环境内分泌干扰物的安全性评价研究进展

环境激素或内分泌干扰物因为其具有类似在生物体内的激素功能，能抑制其作用，进而改变生物体内生殖、免疫、神经内分泌等系统的正常运作。本文对这类化学物质对人和动物的影响和安全性评价研究的进展进行了初步的阐述。     

大鼠注射重组腺病毒Ad-HGF后血清中抗腺病毒抗体的检测

目的观察大鼠重复给与携带肝细胞生长因子基因的重组腺病毒（recombinant adenovirus carrying hepatocyte growth factor gene，Ad-HGF）后，血清中抗腺病毒抗体的出现时间及消失规律。方法大鼠给与不同剂量的Ad-HGF共14次，利用病毒中和反应（细胞病变效应及绿色荧光方法）观察血清中抗腺病毒抗体。结果手术及正常对照组和实验组动物给药7次前血清处理的细胞均出现明显细胞病变效应及强荧光。之后各剂量组动物血清均对腺病毒有不同程度的中和作用，停药后13周到14周抗体水平很低或已不存在。同时观察到动物性别及给药剂量大小无差异。结论大鼠肌注Ad-HGF7次后，动物血清中可检测到抗腺病毒抗体，停药后13周或14周抗体基本消失，此结果对Ad-HGF的实际应用具有指导意义。     

民办高校大学新生SCL—90量表测评分析

目的：了解民办高校大学一年级新生心理健康状况。方法：采用临床症状自评量表(SCL—90)，对中国信息大学一年级全体新生进行心理测量，并对1132个有效样本进行了比较分析。结果：①被试SCL—90测查中度或中度以上的心理问题检出率16．70％，男生检出率为16．15%，女生检出率为17．62％，男女生无显性差异；②不同群体比较：干部、知识分子家庭子女、高收入家庭及低收入家庭子女心理问题发生率较高；③按各种标准分类，不同群体被试的排在前几位的心理问题几乎均为人际关系敏感、强迫症状和抑郁，敌对症状也较为突出。结论：民办高校大学新生心理问题检出率较高，应予关注。     

银杏叶片改善高原移居者脑一体功效能力的对比研究

目的 探讨药物提高和改善高原移居者脑-体作业能力的作用。方法选择驻海拔3700m10个月的某部士兵10人,于服药前、负荷运动前后、服药15d负荷运动前后4个不同阶段采用DDX200型电脑心理生理测试仪进行缺失记忆,左右手及左右脚交叉动作频率的测验,进行服药前后与负荷运动前后的总次数、正确与错误次数的比较。结果服用银杏叶片15d,运动前后缺失记忆在相同时间操作时限内(10s),错误次数(7.0±2.3,8.0±2.8)与服药前运动前后相同时段比较错误次数(11.0±2.7,16.0±2.1),显著减少(P<0.01或P<0.05)。服用银杏叶片15d,运动前后在相同的操作时限内(10s)左右手交叉敲击动作频率正确次数54.0±7.0,52.0±5.1。错误次数11.0±5.3,11.0±5.6与服药前运动前后比较正确次数23.0±6.4,20.0±0.9,错误次数18.0±4.1,19.0±5.4比较,正确次数显著增多(P<0.01),错误次数显著减少(P<0.01)。服用银杏叶片15d,运动前后在相同的操作时限内(10s),左右脚交叉动作频率正确次数为57.0±5.7,50.0±6.9,错误次数7.0±2.4,8.0±3.1与服药前运动前后正确次数34.0±6.8,29.0±5.4,错误次数13.0±4.3,15.0±4.5比较相差非显著(P<0.01或P<0.05)。结论口服银杏叶片15d能显著提高和改善高原移居者脑-体作业效率和能力。     

Effect of Modified Sinisan (四逆散) on Anorectal Manometry of the Constipation Predominant Type of Irritable Bowel Syndrome

Irritable bowel syndrome (IBS) of theconstipation predominant type is a functionalintestinal disease characterized by abdominaldistress or pain, changed habit of defecationand abnormal defecation. Its pathogenesishas not been totally clarified yet. This re search is to explore the mechanism of pa tients with IBS of the constipation predomi nant type through the determination of ano rectal pressure via anus and better efficacyhas been obtained in the study by modify…     

年轻妇女宫颈癌临床特征分析

目的分析35岁以下年轻妇女宫颈癌的发病趋势、临床特征变化等.方法对1998年-2003年我院收治的67例年轻宫颈癌患者（研究组）的临床资料进行回顾性分析,并随机选择同期有完整随访资料的82例年龄大于35岁的宫颈癌患者作对照组,分析两组资料间临床特点、病理及预后之间的差异.结果 1998年-2003年6年间年轻宫颈癌的构成比呈现逐渐上升趋势,分别为11.11%、13.63%、15.21%、17.28%、20.89%、23.58%;研究组以接触性出血为主要临床表现,占79.10%（53/67）,与对照组的40.24%（33/82）比较有显著性差异（P＜0.01）;初诊时67例患者中30例（44.77%）曾在基层医院以宫颈炎、宫颈糜烂行对症治疗,12例（17.91%）以月经紊乱行调经治疗,1例术前诊断为子宫肌瘤,术后病检证实为宫颈癌;病理特点研究组内生型比率较对照组明显增高,二者比较有显著性差异;病理类型及组织类型上二者无明显差异,均以鳞癌为主（97.01%、95.21%）,但术后淋巴病理检查显示对照组淋巴转移率高于研究组,有显著性差异.结论年轻宫颈癌患者的构成比呈现明显上升趋势,宫颈癌年轻化趋势明显;年轻宫颈癌临床症状较为隐匿,容易漏诊和误诊.     

成人脂肪间充质干细胞体外定向诱导分化为心肌样细胞的实验观察

目的:探索成人脂肪组织来源的间充质干细胞(ADMSCs)体外分离、培养方法,并化学诱导使其向心肌细胞分化,为心肌再生医学提供理想的种子细胞来源. 方法:体外分离成人ADMSCs并进行传代培养,5-氮胞苷(5-aza)诱导其向心肌细胞分化. 分别于诱导后14,21,28 d时用免疫细胞化学染色、RT-PCR法进行心肌细胞鉴定. 结果:ADMSCs经5-aza诱导后14 d时细胞形态趋向于心肌细胞,诱导28 d免疫细胞化学显示心肌特异性肌钙蛋白I(cTN-I)、结蛋白(Desmin)表达阳性,RT-PCR检测心肌β-肌球蛋白重链(Cardiac β-MHC)基因表达阳性. 结论:成人脂肪组织存在丰富的间充质干细胞且易于分离扩增,体外5-aza诱导可使其向心肌细胞分化,可做为心肌再生医学的理想种子细胞.     

中药灯盏细辛中酚酸类化合物的结构与活性研究

目的 研究中药灯盏细辛的化学成分。方法 利用硅胶柱层析等分离手段从灯盏细辛[Erigeron breviscapus(Vant.)Hand-Mazz]的醋酸乙酯部位分离得到2个化合物，采用波谱方法（IR，MS，^1H-NMR,^13C-NMR,2D-NMR)鉴定了它们的结构。结果 2个酚酸类化合物分别为：5－O－咖啡酰基－奎宁酸甲酯（I）和4－O－咖啡酰基－奎宁酸甲酯（Ⅱ），结论 化合物Ⅰ，Ⅱ为首次从灯盏细辛中分得的酚酸类化合物。药理活性研究显示，化合物Ⅰ，Ⅱ可明显抑制VEGF诱导的血管高通透性（P＜0．001）。     

The incidence of prostate cancer in a screening population with a serum prostate specific antigen between 2.5 and 4.0 ng/ml: relation to biopsy strategy

PURPOSE: It has recently been suggested that the diagnostic threshold for the prostate specific antigen (PSA) assay be lowered to enhance prostate cancer detection. A 22% incidence of prostate cancer has been reported in men with PSA between 2.5 and 4.0 ng/ml. We designed a study to confirm this observation. MATERIALS AND METHODS: Men who participated in our free early detection program and who had serum PSA between 2.5 and 4.0 ng/ml were asked to undergo prostate biopsy. Of 268 eligible men 151 (56%) agreed to participate in this free trial. All men underwent biopsy using an 11-core multisite directed biopsy scheme. All biopsy cores were color coded for location specificity and examined by 1 pathologist. RESULTS: Cancer was identified in 24.5% (37 of 151) of the men biopsied. The median age of men with cancer was 62 years (range 43 to 74). Conventional systematic sextant biopsies, which accounted for 6 of the 11 cores, detected 73.0% (27 of 37) of the cancers and the alternate site biopsies identified the remaining 10. Gleason score was 6 in 25 men, 3 + 4 in 5, 4 + 3 in 4 and 8 or greater in 3 (median Gleason score 6). There were 14 men who had 1 core positive for cancer, 9 had 2 and 14 had more than 2 (median number of positive cores 2). Of the 14 men with 1 positive core 11 had a less than 3 mm focus of cancer and 8 had only a positive alternate site biopsy. There were 11 cases of abnormal results on digital rectal examination, 5 of which were cancer, and 31 cases of abnormal results on ultrasonography, 13 of which were cancer. Median biological variability in PSA was +/-15% (range 0.4% to 440.0%). CONCLUSIONS: We found a significant incidence of cancer (24.5%, 37 of 51) in men with serum PSA between 2.5 and 4.0 ng/ml. In our study 67.6% of the detected cancers were significant based on the biopsy data. If the PSA threshold is lowered the conventional systematic sextant technique may be preferable to an extended strategy.     

携带HGF基因的减毒沙门氏菌对大鼠胃溃疡愈合及CD34表达的影响

目的:通过对乙酸性胃溃疡大鼠口服携带肝细胞生长因子(HGF)基因的减毒鼠伤寒沙门氏菌(St)后检测胃溃疡愈合情况及局部和CD34蛋白表达的研究,探讨基因药物St-HGF对乙酸性大鼠胃溃疡的治疗作用.方法:建立乙酸性胃溃疡大鼠77只,并随机选出5只,给予携带绿色荧光蛋白(GFP)基因的st后观察GFP在大鼠胃壁中的分布,剩余大鼠随机分为3组(n=24):St-HGF治疗组,St治疗组,NaHCO3对照组;增菌后各组动物于造模第2日起灌胃相应药物,隔日一次,分别于给药后7,14,21 d取胃溃疡组织行HE病理组织学观察,免疫组化法检测CD34蛋白的表达.结果:给药后7 d光镜下溃疡部位黏膜消失,局部有大量坏死组织,各组胃黏膜上皮爬行不明显;给药后14,21 d,St-HGF组黏膜上皮增生较另两组快(P<0.01);给药后7,14,21 d,CD34蛋白在St-HGF组的表达高于另两组(P<0.05),组间有统计学差异.结论:St-HGF可促进实验性大鼠胃溃疡黏膜上皮修复和溃疡面血管增生,加快溃疡愈合.