采用蛋白指纹图谱技术筛选大肠癌肝转移特异性相关蛋白

目的:应用表面增强激光解析电离飞行时间质谱技术从大肠癌及大肠癌肝转移患者中筛选出大肠癌肝转移患者血清特异性相关蛋白。方法:实验于2005-07/2006-09分别在南方医院消化中心实验室与解放军第一五○医院实验室完成。应用美国CipherGen公司IMAC3(ImmobilizedMetalAffinityCapture,金属亲和表面)芯片和蛋白芯片仪检测44例大肠癌患者及36例大肠癌肝转移患者血清中的蛋白质相对含量。设定所有血清样本检测的蛋白质相对分子质量区间在1500～20000。利用PBSⅡ型蛋白质芯片阅读仪对IMAC3芯片进行检测,所得到的蛋白质以波谱的形式表示。采用BiomarkerWizard软件对2组血清在相同质荷比的蛋白质含量数据进行方差分析,将分析所得到的含量有显著性差异(P<0.05)的蛋白质建立数据库,导入BiomarkerPattern智能统计分析软件,选择相应条件,对其进行分组统计,从而得到能够正确分组的特异性蛋白标志物并构建大肠癌肝转移的诊断模型。采用酶联免疫法检测相同血清标本中的CEA水平,与构建的诊断模型在大肠癌肝转移诊断中作比较。结果:①44例大肠癌患者与36例大肠癌肝转移患者的血清蛋白质在质荷比为2685.64～11813间有16个蛋白质含量有显著差异。②大肠癌组在质荷比为5909处的蛋白质的相对含量高于大肠癌肝转移组[(30.1±9.6)%,(14.5±10.4)%,P≤0.01]。③其中44例大肠癌患者中有38例患者被正确分组,36例大肠癌肝转移患者被正确识别,准确率为92.5%(74/80),灵敏度和特异性分别为100%(36/36),86.4%(38/44)。结论:表面增强激光解析电离飞行时间质谱技术快速、准确、灵敏度、特异性高,通过蛋白芯片仪发现的特异性相关蛋白,有望成为大肠癌肝转移诊断中有应用价值的临床检测指标。     

Human fetal bone marrow early progenitors for T, B, and myeloid cells are found exclusively in the population expressing high levels of CD34

Experimentation on human stem cells is hampered by the relative paucity of this population and by the lack of assays identifying multilineage differentiation, particularly along the lymphoid lineages. In our current study, phenotypic analysis of low-density fetal bone marrow cells showed two distinct populations of CD34+ cells: those expressing a high density of CD34 antigen on their surface (CD34hi) and those expressing an intermediate level of CD34 antigen (CD34lo). Multiple tissues were used to characterize the in vitro and in vivo potential of these subsets and showed that only CD34hi cells support long-term B lymphopoiesis and myelopoiesis in vitro and mediate T, B, and myeloid repopulation of human tissues implanted into SCID mice. CD34lo cells repeatedly failed to provide long-term hematopoietic activity in vivo or in vitro. These results indicate that a simple fractionation based on well-defined CD34 antigen levels can be used to reproducibly isolate cells highly enriched for in vivo long-term repopulating activity and for multipotent progenitors, including T- and B-cell precursors. Additionally, given the limited variability in the results and the high correlation between in vitro and in vivo hematopoietic potential, we propose that the CD34hi population contains virtually all of the stem cell activity in fetal bone marrow and therefore is the population of choice for future studies in hematopoietic stem cell development and gene therapy.     

身高是影响血管功能的主要因素

目的:探讨性别及其它生理因素对大动脉血管功能的影响.我们使用非创伤性血压压力波分析方法研究和探讨性别和身高对年长者中心和外周血流动力学变化的影响.方法:研究对年龄、身高、体重指数、心率和血压等等生理因素与系统动脉顺应性(SAC)和动脉压力增加指数(AI)等血流动力学参数之间的相关性和影响进行评估,共选择280个正常志愿者,其中男性98例,女性182例,年龄49～75岁.结果:结果经年龄校正后,脉搏波速度、系统动脉顺应性和动脉压力增加指数有显著的性别差异.系统动脉顺应性,动脉压力增加指数与高度相关,而且此相关在性别上有明显差异.经年龄和性别校正后身体体重指数与SAC正相关与AI负相关.经年龄、性别和身高校正后,脉搏波速度、中心动脉压增加指数与心率、中心脉压差呈显著地直线相关.结论:研究表明在正常老年人群中,性别影响动脉功能指数的测定,身高是影响系统动脉顺应性和动脉脉压增加指数的关键因素.     

Effects of music therapy on depression compared with psychotherapy

This paper reports a study testing the effects of music on depression and compares them with the effects of psychotherapy. There are mainly three conventional treatments for depression: psychotherapy, pharmaceutical treatments, and electroconvulsive therapy. Because conventional treatment has proven to be poorly successful, new means of treatment must be found that might improve depression when used together with other therapies. A randomized controlled clinical trial was performed with a convenience sample of 79 patients aged 25–60 years with low- and medium-grade depression. The Zung Depression Scale was employed for selection purposes. Patients were randomly assigned to the music-therapy group (classical and baroque music) (n = 41), or the psychotherapy group based on conductive-behavioral therapy (n = 38). The music therapy was applied for 50 min a day, every day, for eight weeks. At the end, the music-therapy group had less depressive symptoms than the psychotherapy group, and this was proven to be statistically significant with the Friedman test. We propose that patients with low- and medium-grade depression can use music to enhance the effects of psychological support.     

全髋关节置换术中钴-铬股骨头不同磨损部位的摩擦学特性

 通过使用原子力显微镜检查(atomic force microscopy,AFM)计算表面粗糙度和摩擦系数的方法,对工程和生物材料的摩擦学特性进行研究。尽管之前的大量研究对全髋关节置换术中不同承重材料的摩擦系数进行了报告,但却没有关于全髋关节置换术术中轴承材料的表面粗糙度和摩擦系数的关系的报道。此外,钴-铬股骨头不同磨损部位的摩擦学特性尚不明确。因此,本研究对全髋关节置换术后 10年钴-铬股骨头发生严重磨损及轻微磨损部位的表面粗糙度、摩擦系数和硬度之间的关系进行研究。钴-铬股骨头的平均维氏硬度为(380.7±11.3) HV。在 AFM扫描的 25滋m伊25滋m面积内,严重磨损和轻微磨损部位的摩擦系数分别为 0.229±0.054和 0.243±0.059,两者差异无统计学意义(P=0.449)。严重磨损部位的表面粗糙度[Rq=(96.5±26.2) nm]与轻微磨损部位[Rq=(17.7±4.2) nm]的差异有统计学意义(P <0.0001)。本研究结果显示院钴-铬股骨头的摩擦特性与其表面粗糙度没有明显关系,为金属植入材料表面特性的改善提供了依据。