electro-oxidation and its crossover through a Nafion® membrane

This work studies formic acid as an anode fuel for polymer electrolyte fuel cells and investigates the electro-oxidation of formic acid on Pt/C. Fuel crossovers through the Nafion^® 115 membrane at different temperatures and concentrations are reported. A linear dependence of the crossover current on the temperature and concentration is obtained. It is found that the crossover can be reduced by five times and a higher performance can be rendered by formic acid when compared to methanol under the same conditions. Electrochemical impedance measurements are conducted to interpret the reaction mechanism of formic acid oxidation. The effect of the electrode potential on the impedance pattern is revealed and an impedance model incorporating the reaction kinetics information is developed to simulate the experimental impedance response.     

对慢性心力衰竭病人治疗与护理的研究进展

心力衰竭已日益成为心血管病中对病人危害最大的疾病,因此对心力衰竭病人的治疗与护理应引起重视,现对心力衰竭病人的治疗与护理的研究进展进行了综述.     

Regulation of interleukin 6 expression in murine bone marrow stromal cells.

The regulation of interleukin 6 (IL-6) expression in the B-lymphocyte-supporting murine stromal cell line BMS2 has been examined in response to exogenous cytokines and chemical agents. Kinetic analyses of IL-6 mRNA induction and decay are presented together with analysis of the IL-6 biological activity. The cytokines tumor necrosis factor, interleukin 1 (alpha and beta), and transforming growth factor beta, as well as forskolin and dibutyryl cyclic AMP, all induce a transient rise in the steady-state level of IL-6 mRNA and an increased release of IL-6 protein. To study its regulation at the chromatin level, the murine IL-6 genomic gene has been cloned. Induction of IL-6 expression correlates with increased DNA nicking, consistent with increased topoisomerase I and endogenous nuclease activity. This finding is supported by kinetic analyses using camptothecin, a topoisomerase I inhibitor. We conclude that IL-6 regulation in murine stromal cells capable of supporting B-lymphopoiesis is comparable to that observed in human diploid fibroblasts.     

老年病人上腹部手术后应用PCEA的临床效果观察

目的　观察老年人上腹部术后病人自控硬膜外镇痛 (PCEA)的疗效及对内分泌功能的影响。方法　6 3例择期行胃癌根治术的老年患者 ,ASAⅠ -Ⅱ级 ,随机分为 3组 (每组 2 1例 ) :A组术后行硬膜外镇痛 (PCEA) ;B组术后行静脉镇痛 (PCIA) ;C组术后按需肌肉注射哌替啶镇痛 ;对比术后镇痛效果并分别于麻醉前 ,切皮后 90分钟 ,术后 6 0及 1、2、3天分别抽取外周静脉血测定血糖、胰岛素、皮质醇、肾上腺素浓度。结果　A、B组镇痛效果均满意 ,C组镇痛效果欠佳 ,综合镇痛质量A组优于B组优于C组 ;3组于切皮后 90分钟血皮质醇、血糖浓度均升高 ,但A组低于C、B两组 (P <0 .0 5 ) ,术后 6 0分钟均达高峰 (P <0 0 1) ,术后 2 4至 4 8小时虽有下降 ,但仍明显高于麻醉前水平 (P <0 0 5 ) ;血胰岛素水平 ,A、B组术后 3天无明显变化 ,C组术后第 1、2天升高显著 (P <0 .0 1及P <0 .0 5 ) ;肾上腺素A、B组术后无明显升高 ,C组术后 6 0分钟明显升高 ,于术后 3天仍未恢复至术前水平。结论　老年病人上腹部术后PCIA及PCEA均能达到优良的镇痛效果 ,PCEA能有效地抑制术后机体的应激反应 ,有利于术后病人的恢复。     

三种泻心汤及其类方不同配伍对正常大鼠胃粘液成分的影响

目的:探讨半夏泻心汤及其类方不同配伍对正常大鼠粘液含量的影响.方法:用均匀设计法对半夏泻心汤及其类方进行组方药量变化,以胃粘液含量为药理学指标,对所得的药理实验数据进行逐步回归分析.结果:与胃粘液含量关联度最大的为黄连,可以显著降低其含量,其次对半夏、大枣和甘草的交互项,可增加胃粘液含量.药物之间存在复杂的交互作用.结论:均匀设计为研究类方配伍提供了一种可供参考的试验设计方法.     

Oral manifestation of chronic mucocutaneous candidiasis: seven case reports

BACKGROUND: Chronic mucocutaneous candidiasis (CMC) is a rare disorder characterized by persistent or recurrent candidal infections of the skin, nails and mucous membranes or by a variable combination of endocrine failure as well as immunodeficiency. Oral clinicopathological features of CMC have seldom been described in detail. METHODS: Seven patients with CMC were reported in the study. The clinical and histological findings, etiological Candida species, immunological evaluation, and therapeutic pattern of oral lesions, were analyzed. RESULTS: Long-standing whitish hyperplastic and nodule-like lesions with exaggerated deep fissure were the typical and characteristic oral manifestations presented by all patients. The tongue was the most common site affected. Histologically, no obvious distinction was found between CMC and other forms of candidal infection. Abnormal proportions of T-lymphocyte subsets and positive titers of autoantibody were observed in three subjects (42.9%) and one patient (14.3%) respectively. Meanwhile, four subjects (57.1%) showed decreased albumin and increased globulin, three cases (42.9%) had high levels of ESR. But no iron deficiency was found. Candida albicans was the microorganism isolated from these patients. CONCLUSIONS: Multiple and widespread candidal infectious lesions can be observed on the oral cavity of CMC patients. Hyperplastic and nodule-like lesion with irremovable whitish patches and deep fissure are the most common oral manifestations of these patients. Dentists, otolaryngologists and pediatricians should be familiar with the clinical appearances of CMC to make an accurate diagnosis. Potential systemic disorders should be concerned to avoid the reoccurrence of oral candidiasis.     

停跳或不停跳心脏手术对血清 S-100B蛋白表达的影响

【目的】研究心脏手术围术期血清S-100B蛋白表达及其与停跳或不停跳心肺转流方式和时间的关系。【方法】体外循环心脏手术患者23例,测转流前、转流10min、转流末、转流后24h的血清S-100B蛋白表达水平。【结果】①血清S-100B蛋白质量浓度在体外循环前后动态变化:转流前(M)为0.27μg/L,转流10min后升至0.57μg/L(P<0.01),转流末达峰值1.80μg/L(P<0.01),转流后24h降为0.22μg/L(P>0.05)。转流末的血清S-100B蛋白质量浓度与转流时间呈正相关(r=0.488,P<0.05)。②停跳组(n=6)转流前、转流10min、转流末、转流后24h平均血清S-100B蛋白质量浓度分别为(0.17±0.09)μg/L、(0.48±0.13)μg/L、(1.65±0.52)μg/L和(0.19±0.04)μg/L,不停跳组(n=6)分别为(0.26±0.14)μg/L、(0.71±0.41)μg/L、(1.59±0.84)μg/L和(0.23±0.11)μg/L,两组差别无统计学意义(P>0.05)。【结论】体外循环可导致血清S-100B蛋白表达增高,其表达水平与心肺转流时间呈正相关,但与停跳或不停跳转流方式无关。