四种含淫羊藿中成药中淫羊藿甙的含量测定

本文采用高效液相色谱法测定了四种中成药中淫羊藿甙的含量。以十八烷基硅烷键合硅胶为填料，柱温３０℃；甲醇－０．２ｍｏｌ／Ｌ磷酸二氢钠水溶液（磷酸调ｐＨ＝２．７）（５７：４３）为流动相，１ｍｌ／ｍｉｎ；检测波长为２６８ｎｍ，淫羊藿甙色谱峰形对称、分离度好。所测补肾强身片、龟龄集、壮骨关节丸和前列宁冲剂中淫羊藿甙的含量分别为０．１０５％，０．０９９５％，０．０５６６％，０．３１３％。     

EXPERIMENTAL STUDY OF RADIOIMMUOGUIDED SURGERY IN OVARIAN CARCINOMA

INTRODUCTIONThemaintreatmenttodateforovariancarcinomaissurgicalresection,thesocalledcytoreductivesurgery.Toperformacompleteresection,surgeonsusuallydependonvisualandtactilesensesaswellassurgicalinstinctswhicharerelativelycrudeinidentifyingsubclinicalmicroscopictumor.Radioimmuno--guidedsurgerysystem(RIGS)hasbeentakenoverthelast10yearsinbothlaboratoryandclinicalsettings.However,onlyafewreportswereseenaboutitsstudyinovariancancerworldwideespeciallyinourcountry.OurstudyincludeestablishingaR…     

癌旁组织CD44vmRNA的表达对原发性肝癌术后复发的影响

研究原发性肝癌癌旁组织ＣＤ４４ｖｍＲＮＡ表达的意义。应用ＲＴ－ＰＣＲ检测原发性肝癌癌旁组织ＣＤ４４ｖｍＲＮＡ的表达并结合术后近２年的随访结果进行综合分析。癌旁组织ＣＤ４４ｖｍＲＮＡ表达高于癌组织者的肿瘤包膜缺如、门静脉癌栓和瘤周卫星结节等与转移相 关的病理指标高于癌旁组织ＣＤ４４ｖｍＲＮＡ表达低于癌组织的病例，前者复发率亦高于后者。结论：癌旁组织ＣＤ４４ｖｍＲＮＡ的表达肝癌侵袭潜能以及预测其复发等     

2型糖尿病患者外周血白细胞iNOSmRNA表达的变化及意义

目的研究2型糖尿病DM患者外周血白细胞中iNOSmRNA表达的变化及其与糖尿病肾病DN发生、发展的关系。方法101例2型DM患者,根据尿微量白蛋白排泄率和血肌酐水平分为单纯DM组和不同的DN组,用原位杂交法检测外周血白细胞iNOSmRNA表达的阳性细胞的百分率,并与21例健康体检者进行比较。结果早期DN组白细胞iNOSmRNA表达的百分率明显高于对照组、DM组及晚期DN组(P<0.001)。结论外周血白细胞iNOSmRNA表达的变化参与了DN的发生、发展。     

Potential contribution of nuclear factor-kappaB to cerebral vasospasm after experimental subarachnoid hemorrhage in rabbits.

Nuclear factor-kappaB (NF-kappaB) plays a key role in inflammation, which is involved in the development of cerebral vasospasm after subarachnoid hemorrhage (SAH). In the present study, we assessed the potential role of NF-kappaB in regulation of cerebral vasospasm. Nuclear factor-kappaB DNA-binding activity was measured in cultured vascular smooth muscle cells (VSMCs) treated with hemolysate and pyrrolidine dithiocarbamate (PDTC, 80 micromol/L), an inhibitor of NF-kappaB. Forty-two rabbits were divided into three groups: control, SAH, and PDTC groups (n=14 for each group). The caliber of the basilar artery was evaluated. Nuclear factor-kappaB DNA-binding activity and the gene expression levels of cytokines and adhesion molecules in the basilar artery were measured. Immunohistochemical study was performed to assess the expression and localization of tumor necrosis factor (TNF)-alpha, intercellular adhesion molecule (ICAM)-1, and myeloperoxidase (MPO). It was observed that NF-kappaB DNA-binding activity was significantly increased by treatment with hemolysate in cultured VSCMs, but this increase was suppressed by pretreatment with PDTC. Severe vasospasm was observed in the SAH group, which was attenuated in the PDTC group. Subarachnoid hemorrhage could induce increases of NF-kappaB DNA-binding activity and the gene expression levels of TNF-alpha, interleukin (IL)-1 beta, ICAM-1, and vascular cell adhesion molecule (VCAM)-1, which were reduced in the PDTC group. Immunohistochemical study demonstrated that the expression levels of TNF-alpha, ICAM-1, and MPO were all increased in the SAH group, but these increases were attenuated in the PDTC group. Our results suggest that NF-kappaB is activated in the arterial wall after SAH, which potentially leads to vasospasm development through induction of inflammatory response.     

凋亡敏感基因在短暂性缺氧再复氧复注血清后的大鼠星形胶质细胞中的表达及其意义

目的研究一种新发现的抗氧化蛋白质--凋亡敏感基因(SAG)在短暂性缺氧再复氧复注血清诱导的细胞坏死和凋亡中的作用.方法用短暂性缺氧再复氧复注血清来诱导原代培养的大鼠大脑皮质星形胶质细胞损伤,用免疫细胞化学方法检测凋亡敏感基因的表达,并作图像分析;用流式细胞仪检测胶质细胞在短暂缺氧再复氧复注血清后不同时间点的凋亡率.结果凋亡敏感基因在缺氧15 min再复氧复注血清5 h后表达最高,在复氧复注血清16 h后恢复至对照组水平;细胞凋亡率在缺氧15 min再复氧复注血清1h时达到最高,而在再复氧复注血清5 h后降至对照组水平.结论凋亡敏感基因具有抗凋亡的作用,可减轻星形胶质细胞的缺血再灌注损伤.     

腹腔镜与开腹胆囊切除胃肠压力变化的临床研究

目的 :从胃肠道压力变化的角度探讨腹腔镜与开腹胆囊切除对胃肠运动功能的影响。方法 :腹腔镜胆囊切除 30例 (男 6例 ,女 2 4例 ,4 7± 4岁 ) ,开腹胆囊切除 30例 (男 9例 ,女 2 1例 ,4 7± 7岁 ) ,分别于手术前 1d行胃电图描记 ,记录术后 3、2 4、4 8、72h胃电图及监测胃窦、十二指肠和空肠压力 (移行性运动复合波 ,MMCⅢ )。结果 :(1)手术前腹腔镜和开腹胆囊切除患者胃电频率差异无显著性 (P >0 .0 5 ) ;(2 )腹腔镜胆囊切除组术后 3、2 4h正常波所占百分比低于术前 ,但差异无显著性 (P >0 .0 5 ) ,术后 4 8h恢复正常 ;(3)开腹胆囊切除组术后起 3h正常波所占百分比明显低于术前 ,差异有显著性 (P <0 .0 1) ,术后 2 4、4 8h正常波的百分比与术前差异无显著性 ,术后 72h恢复正常 ;(4) 2组患者术后胃窦、十二指肠及空肠压力低于健康人群 (P<0 .0 5 ) ;(5 ) 2组胃窦部收缩压力及收缩曲线下面积术后第 1、2、3d较术后 3h明显升高 (P <0 .0 5 ) ,且随时间延长有逐渐增大的趋势 ,腹腔镜组术后 72h恢复正常 ,十二指肠及空肠术后 3d内无明显变化 ;(6 ) 2组间胃电频率 ,胃窦部、十二指肠及空肠压力变化差异无显著性 (P >0 .0 5 ) ,但显示有差别。结论 :腹腔镜与开腹胆囊切除术均可引起胃电频率及胃肠压力变化 ,开     

幕上星形细胞肿瘤MRI表现与VEGF、P16、Ki-67相关性研究

探讨星形细胞肿瘤MRI表现与P16、Ki—67、血管内皮生长因子(vascular endothelial growth factor，VEGF)表达的相关性。材料和方法：收集我院术前经MRI诊断并经手术病理证实的幕上星形细胞肿瘤53例，行常规平扫描后静脉注射Gd—DTPA增强成像；同时采用HE染色及链菌素生物素—过氧化酶连接法进行VEGF、Ki—67、P16的免疫组织化学染色，测定它们的表达指数。结果：星形细胞肿瘤的信号不均匀性、坏死、水肿、占位效应、强化程度与VEGF、Ki—67、P16的表达相关，且随着星形细胞肿瘤恶性程度的增高VEGF、Ki—67的表达增加；P16的表达率降低。结论：星形细胞肿瘤的MRI表现与VEGF、Ki—67、P16的表达密切相关，MRI可以间接判断星形细胞肿瘤的某些生物学行为。     

The liver first pass effects and bile concentrations of (-)-, (+)- and (+/-)-praziquantel in rabbits

Praziquantel (PQT) is a racemic mixture. Intragastric gavage of (-)-, (+)- and (+/-)-PQT 100 mg/kg were used to study the liver first pass effects in rabbits. HPLC was used to determine the concentration of each compound in the portal and systemic circulation, and in the bile within 4h. The results showed that all drugs were absorbed rapidly from the gut into the portal vein. The serum concentration in the portal vein were similar. However, over 90% of PQT and its enantiomers were extracted in the first passage through the liver. Intrinsic metabolic clearances of the liver of (-)-, (+)- and (+/-)-PQT were 50.3, 174.4 and 52.6 L/h, respectively. The serum concentrations of the three drugs markedly decreased in the systemic circulation, especially that of (+)-PQT. The AUC of (+)-PQT was apparently lower than that of (-)- or (+/-)-PQT. From these results, it is assumed that the first pass effects of PQT and its enantiomers in the liver are pronounced and most likely stereoselective. Also, unchanged PQT and its enantiomers were found in the bile of the rabbits.     

乳母血清泌乳素水平与泌乳量关系的观察

用放射免疫法测定产后40天和60天乳母血清泌乳素基础水平和吸吮后泌乳素最大反应性增值,发现其与乳母泌乳量、婴儿吸吮频率、吸吮时间之间均不存在正相关关系,推测引起泌乳素分泌除吸吮外,还存在其他机理。