Use of negative intrathoracic pressure to obtain end-systolic pressure volume relations in dogs

Left ventricular contractility can be assessed from the end-systolic pressure-volume relationship (ESPVR). In this study we test the hypothesis that the same ESPVR can be obtained by varying LV loading with different levels of negative intrathoracic pressure as by varying LV filling. In six dogs mean aortic transmural pressure was used to approximate LV end-systolic pressure and LV volume was determined from data gathered from biplane cineradiograms of multiple markers placed in the LV midwall. In each preparation right heart bypass allowed control of cardiac output while the thoracic pressure was varied with a box surrounding a midsternal thoracotomy. Reflex effects were minimized by ganglionic blockade and bilateral vagotomy. ESPVRs were obtained by varying the cardiac output at constant thoracic pressure or by changing intrathoracic pressure at constant cardiac output. The slopes of the ESPVRs were not significantly different. This result implies that LV loading by negative intrathoracic pressure, in this highly controlled preparation, can be used to generate a systolic LV elastance similar to that obtained by varying LV filling.     

彩色多普勒超声对肠套叠的诊断价值与治疗体会

肠套叠是外科常见急腹症之一，临床以小儿为多见，若不能及时诊治，可并发肠坏死和中毒性休克，甚至危及生命。我科自1998年至2007年12月，采用彩色多普勒超声对124例（共132人次）进行诊治，收到满意疗效，现将其诊断价值和治疗体会报告如下。     

CD54和LFA-1的相互作用在6A8α-甘露糖苷酶表达抑制的JurkatT细胞的黏附性增强中的作用

目的研究CD54和LFA-1的相互作用在6A8 α-甘露糖苷酶表达抑制的Jurkat细胞(AS)的黏附性增强中的作用。方法用细胞凝集试验确证AS细胞间黏附的增强，用细胞与细胞间黏附分子-1-人IgG的Fc片段(ICAM-1-Fc)的黏附试验和阻断性抗CD11a抗体的阻断试验研究CD54-LFA-1的作用，用单克隆抗体MEM-148检测As细胞LFA-1亲和力的变化，用单克隆抗体NKI-L16检测AS细胞LFA-1亲合力的变化，用鬼笔环肽染色细胞骨架，用Jurkat-Raji细胞间的作用作模型研究6A8α-甘露糖苷酶表达抑制对T和B细胞间黏附的影响，用ConA结合试验检测细胞中蛋白质N-糖基化的变化。结果(1)AS细胞间的黏附性增强主要与CD54及CD11a表达的增强相关，也与LFA-1亲和力的增高相关；(2)AS细胞的细胞骨架发生重排；(3)As细胞与Raji细胞间的黏附也增强；(4)ConA与AS细胞的结合增强。结论CD54和LFA-1的相互作用在AS Jurkat T细胞的黏附性增强中起重要作用。细胞骨架重排也可能起作用。As细胞的蛋白质发生了N-糖基化修饰。     

Diagnosis and taxonomy of periodical psychosis

The concept and taxonomy of periodic psychosis still remains in controversy for the past 100 years. In China, it was first reported by Ji, and thereafter various rinds of terminology and views were given by different authors. In the presentation, clinical investigation and follow up were carried out in 62 cases. In many of these cases, the so-called "periodic psychosis" or "phasophrenia" was early clinical features of schizophrenia or manic-depression. In those cases with apparent symptoms and signs of diencephalic function, differential diagnosis should be carried out from organic brain syndrome. In a small number of cases, their clinical manifestations, conforming to previous diagnostic criteria, might be an uncommon syndrome. It was concluded by the authors that until now periodic could be considered only as a clinical syndrome rather than a disease entity.     

Current Progress on Antibiotic Sensing Based on Ratiometric Fluorescent Sensors

Antibiotics, which can be used as veterinary drugs, are widely used in the prevention and treatment of infectious diseases for animals. However, overuse of antibiotics had caused serious problems on food contamination and human harm. For control such public issues, several of techniques have been in recent years. Ratiometric fluorescent (RF) technique, as one of the most promising strategies for quantitatively evaluated analytes, had been extensively developed for the readily measurements on the two different fluorescent emission intensities. In this review, the construction strategies for recent RF sensors will be mainly focused on. Meanwhile, the recent advances and new tendencies for detection of antibiotics based on RF technique shall be introduced. Finally, outlooks on the opportunities and challenges for quantitative fluorescence sensing on antibiotics will be summarized.

     

藜草花粉过敏与支气管哮喘的研究

为了确定藜草花粉过敏原对哮喘患者的致敏作用。对外源性哮喘患者用藜草花粉过敏原进行了皮肤挑刺试验（SPT）和支气管激发试验（BPT）。结果藜草花粉SPT阳性者为28.2％,18例BPT阳性者中,14例出现哮鸣音。藜草花粉BPT与SPT的符合率为85％。提示藜草花粉过敏原是我国哮喘患者的一个重要吸入性过敏原。