多次输注供体脾细胞诱导心脏移植免疫耐受的实验研究

目的　探讨供体脾细胞诱导心脏移植免疫耐受的作用。方法　将 5 0只行腹部心脏移植的纯系雄性Lewis大鼠 ,随机分为未处理组、一次脾细胞组、环磷酰胺组、一次脾细胞 环磷酰胺组、多次脾细胞 环磷酰胺组 ,每组 10只大鼠 ,以 5 0只纯系雄性DA大鼠为供体。观察移植心脏平均存活时间 (MST) ,移植后第 6天观察供体心脏病理学改变 ,供受体间的混合淋巴细胞反应 (MLR)、外源性白细胞介素 2 (IL 2 )对MLR的影响及体外过继转移实验。结果　多次脾细胞 环磷酰胺组供体心脏MST为 (85 3± 7 5 )d ,较未处理组 (7 3± 1 0 )d、一次脾细胞组 (7 9± 0 9)d、环磷酰胺组(8 1± 1 2 )d、一次脾细胞 环磷酰胺组 (2 5 8± 3 5 )d显著延长 (t=0 ,P <0 0 1) ;供体心脏仅见少量炎性细胞浸润 ;供受体间MLR较DA Lewis对照组显著降低 ,差异有显著意义 (P <0 0 1) ;外源性IL 2可以部分逆转DA Lewis耐受组MLR的低反应性 ;其免疫耐受状态可过继转移给正常的同系大鼠。结论　多次输注供体脾细胞联合应用环磷酰胺 ,可成功诱导同种大鼠心脏移植的免疫耐受。     

From gene networks to brain networks

The brain's structural organization is so complex that 2,500 years of analysis leaves pervasive uncertainty about (i) the identity of its basic parts (regions with their neuronal cell types and pathways interconnecting them), (ii) nomenclature, (iii) systematic classification of the parts with respect to topographic relationships and functional systems and (iv) the reliability of the connectional data itself. Here we present a prototype knowledge management system (http://brancusi.usc.edu/bkms/) for analyzing the architecture of brain networks in a systematic, interactive and extendable way. It supports alternative interpretations and models, is based on fully referenced and annotated data and can interact with genomic and functional knowledge management systems through web services protocols.     

Mechanical hypersensitivity and alterations in cutaneous nerve fibers in a mouse model of skin cancer pain

Melanoma inoculation induced marked mechanical allodynia and hyperalgesia in the periphery of the melanoma mass in mice from about day 10 post-inoculation. In the middle of the tumor, there were slight hyperalgesia and response disappearance in the early and late phases, respectively. PGP9.5-like immunoreactivities increased in the epidermis of the periphery of the tumor and disappeared from the dermis of the middle on day 18 post-inoculation, without apparent alterations on day 10. When using this pain model, one should consider the tumor site-dependent responses.     

美西律对QT延长综合症模型的作用(英文)

AIM：To make a LQT3 model(one form of the long QT syndromes)and to investigate the effect of mexiletine on LQT3.METHODS:Sea anemone toxin(ATX Ⅱ)was used to produce the LQT3 model.The Effect of mexiletine on LQT3 was performed on single Na channel,action potential,and electrocardiography in guinea pigs,RESULTS.With the binding of ATXⅡto the Na^ channels,the probability of being in the open state and the open time constant of single Na^ channel with long opening mode increased significantly.Action potential duration APD50,APD90,and the maximal upstroke velocity of phase 0 were increased by 25.8%,26.1%,and 12%,respectively.The QT interval and QTc,a rectifed QT interval,increased by 12.8% and 16.9%.On the contrary,after application of mexiletine,the open probability of single Na^ channel was reduced greatly.In the presence of ATXⅡ(40nmol/L),mexiletine(1,5,15,45,70μmol/L)shortened the APD50 by 0.5%,6.7%,14.4%,19.45,and 18.8%,respectively,and decreased the APD90and Vmax accordingly.In the experiments with ECG,mexiletine reversed the ATX0Ⅱ-produced prolongation effects on QTc in a dose-dependent manner.CONCLUSION:Mexiletine may be an effective drug in the treatment of LQT3.     

Relationship between the long type PML-RARalpha and the prognosis of patients with acute promyelocytic leukemia]

OBJECTIVE: To explore the relationship between long (L) type PML-RARalpha fusion gene and the prognosis of patients with acute promyelocytic leukemia (APL). METHODS: PML-RARalpha fusion gene was detected by RT-PCR in 33 APL patients. The optical density of three bands including E5 (+) E6 (+) 636 bp (no deletion), E5 (-) E6 (+) 492 bp (exon 5 deleted) and E5 (-) E6 (-) 232 bp (both exon 5 and exon 6 deleted) was measured by a UVP analysis system and their relative proportions were calculated. The relative expression level of each splicing band, initial WBC count and age were statistically (for single and multi-factor analysis) analyzed with prognosis. RESULTS: The relative expressions of E5 (-) E6 (+) and E5 (-) E6 (-) in death group were obviously different from that in first complete remission (CR1) group (P < 0.01), but do not for E5 (+) E6 (+) (P > 0.05). The relative expression levels of E5 (-) E6 (+), E5 (-) E6 (-) and E5 (+) E6 (+) were 0.23 +/- 0.12, 0.58 +/- 0.18, 0.20 +/- 0.09 in death group, and 0.45 +/- 0.16, 0.23 +/- 0.12, 0.31 +/- 0.16 in CR1 group, respectively. Initial WBC count and age was no difference between the two groups (P > 0.05). Logistic regression analysis showed that the expression of E5 (-) E6 (+) had no effect on the prognosis (B = 3.475, P = 0.492), but the expression of E5 (-) E6 (-) showed a negative correlation with prognosis (B = -19.660, P = 0.046). CONCLUSIONS: The high expression of E5 (-) E6 (-) is correlated with the poor prognosis for patients with APL.     

用α-半乳糖苷酶制备可供输注的人通用型O型红细胞

研究的目的是将人群中28％的B型血改造成通用O型血,提高O型血的储存和使用比例,以缓解战争、恐怖袭击、突发事件等紧急状态下对O型血的大量需求用α-1.3半乳糖苷酶作为B→O血型改造的工具酶,摸索改造一个使用单位B型血红细胞(100m1)的最佳条件,结果实现了在密闭、无菌条件下使用50U／ml工具酶．pH5．6,26℃ 2小时,进行B→O的血型改造。结论：改造后的通用型O型血红细胞符合生物制品检定规程的要求．并可在4℃存放21天     

三叉神经痛型桥小脑角胆脂瘤的临床分析

目的：探讨三叉神经痛型桥小脑角胆脂瘤的发生机制、临床表现和治疗原则。方法：24例患者中，18例CT平扫呈低密度，16例行MRI扫描，15例显示肿瘤T1加权像呈低信号，1例为混杂信号，T2加权像均为高信号，CT和MRI均无强化；17例瘤体实质直接包绕三叉神经，7例瘤体在三叉神经前内方将三叉神经压向后下方，3例使神经移位的同时，对侧小脑上动脉压迫三叉神经。结果：13例行肿瘤全切除，9例行肿瘤次全切除，遗留部分包膜未切，2例行大部切除。24例术后面部疼痛停止发作，随访1年以上疼痛未复发。结论：三叉神经痛型桥小脑角胆脂瘤CT和MRI扫描有特征性表现，应采取手术治疗，术中根据肿瘤和周围结构的关系以确定是否全切，对于同时受微血管压迫的病例，除行肿瘤切除外，同时行三叉神经微血管减压术，后多数预后良好。     

荧光定量PCR检测急性白血病患者外周血 WT1基因表达及其临床意义

为研究WT1基因表达与临床疗效和预后的关系,探讨其在白血病微小残留病检测中的作用,用荧光定量RT-PCR方法检测55例初发白血病患者外周血及10例正常人外周血的WT1基因表达,跟踪20例急性白血病患者外周血WT1基因表达。结果显示,白血病初治组(40例ANLL、15例ALL)与正常人外周血WT1基因表达有显著差异(P<0.001)。在急性白血病患者中,WT1≤6.8×10-3组生存期长于WT1>6.8×10-3组(P=0.027);白血病患者初发时WT1呈高度表达,完全缓解后,迅速或缓慢下降至少1个对数级,复发时再次增高。跟踪检测20例急性白血病患者外周血WT1基因表达,结果7例复发,5例在临床复发前2-3月WT1的水平明显增高,至少上升0.8个对数级。结论:荧光定量RT-PCR方法检测白血病外周血WT1表达具有简便易行、准确性高、特异性好的特点。与正常人外周血比较,WT1在各类白血病外周血中呈高度表达,且表达水平与预后负相关;对外周血WT1基因表达进行定量分析,可用于微小残留病的监测。