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31.
32.
Claudia L. Sánchez-Camargo Pedro Albajar-Vi?as Patricia P. Wilkins Javier Nieto David A. Leiby Luc Paris Karenina Scollo Carolina Flórez Carmen Guzmán-Bracho Alejandro O. Luquetti Nidia Calvo Kenji Tadokoro Amadeo Saez-Alquezar Pedro Pablo Palma Miguel Martin Laurence Flevaud 《Journal of clinical microbiology》2014,52(7):2506-2512
Chagas disease is one of the main public health issues in Latin America. Increasingly during the past few decades, Trypanosoma cruzi infection has been detected in North America, Europe, and the Western Pacific, mainly as a result of population movement. The limited availability of rapid serological diagnostic tests hinders rapid diagnosis and early treatment in areas of endemicity and nonendemicity. In collaboration with 11 national reference laboratories (NRLs) from different geographical areas, we evaluated the performances of commercialized serological rapid diagnostic tests (RDT) for T. cruzi infection. Eleven commercialized T. cruzi infection RDTs were evaluated on a total of 474 samples extensively tested with at least three different techniques for Chagas disease, maintained at controlled low temperatures, and stored in the serum banks of the 11 NRLs. We measured the sensitivity, specificity, and concordance of each RDT and provided an additional questionnaire to evaluate its ease of use. The selected RDTs in this study were performed under controlled laboratory conditions. Out of the 11 RDTs, we found 8 of them to be useful, with the cassette format favored over the strip. We did not observe significant differences in RDT performances in the different regions. Overall, the performance results were lower than those disclosed by the manufacturers. The results of this evaluation validate the possibility of using RDTs to diagnose Chagas disease, thereby decreasing the time to treatment at a primary health care facility for patients who are willing to be treated. Further studies should be conducted in the laboratory and in the field to confirm these data, expressly to evaluate reproducibility in resource-limited settings, or using whole blood in clinical settings in areas of endemicity and nonendemicity. 相似文献
33.
B Sierra AB Pérez M Alvarez G García K Vogt E Aguirre K Schmolke HD Volk MG Guzmán 《The American journal of tropical medicine and hygiene》2012,87(3):538-547
Abstract. Secondary heterologous dengue infection is a risk factor for severe disease manifestations because of the immune-enhancement phenomenon. Succeeding clinical infections are seldom reported, and the clinical course of tertiary and quaternary dengue infections is not clear. Cuba represents a unique environment to study tertiary/quaternary dengue infections in a population with known clinical and serologic dengue markers and no dengue endemicity. We took advantage of this exceptional epidemiologic condition to study the effect of primary, secondary, tertiary, and quaternary dengue infection exposure on the expression of pro-inflammatory and regulatory cytokines, critical in dengue infection pathogenesis, by using a dengue infection ex vivo model. Whereas secondary exposure induced a high cytokine response, we found a significantly lower expression of tumor necrosis factor-α, interferon-γ, interleukin-10, and tumor growth factor-β after tertiary and quaternary infectious challenge. Significant differences in expression of the cytokines were seen between the dengue immune profiles, suggesting that the sequence in which the immune system encounters serotypes may be important in determining the nature of the immune response to subsequent infections. 相似文献
34.
Francisco Javier Guzmán-de la Garza Juan Manuel Ibarra-Hernández Paula Cordero-Pérez Pablo Villegas-Quintero Claudia Ivette Villarreal-Ovalle Liliana Torres-González Norma Edith Oliva-Sosa Gabriela Alarcón-Galván Nancy Esthela Fernández-Garza Linda Elsa Mu?oz-Espinosa Carlos Rodrigo Cámara-Lemarroy José Gerardo Carrillo-Arriaga 《Clinics (S?o Paulo, Brazil)》2013,68(7):1034-1038
OBJECTIVE:
It is essential to identify a serological marker of injury in order to study the pathophysiology of intestinal ischemia reperfusion. In this work, we studied the evolution of several serological markers after intestinal ischemia reperfusion injury in rats. The markers of non-specific cell damage were aspartate aminotransferase, alanine aminotransaminase, and lactic dehydrogenase, the markers of inflammation were tumor necrosis factor alpha, interleukin-6, and interleukin-1 beta, and the markers of intestinal mucosal damage were intestinal fatty acid binding protein and D-lactate. We used Chiús classification to grade the histopathological damage.METHODS:
We studied 35 Wistar rats divided into groups according to reperfusion time. The superior mesenteric artery was clamped for 30 minutes, and blood and biopsies were collected at 1, 3, 6, 12, 24, and 48 hours after reperfusion. We plotted the mean ± standard deviation and compared the baseline and maximum values for each marker using Student''s t-test.RESULTS:
The maximum values of interleukin-1 beta and lactic dehydrogenase were present before the maximal histopathological damage. The maximum tumor necrosis factor alpha and D-lactate expressions coincided with histopathological damage. Alanine aminotransaminase and aspartate aminotransferase had a maximum expression level that increased following the histopathological damage. The maximum expressions of interluken-6 and intestinal fatty acid binding protein were not significantly different from the Sham treated group.CONCLUSION:
For the evaluation of injury secondary to acute intestinal ischemia reperfusion with a 30 minute ischemia period, we recommend performing histopathological grading, quantification of D-lactate, which is synthesized by intestinal bacteria and is considered an indicator of mucosal injury, and quantification of tumor necrosis factor alpha as indicators of acute inflammation three hours after reperfusion. 相似文献35.
Guadalupe del Carmen Baeza-Flores Erick J. Rodríguez-Palma Valeria Reyes-Pérez Crystell G. Guzmán-Priego Jorge E. Torres-López 《Drug development research》2020,81(6):728-735
Ceftriaxone (CFX) is a β-lactam antibiotic with analgesic properties. However, its role in the formalin-induced nociception remains unknown. The purpose of this study was to investigate the antinociceptive effect of CFX in the 1% formalin test in rats. Formalin induced a typical nociceptive response (flinching behavior) of two phases. Local peripheral pretreatment (20 min) with CFX (400–800 μg/paw) slightly attenuated the flinching behavior in phase 2, but not phase 1. Acute intraperitoneal pretreatment (20 min) also reduced phase 2 of the formalin test. In both cases, CFX induced a dose-dependent antinociception. We also tested the effect of CFX 1 day after its administration and in two schedules of repeated administration. One-day pretreatment with CFX (50–400 mg/kg, ip) induced a dose-dependent antinociceptive effect in formalin-treated rats. Repeated administration (daily during 3 or 7 days) with CFX (50–400 mg/kg, ip) diminished formalin-induced nociception. Results suggest that local or systemic as well as single or repeated administration of CFX reduces formalin-induced nociception. 相似文献
36.
García-Reyna Benjamín Castillo-García Gilberto Daniel Barbosa-Camacho Francisco José Cervantes-Cardona Guillermo Alonso Cervantes-Pérez Enrique Torres-Mendoza Blanca Miriam Fuentes-Orozco Clotilde Pintor-Belmontes Kevin Josue Guzmán-Ramírez Bertha Georgina Bernal-Hernández Aldo González-Ojeda Alejandro Cervantes-Guevara Gabino 《International journal of mental health and addiction》2022,20(2):895-906
International Journal of Mental Health and Addiction - The presence of COVID-19 has had psychological consequences among health personnel; these include fear, anxiety, and depression. In the... 相似文献
37.
Hans Peter Dietz Alejandro Pattillo Garnham Rodrigo Guzmán Rojas 《International urogynecology journal》2016,27(6):909-913
Introduction and hypothesis
Levator avulsion is a risk factor for female pelvic organ prolapse (POP) and recurrence after POP surgery. Imaging diagnosis requires the observation of an abnormal muscle insertion on tomographic ultrasound imaging (TUI). This study was designed to compare the diagnostic performance of the qualitative diagnosis (visual qualitative assessment) to measurement of the distance between muscle insertion and urethra [levator–urethra gap; (LUG)].Methods
This was a retrospective analysis of data obtained in a tertiary urogynecological unit. All patients presented with symptoms of pelvic floor dysfunction and underwent 4D translabial pelvic floor ultrasound (US), supine, and after voiding. Avulsion was defined qualitatively as abnormal muscle insertion and quantitatively as LUG ≥25 mm on at least three consecutive central axial plane slices, with one examiner using both methods. We examined the correlation between both methods and validated them against clinical prolapse, significant organ descent on US, and hiatal ballooning.Results
Between January and July 2013, 233 patients were seen, of whom 202 had complete volume data sets. The qualitative method diagnosed avulsion in 22 % and the quantitative method in 24.3 %. Agreement was good, with a kappa of 0.79 (0.70–0.87). Avulsion diagnosed by either method was associated with clinical and sonographic prolapse and hiatal ballooning, with odds ratios nonsignificantly higher for the quantitative method.Conclusion
Qualitative analysis of slices on TUI and a method using LUG measurement show good agreement for the diagnosis of avulsion. The LUG method is at least equally as valid in its capacity to predict significant prolapse on clinical examination and US, as well as ballooning of the levator hiatus.38.
Jaime Alfaro Javier Guzmán Catalina García David Sirlopú Fernando Reyes Jorge Varela 《Child indicators research》2016,9(3):731-742
Child and adolescent measures of the quality of life or subjective well-being are scarce in Latin American countries. Chile is no exception to this situation that represents a gap in the literature for South American countries research. Thus, the aim was to analyze the psychometric properties of the Personal Well-being Index for School Children (PWI-SC) of Cummins et al. Social Indicators Research, 64(2), 159–190, (2003) in a sample of Chilean school children. We use a sample of 1096 pupils between 10 and 12 years old (M?=?11.03; SD?=?0.89) and residents of three major Chilean cities. Results of Exploratory and Confirmatory Factor Analysis provided evidence for only one single factor solution. Moreover, an acceptable level of internal reliability was observed (α?=?0.77). The discussion of results considers other internationals applications of PWI-SC. 相似文献
39.
Rodolfo Morales-Avalos Arlette Gabriela Santos-Martínez Cesia Gisela Ávalos-Fernández Karim Mohamed-Noriega Gabriela Sánchez-Mejorada Adolfo Montemayor-Alatorre David A. Martínez-Fernández Abraham G. Espinosa-Uribe Jibran Mohamed-Noriega Edgar E. Cuervo-Lozano Jesús Mohamed-Hamsho Oscar Quiroga-García Roberto A. Lugo-Guillen Santos Guzmán-López Rodrigo E. Elizondo-Omaña 《European archives of oto-rhino-laryngology》2016,273(9):2785-2793
40.
Natalia Moreno-Castellanos Rocío Guzmán-Ruiz David A. Cano Ainara Madrazo-Atutxa Juan R. Peinado Jose L. Pereira-Cunill Pedro Pablo García-Luna Salvador Morales-Conde Maria Socas-Macias Rafael Vázquez-Martínez Alfonso Leal-Cerro María M. Malagón 《Obesity surgery》2016,26(8):1757-1767