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71.
S ummary . Growth of human erythroid and erythroid-granulocytic colonies was investigated in a fibrinogen clot culture system. CFU-E, BFU-E, CFU-C and the mixed colonies were grown in cultures in medium conditioned by phytohaemagglutinin-stimulated human mononuclear cells, with small amounts of aplastic anaemia serum, but no exogeneous erythropoietin (Epo). The level of Epo in the culture was 0·015 U. The mixed colonies constituted 8-22% of the total of colonies grown. The results indicate that high dose Epo is not required to grow BFU-E and the mixed colonies in cultures containing erythroid potentiating factor(s), and the frequency of bipotent haemopoietic stem cells in human bone marrow or peripheral blood may be higher than that reported previously. 相似文献
72.
Jun?Ozaki Hirokazu?OkumuraEmail author Kouji?Iwamoto Shigeru?Shimadoi Shinichi?Yamanaka Shigeki?Ohtake Shinji?Nakao 《International journal of hematology》2004,79(1):95-99
Crow-Fukase syndrome (CFS) is a multisystemic disorder. Because it is characterized by polyneuropathy, organomegaly, endocrinopathy,
monoclonal gammopathy, sclerotic bone lesions, and skin changes it is also known as POEMS syndrome. Extravascular volume overload
is also one of the main symptoms. Uncontrollable extravascular volume overload is one of the major causes of death and one
of the negative prognostic factors. Control of the extravascular volume overload is an important therapeutic strategy for
this syndrome. We report here a case of CFS with extravascular volume overload resulting in pleural effusion and massive edema
in the lower extremities, which was refractory to oral administration of melphalan and prednisolone. The patient's condition
correlated with the serum level of vascular endothelial growth factor and markedly improved after administration of high-dose
melphalan (200 mg/m2) followed by autologous peripheral blood stem cell transplantation. This approach should be considered in patients with CFS
who fail to respond to conventional chemotherapy and have uncontrollable extravascular volume overload. 相似文献
73.
Kameda N Higuchi K Shiba M Machida H Okazaki H Yamagami H Tanigawa T Watanabe K Watanabe T Tominaga K Fujiwara Y Oshitani N Arakawa T 《Journal of gastroenterology》2008,43(6):434-440
BACKGROUND: Wireless capsule endoscopy (CE) and double-balloon enteroscopy (DBE) are new methods enabling diagnostic endoscopy of the entire small intestine. However, which of the two is superior is unclear. We therefore prospectively compared the clinical efficacy of CE and DBE. METHODS: We prospectively examined 32 patients with obscure gastrointestinal bleeding. CE preceded DBE by 1-7 days, and all patients underwent DBE twice, by antegrade and retrograde approaches, to evaluate the entire small intestine. Physicians evaluating the results of CE and DBE were blind to the results of the other method. We evaluated diagnosis, diagnostic yield of the two methods, and clinical outcomes. RESULTS: CE revealed abnormal findings in 29 (90.6%) of 32 patients. CE definitively or probably detected the sources of bleeding in 23 (71.9%) of the 32 patients, including angioectasias (eight), erosions (seven), ulcers (five), tumor (one), and hemorrhagic polyps (two). DBE definitely or probably detected the sources of bleeding in 21 (65.6%) of the 32 patients, including angioectasias (seven), erosions (four), ulcers (five), tumor (one), hemorrhagic polyps (two) and diverticula (two). CE yielded more abnormal findings than DBE (CE 90.6%, DBE 65.6%) (P = 0.032), although there were no significant differences in diagnostic yield between the methods. We were able to perform additional treatment or biopsy with DBE in 13 patients, including coagulation therapy (ten), endoscopic mucosal resection (one), biopsy (seven), and extraction of retained CE (two). CONCLUSIONS: Our results demonstrate the superiority of CE in detecting abnormal lesions, and the superiority of DBE in endoscopic management. 相似文献
74.
Yoshitaka T Abe N Minagawa H Date H Sakoma Y Nishida K Ozaki T 《Modern rheumatology / the Japan Rheumatism Association》2008,18(4):359-365
We prospectively evaluated the disease-specific features of the early postoperative plasma D: -dimer value and the relationship with deep venous thrombosis and/or pulmonary thromboembolism (DVT/PE) in 95 patients following total knee arthroplasty. Patients in whom DVT/PE was highly suspected were diagnosed by high-resolution multi-detector row computed tomography scanning (MDCT). Forty-nine knees in 46 patients with rheumatoid arthritis (RA, 24 knees) or osteoarthritis (OA, 25 knees) were finally recruited. DVT/PE was detected in 28 (57.1%) of the 49 cases examined by diagnostic MDCT: 12 (50.0%) of the 24 cases of RA, and 16 (64.0%) of the 25 cases of OA. Of these, PE was found in 11 cases (39.2%), but none of them showed clinical symptomatic signs of dyspnea or chest pain. In both RA and OA cases, there were statistically significant differences in the D: -dimer value on postoperative day 3 (P = 0.027) and after day 28 (P = 0.037) between the groups with and without DVT/PE. In OA cases, there were significant differences between the two groups on postoperative days 1 (P = 0.034), 3 (P = 0.020), 5 (P = 0.005), and 7 (P = 0.045), respectively. At the baseline, perioperative D: -dimer levels in the RA group without DVT/PE were higher than in the OA group. However, multivariate logistic regression analysis showed that RA was not a significant risk factor of DVT/PE in comparison with OA. In conclusion, individual evaluation of the D: -dimer level between RA and OA should provide a more precise predictive indicator of early postoperative DVT/PE. 相似文献
75.
Matsushima Y Kinoshita Y Fukui H Maekawa T Yazumi S Okada A Nakase H Kawanami C Iwano M Hashimoto K Takeda Z Okazaki K Chiba T 《Journal of gastroenterology》2002,37(6):428-433
Background:
Background: Although Helicobacter pylori eradication is effective in treating low-grade gastric mucosa-associated lymphoid tissue (MALT) lymphoma, the condition in
some patients deteriorates even after the eradication. Therefore, it is important to predict the disease outcome before starting
H. pylori eradication. We investigated the usefulness of flow cytometry, quantifying CD19- and CD20-positive B lymphocytes in MALT lymphoma
tissue, for predicting the disease outcome after H. pylori eradication.
Methods: Tissue specimens from 14 patients with H. pylori-positive low-grade gastric MALT lymphoma were examined by histology, Southern blotting, and flow cytometry before therapy.
Serum levels of soluble interleukin (IL)-2 receptor were also measured. The relationship between the data and the prognosis
after H. pylori eradication was analyzed.
Results: Remission occurred in 10 of the 14 patients. The condition in the 4 remaining patients deteriorated even after H. pylori eradication. The percentages of CD19- and CD20-positive cells in MALT lymphoma tissue from the patients in remission were
both significantly lower than those in the tissue from patients not in remission. Indeed, 4 of the 5 patients in whom both
CD19- and CD20-positive cells accounted for more than 50% of the total number of lymphocytes had gastrectomy, whereas all
patients in whom both CD19- and CD20-positive cells accounted for less than 50% of the total number of lymphocytes achieved
remission. Although immunoglobulin gene rearrangement was present in all patients operated on, there were also 6 patients
whose MALT lymphoma was ameliorated in spite of the presence of gene rearrangement. The serum level of soluble IL-2 receptor
was in the normal range in all patients tested.
Conclusions: Analysis of mature B-cell markers in MALT lymphoma tissue is more useful than the examination of immunoglobulin gene rearrangement
or serum levels of soluble IL-2 receptor in predicting the outcome of low-grade gastric MALT lymphoma after H. pylori eradication.
Received: January 5, 2001 / Accepted: November 2, 2001 相似文献
76.
Sato T Toki T Kanezaki R Xu G Terui K Kanegane H Miura M Adachi S Migita M Morinaga S Nakano T Endo M Kojima S Kiyoi H Mano H Ito E 《British journal of haematology》2008,141(5):681-688
JAK3 mutations have been reported in transient myeloproliferative disorder (TMD) as well as in acute megakaryoblastic leukaemia of Down syndrome (DS-AMKL). However, functional consequences of the JAK3 mutations in TMD patients remain undetermined. To further understand how JAK3 mutations are involved in the development and/or progression of leukaemia in Down syndrome, additional TMD patients and the DS-AMKL cell line MGS were screened for JAK3 mutations, and we examined whether each JAK3 mutation is an activating mutation. JAK3 mutations were not detected in 10 TMD samples that had not previously been studied. Together with our previous report we detected JAK3 mutations in one in 11 TMD patients. Furthermore, this study showed for the first time that a TMD patient-derived JAK3 mutation (JAK3(I87T)), as well as two novel JAK3 mutations (JAK3(Q501H) and JAK3(R657Q)) identified in an MGS cell line, were activating mutations. Treatment of MGS cells and Ba/F3 cells expressing the JAK3 mutants with JAK3 inhibitors significantly decreased their growth and viability. These results suggest that the JAK3 activating mutation is an early event during leukaemogenesis in Down syndrome, and they provide proof-of-principle evidence that JAK3 inhibitors would have therapeutic effects on TMD and DS-AMKL patients carrying activating JAK3 mutations. 相似文献
77.
Mari Terada Satoshi Kutsuna Tomiteru Togano Sho Saito Noriko Kinoshita Yumiko Shimanishi Tetsuya Suzuki Yusuke Miyazato Makoto Inada Takahito Nakamoto Hidetoshi Nomoto Satoshi Ide Mitsuhiro Sato Kenji Maeda Akihiro Matsunaga Masahiro Satake Keiji Matsubayashi Hirokazu Tsuno Makiko Kojima Madoka Kuramistu Kenta Tezuka Emi Ikebe Kazu Okuma Isao Hamaguchi Katsuyuki Shiratori Motohiko Sato Yuiko Kawakami Kumi Inaba Saori Igarashi Reina Yamauchi Mina Matsumura Keiko Ishimaru Bijuan Zhang Chika Kuge Maiko Ishihara Miho Gouda Keiko Tanaka Yukihito Ishizaka Norio Ohmagari 《Transfusion》2021,61(7):1998-2007
78.
Chieko Sakano Makoto Kuroda Tsuyoshi Sekizuka Taisei Ishioka Yukio Morita Akihide Ryo Hiroyuki Tsukagoshi Yuko Kawai Nobuko Inoue Hayato Takada Yumiko Ogaswara Atsuyoshi Nishina Masa-aki Shimoda Kunihisa Kozawa Kazunori Oishi Hirokazu Kimura 《Journal of clinical microbiology》2013,51(1):328-330
Whole-genome sequencing of non-H2S-producing Salmonella enterica serovar Typhimurium and S. enterica serovar Infantis isolates from poultry meat revealed a nonsense mutation in the phsA thiosulfate reductase gene and carriage of a CMY-2 β-lactamase. The lack of production of H2S might lead to the incorrect identification of S. enterica isolates carrying antimicrobial resistance genes. 相似文献
79.
Takeshi Saraya Michiaki Mikoshiba Harumi Kamiyama Masakazu Yoshizumi Shigeru Tsuchida Hiroyuki Tsukagoshi Taisei Ishioka Miho Terada Eiichi Tanabe Chizuko Tomioka Haruyuki Ishii Hirokazu Kimura Kunihisa Kozawa Tetsuo Shiohara Hajime Takizawa Hajime Goto 《Journal of clinical microbiology》2013,51(6):1979-1982
The present case provides direct evidence of human herpesvirus 6 reactivation in resected lymph node tissue in a patient with drug-induced hypersensitivity syndrome. This case clearly demonstrates that appropriate pathological evaluation of lymphadenopathy for drug-induced hypersensitivity syndrome, which mimics malignant lymphoma in clinical, radiological, and pathological findings, is required. 相似文献
80.
Tanya L. Brown Hirokazu Hashimoto Lisbet T. Finseth Teresa L. Wood Wendy B. Macklin 《The Journal of neuroscience》2021,41(9):1864
The actin cytoskeleton is crucial for oligodendrocyte differentiation and myelination. Here we show that p21-activated kinase 1 (PAK1), a well-known actin regulator, promotes oligodendrocyte morphologic change and myelin production in the CNS. A combination of in vitro and in vivo models demonstrated that PAK1 is expressed throughout the oligodendrocyte lineage with highest expression in differentiated oligodendrocytes. Inhibiting PAK1 early in oligodendrocyte development decreased oligodendrocyte morphologic complexity and altered F-actin spreading at the tips of oligodendrocyte progenitor cell processes. Constitutively activating AKT in oligodendrocytes in male and female mice, which leads to excessive myelin wrapping, increased PAK1 expression, suggesting an impact of PAK1 during active myelin wrapping. Furthermore, constitutively activating PAK1 in oligodendrocytes in zebrafish led to an increase in myelin internode length while inhibiting PAK1 during active myelination decreased internode length. As myelin parameters influence conduction velocity, these data suggest that PAK1 may influence communication within the CNS. These data support a model in which PAK1 is a positive regulator of CNS myelination.SIGNIFICANCE STATEMENT Myelin is a critical component of the CNS that provides metabolic support to neurons and also facilitates communication between cells in the CNS. Recent data demonstrate that actin dynamics drives myelin wrapping, but how actin is regulated during myelin wrapping is unknown. The authors investigate the role of the cytoskeletal modulator PAK1 during differentiation and myelination by oligodendrocytes, the myelinating cells of the CNS. They demonstrate that PAK1 promotes oligodendrocyte differentiation and myelination by modulating the cytoskeleton and thereby internode length, thus playing a critical role in the function of the CNS. 相似文献