双歧杆菌定量分析技术研究进展

传统细菌培养计数法是依据细菌表型特征来对双歧杆菌进行鉴别计数的,MTPY和BSM培养基是经常使用的双歧杆菌培养基．近年来包括点杂交、荧光原位杂交、荧光原位杂交结合流式细胞计数和荧光实时定量PCR技术在内的以核糖体及其编码基因核酸序列为鉴别基础的分子生物学技术被广泛用于双歧杆菌的定量定性分析,其中荧光原位杂交结合流式细胞计数和荧光实时定量PCR技术是目前较为理想的方法．     

超声促进4,4-二取代-2-苯基-2-噁唑啉-5-酮的水解

目的碱性水解4,4-二取代-2-苯基-2-     

有肠功能障碍的脊髓损伤病人直肠肛管生理检查

脊髓损伤病人常伴有严重的肠功能障碍 ,影响病人的社交活动和生活质量。本文目的是研究脊髓损伤病人的肠功能生理检查是否异常 ,这有助于理解脊髓损伤患者肠功能障碍的机理。　　方法 :对 12例有便秘、粪便嵌塞和肛门失禁症状的严重脊髓损伤病人进行肛管灌注测压、阴部神经终端运动潜伏期的测定和直肠内超声检查 ,其中女 6例 ,在脊髓损伤前均无任何肠道症状和直肠肛管手术史。中位年龄 5 4岁 (4 087岁 ) ,脊髓外伤 8例 (6 7% ) ,其中公路车祸 4例、跌落 3例、脊髓手术后 1例。其它损伤原因包括脊柱裂、脊髓空洞症、蛛网膜囊肿和腹主动脉瘤修…     

静脉注射乙酰半胱氨酸治疗慢性重型病毒性肝炎的临床观察

目的：观察乙酰半胱氨酸注射液（ＮＡＣ）治疗慢性重型肝炎的疗效。方法：收治慢性重型肝炎８８例,随机分为治疗组与对照组。对照组４４例采用前列腺素Ｅ１（ＰＧＥ１）,０．１－０．２ｍｇ／ｄ加入５％葡萄糖注射液中静脉滴注。治疗组４４例用３％ＮＡＣ２５０ｍｌ静脉滴注,每日一次,疗程一般均为一个月。结果：治疗组显效为６３．６％,有效为１１．３％,对照组显效为４５．４％,有效２２．７％,两组显效率比较有显著性差异     

Cytokines in therapy of radiation injury

Repeated injections or infusion of hematopoietic growth factors, such as interleukin-3 (IL-3), granulocyte macrophage-colony stimulating factor (GM-CSF), or granulocyte-colony stimulating factor (G-CSF), accelerate restoration of hematopoiesis in animals compromised by sublethal doses of cytotoxic drugs or irradiation. Previous work by the investigators has shown that IL-1 induced circulating CSF in normal mice and, when used after sublethal irradiation, accelerated the recovery of endogenous splenic colonies. Therefore, IL-1, as well as IFN-gamma, tumor necrosis factor (TNF), G-CSF, and GM-CSF, were evaluated as potential therapeutic agents in irradiated C3H-HeN mice. A single intraperitoneal injection, administered within three hours after a lethal dose (LD)95/30 of irradiation that would kill 95% of mice within 30 days, protected in a dose-dependent manner up to 100% of mice from radiation-induced death due to hematopoietic syndrome. Significant therapeutic effects were also achieved with a single dose of IFN-gamma or of TNF. In contrast, GM-CSF and G-CSF, administered shortly after irradiation, had no effect in the doses used on mice survival.     

Neonatal flumazenil therapy reverses maternal diazepam

A woman at 32 weeks' gestation with eclampsia was given 120 mg diazepam shortly before emergency caesarean section. The infant had persistent apnoea and required respiratory support. Spontaneous respiration began after intravenous flumazenil infusion was started. Diazepam and its active metabolites were assayed during and after 5 d of treatment with flumazenil.