Agilent 2100 Bioanalyzer在人乳头瘤病毒检测中的应用

目的：探讨Agilent2100Bioanalyzer芯片分析系统（简称Bioanalyzer)在人乳头瘤病毒（HPV）的PCR检测和分组中的应用。方法：先分别进行通用引物介导PCR（GP－PCR）和型特异性引物介导PCR，扩增HPV高保守区（L1区）的共同序列和各型（包括HPV6，11，16和18型）的特型性序列，然后分别用常规的琼脂糖凝胶电泳技术和Bioanalyzer对PCR产物进行检测，并比较两种检测方法的准确度，重复性和灵敏度。结果：Bioanalyzer在确定片段长度时的准确度和重复性比琼脂糖凝胶电泳高，前者的准确度在95％以上，后者仅为85％，Bioanalyzer的检测灵敏度比琼脂糖凝胶电泳高100倍，结论：Bioanalyzer芯片分析系统结合PCR方法可对HPV进行特异，准确，稳定，灵敏的检测和型别鉴定，具有重要的推广价值。     

中西医结合治疗糖尿病周围神经病变疗效观察

29例非胰岛素依赖型糖尿病合并周围神经病变患者分成两组:一组为控制血糖组共12例,经8个月严格控制血糖,正中、尺、腓运动神经传导速度和正中、尺感觉神经潜伏期获改善,而且改善的程度与空腹血糖和糖基化血红蛋白降低的程度密切相关;另一组为针灸加控制血糖组共17例,经1与3个月的治疗,分别有82.4%和90.0%的患者神经病变临床症状得到改善,但正中、尺、腓运动神经传导速度和正中、尺感觉神经潜伏期未得到改善。     

COMPARATIVE STUDY OF AMINO ACID CONTENTS AND LDH ISOZYME BY ELECTROPHORESIS AND SLAB-PAGE IN ADULT WORMS OF ASCARIS LUMBRICOIDES, ASCARIS SUUM AND TOXOCARA CANIS^

Comparative studies are made on the relative percentages of amino acid content and LDH isozyme by electrophoresis and Slab-PAGE in adult worms of Ascaris lumbricoides, Ascaris suum and Toxocara canis. The results show that there are remarkable differences of Arg and His of 17 amino acids bet. ween Toxocara canis and human intestinal ascaris, human biliary ascaris and pig ascaris; there are also obvious differences of band positions of LDH isozyme electrophoretic patterns and band position and amount of slab-PAGE patterns among them, whereas no marked differences are found in these three indices in human intestinal ascaris, human biliary ascaris and pig ascaris.     

维生素D的骨外作用

越来越多的研究表明,维生素D除具有经典的调节钙磷平衡和维持骨骼健康的作用外,还具有其他更为广泛的骨外生物学效应,如调节免疫、抗肿瘤、保护中枢神经系统和防治代谢综合征等作用.本文就相关方面的研究进展进行综述.     

睾丸微石症2例报告及文献复习

目的:认识睾丸微石症(TM)的临床意义和诊断方法。方法:回顾性报告2例TM病例的临床资料,结合文献进行讨论。结果:2例分别因左精索静脉曲张和附睾炎就诊的患者行阴囊超声检查时发现睾丸内散在多发点状强回声,如针尖样,1～2mm,后无声影。分别诊断为局部TM、左精索静脉曲张和TM,左附睾炎。甲胎蛋白、人绒毛膜促性腺激素、乳酸脱氢酶和睾酮等检查均正常。对睾丸微石症未做特殊处置,随访6～8个月,体检和睾丸瘤标未见异常,阴囊超声睾丸图像无特征性改变。结论:TM是一种少见、无明显临床症状、相对稳定的疾病。多因阴囊其他疾病行超声检查时偶然发现,其是否属于癌前病变尚有争议,但是定期随访和阴囊超声检查是必要的。     

氧化苦参碱抑制人肝癌细胞EGF和EGFR表达的研究

目的:探讨氧化苦参碱对人肝癌细胞EGF及EGFR表达的影响。方法:将肝癌细胞株BEL-7402在37℃、5%CO2条件下培养、传代。按氧化苦参碱浓度分为1000μg/ml、500μg/ml、250μg/ml3组,对照组不加氧化苦参碱,每组设6个平行孔,每孔加入细胞1×105/ml,培养24h、48h和72h,分别进行EGF及EGFR的免疫组化染色。结果:不同浓度的氧化苦参碱均抑制EGF和EGFR在人肝癌细胞的表达,随着培养时间的延长,EGF和EGFR表达阳性率下降显著,在1000μg/ml组24h、48h和72h的EGF和EGFR阳性率均较500μg/ml组和250μg/ml组同时段的阳性率下降显著(P〈0.05,P〈0.01)。500μg/ml组和250μg/ml组同时段的阳性率无明显差异。结论:氧化苦参碱对EGF和EGFR的抑制途径可能是其抗肝癌机制之一。     

Intrathecal landiolol inhibits nociception and spinal c-Fos expression in the mouse formalin test

PURPOSE: The purpose of this study was to determine if intrathecal landiolol, a beta1-blocker, can modulate formalin-induced nociception and spinal c-Fos expression in mice, in the absence of anesthesia. METHODS: Thirty-two mice were randomly assigned to one of four groups: the control group (n = 8) received intrathecal normal saline 10 microL, while the other three groups (n = 8 for each) received intrathecal landiolol at escalating doses of 250 microg.kg(-1), 500 microg.kg(-1) and 750 microg.kg(-1) respectively, immediately after induction of anesthesia with isoflurane. After awakening, inflammatory pain was induced by 10 microL of 5% formalin solution injected into the dorsal surface of the right hind paw. The nociceptive behaviours including licking, biting and lifting of the injected paw were cumulatively recorded as seconds of behaviours/min during phase I (0-10 min) and phase II (10-45 min). The c-Fos protein expressions in the spinal dorsal horn were detected with immunohistochemical techniques in the control and landiolol 750 microg.kg(-1) groups. RESULTS: Compared to the control group, intrathecal injection of landiolol 750 microg.kg(-1) significantly decreased pain-related behaviours in phase I, while intrathecal landiolol 250 microg.kg(-1), 500 microg.kg(-1) and 750 microg.kg(-1) significantly decreased pain-related behaviours in phase II during the formalin test. The numbers of c-Fos immunoreactive nuclei in the L5 spinal dorsal horn were significantly lower in the landiolol 750 microg.kg(-1) group compared to the control group (landiolol 750 microg.kg(-1) 2.4 +/- 1.1 vs control 9.2 +/- 3.9; P < 0.01). CONCLUSION: The present study indicates that intrathecally administered landiolol produces significant antinociceptive effects in the formalin test. Although further studies exploring the detailed mechanism are needed, these data suggest a potential role of beta1-adrenoreceptors in spinal nociceptive processing.     

雷帕霉素对炎性反应状态下肾小球系膜细胞内脂质稳态的影响

Objective To investigate the effect of rapamycin on cholesterol homeostasis of glomerular mesangial cells and the underlying mechanism. Methods Glomerular mesangial cell line (HMCL) cells were cultured and divided into control group, IL-1β group and different concentration rapamycin groups. Intracellular cholesterol accumulation was observed and measured by oil O staining and HPLC. Real-time quantitative PCR and Western blot were used to detect the mRNA and protein expression of LDLR, PPARγ, LXRα, ABCA1 in HMCL after the treatment with IL-1β and rapamycin. Results Rapamycin had no significant influence on intracellular cholesterol concentration under normal condition. IL-1β significantly increased the intracellular cholesterol concentration by 143％ of control (P<0.05), and 10, 50, 100 ?滋g/L rapamycin could significantly inhibit the effect of IL-1β (87％, 116％, 96％ of control respectively, all P<0.05). Rapamycin could suppress the increased expression of LDLR caused by IL-1β on both mRNA and protein level in a dose-dependent manner（P<0.01）. Rapamycin dose-dependently up-regulated the reduced mRNA and protein expression of ABCA1, the decreased mRNA expression of PPARγ and LXRα induced by IL-1β as well (P<0.01）. Conclusion Rapamycin may contribute to the maintenance of intracellular cholesterol homeostasis in glomerular mesangial cells under inflammatory state by both reducing cholesterol uptake and promoting cholesterol efflux.     

谷胱甘肽- S-转移酶π和 DNA拓扑异构酶Ⅱ-α在结直肠癌组织中的表达及其意义

目的探讨谷胱甘肽- S-转移酶π （GST-π ）和 DNA拓扑异构酶Ⅱ-α （Topo Ⅱ-α ）在肿瘤的表达及其与结直肠癌临床病理学特征之间的关系.方法应用免疫组织化学（免疫组化） ABC法检测 GST-π和 Topo Ⅱ-α在 60例结直肠癌、癌旁组织和 15例正常结肠黏膜中的表达并分析其意义.结果 GST-π和 Topo Ⅱ-α在结直肠癌组织中的阳性表达率分别为 90.0%和 86.7%,显著高于癌旁组织和正常黏膜（ P〈 0.01）. GST-π和 Topo Ⅱ-α表达强度与肿瘤大小和组织学类型无关（ P 〉0.05）,但与肿瘤的临床分期、分化程度和淋巴结转移与否密切相关（ P〈 0.01）. GST-π在低分化癌组织中高表达;Topo Ⅱ-α在高分化癌组织中的表达强度高于低分化癌（ P〈 0.01）.结论 GST-π和 Topo Ⅱ-α在结直肠癌组织中的表达与肿瘤的临床病理特征有关,可作为衡量肿瘤恶性程度和预后的指标之一.