99mTc-DTPA气溶胶显像测定呼吸道粘液纤毛清除功能

为进一步研究呼吸道疾病时的粘液纤毛清除功能,采用99mTc-DTPA气溶胶吸入显像,通过电影显示定性和定量指标分析.对18 例健康受试者和患者进行了显像研究,得到气道清除率和粘液纤毛清除率的正常值,并观察了32例慢性阻塞性肺病(COPD)患者气管-支气管纤毛清除功能的改变.结果显示,健康受试者与COPD患者粘液纤毛清除率(mm/min)分别为3.89±0.92和1.32±0.59,两者比较有显著性差异(P＜0.01),COPD患者不同时间气道清除率和粘液纤毛清除率均明显低于健康受试者(P＜0.01).本研究方法简便客观,具有定性观察和定量分析的优点,对于呼吸系统其它疾病(如支气管扩张、原发性纤毛无运动)的研究及药物疗效的评价具有重要的临床实用价值.     

重组人肝细胞生长因子基因的克隆

用ＲＴ－ＰＣＲ技术从人胎盘组织内成功地扩增全长肝细胞生长因子（ＨＧＦ）ｃＤＮＡ基因（２ １８４ｂｐ）,并将其克隆至ｐＧＥＭ－Ｔ载体,经限制性核酸内切酶ＮｄｅⅠ,ＢｇｌⅡ,ＨｉｎｄⅢ,ＢａｍＨⅠ和ＸｈｏⅠ的酶谱分析和ＤＮＡ测序分析证实。再将其亚克隆至逆病毒载体ｐＬＮＬ－ＸＨＣ,可进一步用于基因表达和基因治疗的研究。     

医古文求疵四则

古人用字行文,决非凭空虚构;遣词造句,十分精确.反复阅读,才能体味其中之妙.本义、引申,需谋全篇,方能准确理解.笔者不揣愚陋,就医古文规划教材的注释提出求疵四则,以便投石问路.     

Studies of β-lactamase production in ampicillin resistant Haemophilus influenzae

In 1972, HaelnOPhilus influenzae (HI) was firstlydiscovend being resistant against alnpicillin by producing5-lac~e, since then, studies on the rests~ mechamsms and the moleCular basis of 5-lactalnaSe Pwhctionhave been increasing year by yeax abroadll]. In 1978,Bryan et .l[2] first reported sabins of HI Producing 0-lactamaSe, which was mediated by plaaed, from then on,many stUdies foeused on 3-lactamase encoded by PlaSnddin HI and its relation with ampicillin resisboce. In ourco~, few st…     

Role of Th1/Th2 cells activation in chronic hepatitis B virus infection

Clonal analysis of mndne T cells has led to the idenuncation of Z typeS of Th cells, Thl and on. Thl cells,chacterized by secreting interferon-y (Iap-Y), interleukin-2 (IL2) and tumor necrosis factor-5 (TNF-3), involved Principally in cell-mediated ~ity and play acrucial role in defending the host against intracellularPathogens such as a Vallety of yi~es. ThZ cells,secreting IL4, ms, IL-6 and ilo, mostly regulateh~ immune responSe, and may oafs Paxtial PIDtection against extracellula…     

奥曲肽治疗肝硬变食管静脉曲张破裂出血

0　引言　我院 1996 - 0 6 / 1998- 0 7用奥曲肽治疗肝硬变食管静脉曲张破裂出血 19例 ,并与垂体后叶素作了对照 .1　对象和方法1.1　对象　经内镜证实食管静脉曲张破裂出血 37例 ;男 32例 ,女 5例 ;年龄 42～ 6 9岁 .肝炎后肝硬变 32例 ,乙醇性肝硬变 3例 ,血吸虫性肝硬变 2例 .按就诊顺序随机分为治疗组19例 ,对照 18例 .估计出血量 :治疗组 (146 3.6± 375 .8) m L;对照组 (144 9.5± 36 8.4) m L .出血至治疗间隔时间 :治疗组(11.6± 3.5 ) h;对照组 (11.2± 3.6 ) h.两组的年龄、性别、肝功 Child分级、食管静脉曲张程度、出血量、出…     

药物诱导狗Ⅰ型糖尿病的研究

To evaluate the efficiency and toxic and side effect of the combined chemical inducer for Type I diabetes mellitus, Type I diabetes in 9 adult dogs were induced with alloxan(50 mg.kg-1) and streptozotocin (30 mg.kg-1) with vein injection. After 7-14 days, the blood glucose of 5 male dogs was kept on high levels [mean value was (22.7 +/- 3.2) mmol.L-1], exogenous insulin was (21.4 +/- 2.4) U.day-1. Three female dogs died in 7 days after they had been injected with the combined inducers. Alloxan-streptozotocin combination administration reduced the dosage of each drug, and decreased the toxic and side effect of each drug. The results suggest that this method has a high rate of success to induce Type I diabetes male dogs.     

Transcriptome analysis of Armillaria gallica 012 m in response to auxin

Gastrodia elata is an achlorophyllous and fully mycoheterotrophic orchid which obtains carbon and other nutrients from Armillaria species in its life cycle. Many researchers suggested that plant hormones, as signing molecules, play a central role in the plant–fungi interaction. In the process of Armillaria gallica 012 m cultivation, both exogenous indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) distinctly stimulated the growth of mycelia in solid media. The differential expression genes (DEGs) of A. gallica 012 m with IAA versus blank control (BK) and IBA versus BK were investigated. The results showed that more than 80% of DEGs of the IAA group were coincident with the DEGs of the IBA group, and more than half of upregulated DEGs and most of the downregulated DEGs of the IAA group coincided with those DEGs of the IBA group. Above research implied that A. gallica 012 m could perceive IAA and IBA, and possess similar responses and signaling pathways to IAA and IBA. The overlapping differential genes of the IAA group and IBA group were analyzed by GO term, and the results showed that several DEGs identified were related to biological processes including positive regulation of the biological process and biological process. The downregulated NmrA-like and FKBP_C genes might be benefit to the growth of mycelia. Those results can explain that exiguous IAA and IBA improved the growth of A. gallica to some extent. We speculate that IAA and IBA are signaling molecules, and regulate the expression of growth-related genes of A. gallica 012 m by the same signaling pathway.     

Human papillomaviruses and papillomatosis lesions of the female lower genital tract

Objective: The objective of this study was to determine whether human papillomavirus (HPV) infections are involved in the development of papillomatosis lesions of the lower female genital tract.Methods: A total of 616 biopsy specimens of genital papillomatous lesions (307 nodular and 309 papular types) from 598 patients were anaylyzed for the presence of HPV DNA sequences by polymerase chain reaction (PCR). These specimens were also examined by histopathological assessment for characteristic HPV-associated cytological changes, by immunohistochemical staining for HPV-associated antigen, and by electron microscopy for the presence of virions.Results: HPV DNA sequences were found in 97.9% (140 of 143 cases) and 1.1% (1 of 91 cases) of the nodular and papular papillomatosis cases tested, respectively. In 18 patients who had both types of papillomatosis lesions, HPV DNA was invariably found only in nodular tissues. HPV-associated antigen, koilocytosis, and virions were found in 53.6% (98 of 183 cases), 70.5% (129 of 183 cases), and 5.9% (5 of 85 cases) of nodular papillomatosis lesions tested, respectively.Conclusions: These data suggest that nodular papillomatosis was closely associated with HPV infection, but that papular papillomatosis of the lower female genital tract may have an etiology other than HPV infection.