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Hjelmevoll SO Olsen ME Sollid JU Haaheim H Melby KK Moi H Unemo M Skogen V 《Sexually transmitted diseases》2008,35(5):517-520
BACKGROUND: Diagnosing Neisseria gonorrheae using nucleic acid amplification tests (NAATs) might increase the sensitivity, compared to cultivation. However, using NAATs has also been problematic mainly due to the close genetic relationships between different Neisseria species, resulting in false positive diagnoses. This study was conducted to clinically validate a previously published real-time polymerase chain reaction (PCR) method targeting the porA pseudogene in N. gonorrheae in comparison to culture techniques. METHODS: In total, 360 samples, urethra (n = 109), rectum (n = 84), pharynx (n = 119), and cervix (n = 48) from 185 males and 57 females, were analyzed using porA pseudogene PCR and cultivation. Sequencing of the entire porA pseudogene and the 16S rRNA gene were used to resolve discrepant results. RESULTS: Of the 360 samples, 37 were positive by both culture and PCR, however, the PCR identified 15 additional confirmed positive samples. The PCR method showed a sensitivity, specificity, positive predictive value, and negative predictive value of 100% in a preselected population. The preselected population had a true gonorrhea prevalence of 17.4%. CONCLUSIONS: The present porA pseudogene real-time PCR comprises a valuable supplement to the traditional culture techniques for diagnosis of N. gonorrheae, especially for samples from extragenital sites such as pharynx and rectum. 相似文献
73.
George Rachiotis Varvara A Mouchtouri Clara Schlaich Tobias Riemer Carmen Varela Martinez Gordon Nichols Christopher LR Bartlett Jenny Kremastinou Christos Hadjichristodoulou the SHIPSAN partnership 《Journal of occupational medicine and toxicology (London, England)》2010,5(1):1
Background
Seafarers play an important role in the transmission of communicable diseases. The aim of the present study is to draw information and identify possible gaps on occupational health practices related to seafarers sailing on ships within the European Union Member States (EU MS) with focus on communicable diseases. 相似文献74.
1背景与资料上世纪80和90年代,美国的医院和医生组织投入大量时间和资源进行机构重组,以实现提高组织效率、改善财务绩效水平、机构长期生存、承担社区责任和救治病人的目的。而机构重组的主要趋势是建立“有组织的服务提供体系”,包括建立横向的医院联合体,横向的医生联合体,以及纵向的医院与医生联合体等。到目前为止,在美国开展的大量研究对上述机构重组效果的认识莫衷一是。 相似文献
75.
目的测定甘肃产五加中剌五加苷B、苷E的含量。方法高效液相色谱法,ODSKromasal柱。水乙晴(955)为流动相,检测波长222nm,柱温度25℃。结果本文可同时测定剌五加苷B、苷E的含量。剌五加苷B、苷E分别在0.064~0.320μg/ml;0.074~0.370μg/ml范围内峰面积与浓度呈线性关系,平均回收率分别为102.5%,RSD=4.2%,95.5%,RSD=4.6%。结论剌五加苷B、苷E在红毛五加中含量最高;茎皮中含量最高;剌五加苷E的含量高于苷B。 相似文献
76.
The right mid-lung window 总被引:1,自引:0,他引:1
Goodman LR; Golkow RS; Steiner RM; Teplick SK; Haskin ME; Himmelstein E; Teplick JG 《Radiology》1982,143(1):135
77.
Ahn JM; Kwak SM; Kang HS; Muhle C; Pedowitz RA; Frank LR; Trudell D; Haghighi P; Resnick D 《Radiology》1998,208(1):57
78.
79.
Complications of automatic implantable cardioverter defibrillators: radiographic, CT, and echocardiographic evaluation 总被引:1,自引:0,他引:1
Goodman LR; Almassi GH; Troup PJ; Gurney JW; Veseth-Rogers J; Chapman PD; Wetherbee JN 《Radiology》1989,170(2):447-452
Automatic implantable cardioverter defibrillators (AICDs) were studied in three groups: (a) Serial radiographs were reviewed in 51 clinic patients. Twenty of 96 (21%) AICD patches distorted with time. (b) Thirty-six postoperative computed tomographic (CT) scans of asymptomatic patients revealed that pericardial fluid collections were frequent during the month after surgery but rare beyond that. Echocardiography was insensitive for these collections. CT also demonstrated dense fibrosis around some distorted patches, months after surgery. (c) Five other patients with pericardial infection had distorted patches, and the four studied with CT had fluid beneath their patches. (d) A case of constrictive pericarditis had distorted patches but was not diagnosed with CT. The authors conclude that distorted patches may indicate postoperative complications and that CT is the imaging modality of choice. 相似文献
80.