Efficiency of sex pre-selection of spermatozoa by albumin separation method evaluated by double-labelled fluorescence in-situ hybridization

To evaluate the separation efficiency of Ericsson's two- and three- layer albumin separation methods, semen samples from 21 healthy males were studied. Seven patients already had two or more sons, another seven had two or more daughters and the other seven had primary infertility due to female factors. The semen samples were divided into three aliquots: one remained unprocessed initially, the other two aliquots went through two- and three-layer albumin separation methods respectively. All samples were then stained with X-Y double staining probes. In each group, four or five samples were processed at room temperature, and two or three at body temperature (37 degrees C). The labelling efficiency of X-Y double staining probe was over 99%. The X:Y sperm ratios were even in the original samples. The ratios of the X and Y spermatozoa were altered slightly but significantly after the two- layer (P < or = 0.05) or the three-layer (P < or = 0.005) separation. The alterations occurred only at room temperature. The X spermatozoa increased and the Y spermatozoa decreased, both to a small degree of difference (1.4-3.5%). Double fluorescence in-situ hybridization analysis therefore showed that albumin separation methods do not enrich Y spermatozoa.      

Ethmoid sinus disease: CT evaluation in 400 cases. Part II. Postoperative findings

Clinical, pathologic, and computed tomographic (CT) findings were reviewed in 400 patients with ethmoid sinus disease, including 272 who had undergone surgery. The postoperative appearance of ethmoid sinuses on CT scans is a highly neglected subject in radiologic literature. The unique ethmoid anatomy permits a variety of surgical approaches, and radiologists must be familiar with the postoperative appearances if they are to recognize the effects of prior surgery and detect, when possible, the recurrence of disease. It is often difficult to evaluate the clinical significance of soft-tissue disease, despite good clinical correlation.     

The emerging dental workforce: why dentistry? A quantitative study of final year dental students' views on their professional career

Background  

Dental graduates are joining a profession experiencing changes in systems of care, funding and skill mix. Research into the motivation and expectations of the emerging workforce is vital to inform professional and policy decisions. The objective of this research was to investigate final year dental students' perceived motivation for their choice of career in relation to sex, ethnicity and mode of entry.     

血清HBV-DNA水平的变化与慢性乙型活动性肝炎的关系

目的:研究慢性肝炎患者HBV-DNA水平与病情变化的关系. 方法:采用荧光标记定量PCR的方法,测定慢性乙型肝炎急性发作患者(HBeAg阳性45例,抗HBe阳性30例)其活动期和恢复期的血清HBV-DNA含量. 结果:HBeAg阳性组血清HBV-DNA含量(Logarrithm copy/mL)在活动期显著高于恢复期[(10.2±1.6) vs (5.3±2.1), P<0.05],抗-HBe阳性组血清HBV-DNA含量(Logarrithm copy/mL)在活动期显著高于恢复期[(7.6±1.3) vs (4.0±2.0), P<0.05]. 另外,活动期血清HBV-DNA含量(Logarrithm copy/mL)在HBeAg阳性组显著高于抗HBe阳性组[(10.2±1.6) vs (7.6±1.3), P<0.05]. 血清HBV-DNA水平与丙氨酸转氨酶(ALT)、门冬氨酸转氨酶(AST)及总胆红素(T-BLLI)之间无相关性. 结论:HBV-DNA复制水平与慢性乙型病毒性肝炎的活动密切相关.     

德国SIAB-S量表在国内应用的评量标准

目的:分析SIAB-S的评量标准在国内(湖北省宜昌地区)的适用性以及量表各因子间同质性信度分析。方法:采用随机抽样和整群抽样结合方法抽取2004/2005宜昌第14中学、宜昌卫生学校509名学生为调查对象。采用德国SIAB-S量表对抽样对象进行自评筛查和以访谈方式采用DSM-Ⅳ标准进行临床诊断。其中德国SIAB-S量表(1998年第3版),含有87个问题,经归类后主要包括6个方面(因子)的内容:①普通心理病理学和社会整合能力。②体形和苗条理想。③贪食症状。④性欲和体重。⑤相应控制方法、节食和物质滥用。⑥非典型进食发作。询问在最近3个月(目前状态)和最近3个月前(从前)明显形成的症状。对两种方法得出的阳性结果进行对照分析。并使用SSPS11.5统计分析软件对SIAB-S量表进行同质性信度分析。结果:发放问卷509份,收回有效问卷451份,男生75名,女生376名,年龄15～19岁。①德国SIAB-S量表标准:过去发生进食障碍12名;目前存在进食障碍6名;进食障碍总计13名;发病率2.88%。DSM-Ⅳ标准:过去发生进食障碍13名;目前存在进食障碍8名;进食障碍总计16名;发病率3.55%。其中有10名是使用两种方法均能检查出。进行χ2检验,χ2=0.444,P>0.05。差异无显著性。②针对目前诊断进食障碍,该量表的重复度量效果良好(F=897.02,P<0.01)。其6项因子间的标准化信度为0.49,说明该量表的信度还有待进一步的开发完善。对于以前诊断进食障碍,该量表的重复度量效果同样良好(F=547.29,P<0.01)。其6项因子间的标准化信度为0.62,说明该量表的信度较好。结论:SIAB-S量表的德国常模在国内适用,有待进一步完善。     

Effectiveness of prolonged use of continuous passive motion (CPM), as an adjunct to physiotherapy,after total knee arthroplasty

Background  

Adequate and intensive rehabilitation is an important requirement for successful total knee arthroplasty.