Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine

Background

Asthma is one of the most common allergic diseases. Our previous studies have reported that FIP-fve in acute allergic mouse model can reduce inflammation, improve the balance of the Th1/Th2 system. However, the effects of reducing airway remodeling on FIP-fve is still unknown.

经尿道前列腺汽化术治疗合并心脑血管疾病的良性前列腺增生

目的探讨经尿道前列腺汽化术(TUVP)治疗合并心脑血管疾病的良性前列腺增生(BPH)患者的安全性及有效性。方法应用TUVP治疗合并心脑血管疾病的BPH患者63例。灌注液常为5%甘露醇,肾功能不全患者使用5%葡萄糖。如腺体小于或等于75g,则彻底切除;如腺体大于75g,年龄大于80岁,则作前列腺部分切除术。行国际前列腺症状评分(I-PSS),观察手术效果,评价手术安全性。结果该组63例患者均安全耐受手术。手术时间28～104min,平均52min。3～5d后拔除导尿管后排尿通畅。术后3个月I-PSS平均11.8分,最大尿流率(Qmax)平均16.5mL/s,与术前(I-PSS平均30.3分,Qmax平均6.5mL/s)相比差异均具有显著性(P<0.01)。2例行膀胱颈修切时出现包膜穿孔,发生电切综合征(TURS)先兆3例均及时处理,1例脑梗死患者,因长期服用抗凝药,术后出血行二次TUVP。结论TUVP是治疗合并心脑血管疾病的BPH患者安全有效的手术方式。     

Study on quantitative diagnosis model of TCM syndromes of post-stroke depression based on combination of disease and syndrome

Background:Post-stroke depression (PSD) is one of the most common stroke complications with high morbidity. Researchers have done much clinical research on Traditional Chinese Medicine (TCM) treatment, but very little research on diagnosis. Based on the thought of combination of disease and syndrome, this study will establish a unified and objective quantitative diagnosis model of TCM syndromes of PSD, so as to improve the clinical diagnosis and treatment of PSD.Objective:First: To establish a unified and objective quantitative diagnosis model of TCM syndromes in PSD under different disease courses, and identify the corresponding main, secondary, and concurrent symptoms, which are based on the weighting factor of each TCM symptom. Second: To find out the relationship between different stages of PSD and TCM syndromes. Clarify the main syndrome types of PSD under different stages of disease. Reveal the evolution and progression mechanism of TCM syndromes of PSD.Methods and analysis:This is a retrospective study of PSD TCM diagnosis. Three hundred patients who were hospitalized in the First Teaching Hospital of Tianjin University of TCM with complete cases from January 2014 to January 2019 are planned to be recruited. The study will mainly collect the diagnostic information from the cases, find the related indicators of TCM and Western medicine in PSD, and clarify the relationship between different disease stages and TCM syndromes. Finally, the PSD TCM syndrome quantitative diagnosis model will be established based on the operation principle of Back Propagation (BP) artificial neural network.Conclusion:To collect sufficient medical records and establish models to speed up the process of TCM diagnosis.     

SREBP-1/小凹蛋白1介导烟酸姜黄素酯对ApoE－/－小鼠的抗动脉粥样硬化作用

目的探寻烟酸姜黄素酯对动脉粥样硬化病变的作用及机制。方法50只7周龄ApoE^－/－小鼠,随机分为五组,即对照组、高脂组、辛伐他汀组［5mg/（kg·d）］、烟酸姜黄素酯低剂量组［33mg/（kg·d）］和烟酸姜黄素酯高剂量组（99mg/（kg·d）］,其中对照组给予普通饲料,其他组给予高脂饲料喂养。连续干预6周之后处死动物,检测血清中血脂水平;油红O染色法和HE染色法观察小鼠主动脉斑块及肝脏脂质蓄积;ELISA法检测血清中炎症因子肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）水平;Westernblot法检测小鼠肝脏中小凹蛋白1和SREBP-1的表达。结果与对照组相比,高脂组血清中总胆固醇（TC）和低密度脂蛋白胆固醇（LDLC）升高,高密度脂蛋白胆固醇（HDLC）降低,主动脉粥样硬化斑块增多。与高脂组相比,烟酸姜黄素酯组小鼠血清中的TC和LDLC明显降低,TNF-α和IL-6水平下降,而主动脉粥样硬化斑块减轻,肝脏脂质减少;烟酸姜黄素酯组肝脏小凹蛋白1蛋白表达高于高脂组,而SPEBP-1蛋白表达低于高脂组。结论烟酸姜黄素酯预防性给药能抑制高脂喂养所致的ApoE^－/－小鼠动脉粥样硬化斑块的形成;机制可能与减少SREBP-1、增加小凹蛋白1蛋白水平,调节肝脏脂质代谢及炎症反应有关。     

盆腔恶性肿瘤术后输尿管下段梗阻的腔镜手术治疗

背景与目的:盆腔恶性肿瘤手术后组织粘连、瘢痕形成或放射治疗等常导致输尿管下段受压梗阻及肾积水.本研究旨在评价利用腔镜技术治疗此类输尿管梗阻的疗效与安全性.方法:1998年1月至2007年3月,46例有输尿管下段梗阻伴盆腔肿瘤史的患者在中山大学附属第三医院接受了腔镜手术,分析嗣手术期及随访资料.结果:46例患者中,25例行腹腔镜输尿管-膀胱吻合术,18例行输尿管镜放置支架管引流,3例行经皮肾造瘘术,未发生严重并发症.平均手术时间82.5 min(30～140 min),术中出血45.5 ml(5～180 m1),均未接受输血.中位随访时问18.2个月(3个月～6.5年).术后3个月,静脉尿路造影及B超提示39例(84.8%)肾分泌恢复正常,其余7例(15.2%)肾积水减轻、肾功能改善;核素扫描提示平均患侧肾小球滤过率比术前升高(37.6 ml/min vs.21.3 ml/min,P＜0.05).所有输尿管-膀胱吻合口无狭窄.结论:腔镜手术治疗部分类型盆腔肿瘤手术后或放疗后下段输尿管梗阻是有效、可行的.     

Case selection and implementation of tubeless percutaneous nephrolithotomy

BackgroundThe tubeless percutaneous nephrolithotomy (PCNL) was proposed to eliminate the side effects of the nephrostomy tube in recent years, such as pain, channel infection, postoperative bleeding, and longer hospital stay. But there is neither clinical guidelines nor consensus about tubeless PCNL in clinical practice. The study is aimed to how to implement the tubeless PCNL step by step, including case selection preoperatively, improving the technique of the surgeon, making the correct decisions at the end of the procedure, which had not been previously examined.MethodsFrom January 2017 to March 2018, 364 consecutive patients requiring PCNL were comprehensively analyzed preoperatively and patients were selected for scheduled tubeless PCNL based on four aspects. The selected patients were divided into two groups according to whether the nephrostomy tube was finally placed. The mean operative time, intraoperative blood loss, stone clearance rate, visual pain score, postoperative hospitalization days and perioperative complications were all evaluated.ResultsBased on the preoperative evaluation, 42 patients were selected for tubeless PCNL, among which there were finally 37 cases of completed tubeless PCNL. Compared with patients undergoing conventional PCNL, there were not statistical differences in the mean operative time (P=0.207) or intraoperative blood loss (P=0.450) in the tubeless group. Stone clearance rate was 100% in both groups. The visual pain scores in the tubeless PCNL group were lower on operation day (P=0.029), first postoperative day (P<0.001) and the day of discharge (P=0.025). The postoperative hospitalization for the tubeless PCNL group was shorter than that of the control group (P<0.001). No significant difference in grade 1 complications was seen (P=0.424), and no grade 2 or higher complications were observed in either group.ConclusionsPostoperative pain was significantly relieved and postoperative hospitalization was significantly shortened in the tubeless PCNL group. Tubeless PCNL is safe if patients are carefully selected using four criteria before operation, attention is paid to four key points and five confirmations are made during operation.