构建聚丙交酯-乙交酯共聚物包埋甲壳胺无纺布的新型高聚物材料支架

目的:利用聚丙交酯-乙交酯共聚物强度大和甲壳胺无纺布可塑性好的特点,制作出一种力学性能良好及空隙均匀的新型聚合物支架。方法:实验于2004-05/12在第一军医大学珠江医院全军儿科中心实验室和中山大学高分子研究所进行。将2倍甲壳胺无纺布质量的聚丙交酯-乙交酯共聚物溶解于氯仿中,聚合物浓度为20g/L,将甲壳胺无纺布反复浸泡其中、烘干,直至均匀交联。去除氯仿细胞毒后,所制作的支架,测定其力学性能及孔隙率。结果:①新型聚合物支架抗压缩强度为1.4～2.0MPa,弯曲强度达16.3MPa,剪切强度为48MPa。②新型聚合物支架孔隙率达到82%～86%,孔径在100～300μm之间。结论:使用此法制作的组织工程支架,强度和可塑性较好,三维结构稳定,各项参数可控制,可以制作较大型体积的支架。     

氯胺酮在吗啡急性耐受大鼠的外周镇痛作用

我们产证明氯胺酮引起的外周镇痛作用与阿片受体的激活有关。本实验旨在建立吗啡外周镇痛耐受的大鼠模型，进一步观察氯胺酮的外周镇痛作用与阿片受体的关系。外周感受野局部皮下注射５μｌ吗啡（１０μｇ／μｌ）明显的抑制伤害性肌电反应。随吗啡注射次数的增加，伤害性反应的抑制逐渐减弱，一般于第五次注射，吗啡不再产生抑制，出现外周镇痛的急性耐受。但是，在耐受动物的同一部位注射０μｌ氯胺酮（５０μｇ／μｌ），仍产生很     

圈套器联合棉线牵引辅助在内镜黏膜下剥离上消化道早癌中的应用研究

目的研究新型辅助技术圈套器联合棉线在内镜黏膜下剥离上消化道早癌病变中的应用研究。方法回顾性研究2016年6月-2019年2月在该院内镜中心接受治疗的上消化道早癌患者40例,分为对照组和改良牵引组,每组各20例。对照组采用传统的内镜黏膜下剥离术(ESD),改良牵引组使用圈套器联合棉线牵引辅助行ESD,分别从手术完整切除率、手术时间、术中及术后并发症、术后平均住院天数等方面进行对比。结果术前两组患者在年龄、性别构成、肿瘤部位及直径等方面差异均无统计学意义(P>0.05)。两组上消化道早癌患者完整切除率为100%。对照组手术时间为(73.95±13.04)min,改良牵引组为(50.95±12.62)min,改良牵引组手术时间明显短于对照组,差异有统计学意义(t=4.74,P<0.01)。对照组有2例发生术中出血、2例术后发生迟发性出血及穿孔,均通过内镜下处理好转出院。对照组住院时间为(10.21±3.74)d,改良牵引组为(6.05±2.56)d,改良牵引组住院时间明显短于对照组,差异有统计学意义(t=9.15,P<0.01)。所有标本术后病理底切缘及侧切缘均为阴性。40例患者术后随访至截稿时均未复发,无术后食管胃狭窄等远期并发症。结论圈套器联合棉线牵引辅助装置能给ESD操作者提供良好的手术视野,在缩短手术时间的同时,降低并发症的发生风险,而且该装置组合简便,能作为ESD治疗黏膜病变过程中的一个有效辅助装置。     

长期经口铝摄入对大鼠记忆行为的影响

目的:观察长期经口摄入铝对大鼠记忆行为的影响及吡拉西坦(商品名:脑复康)的预防作用。方法:Wistar大鼠按体质量随机分为3组,每组10只。Ⅰ组为对照组。每天自由进食及饮水,同时以2mL生理盐水灌胃;Ⅱ组自由进食但饮水中含硫酸铝25g/L(含铝2g/L),同时以2mL生理盐水灌胃。Ⅲ组进食及饮水同Ⅱ组,但每天以50mg吡拉西坦溶于2mL生理盐水中灌胃。3个月后停止生理盐水及吡拉西坦灌胃,继续喂养,应用被动回避实验,观察各组大鼠记忆消退的时间。结果:单纯用硫酸铝大鼠试验后第3周出现记忆消退,3,4,5,6周记忆消失百分数分别为20%,30%,80%,100%;应用吡拉西坦大鼠第3周出现记忆消退,3,4,5,6,7,8,9周记忆消失百分数分别为10%,10%,10%,10%,20%,70%,100%;对照组第7周出现记忆消失,第8周记忆消失百分数达90%,第9周达100%。结论:长期经口摄入铝对大鼠记忆行为有损害作用,吡拉西坦可预防其行为的损害。     

精准护理在主动脉夹层动脉瘤患者围手术期中的应用效果

目的探讨精准护理应用在主动脉夹层动脉瘤患者围手术期中的效果。方法选取2014年6月—2017年6月因主动脉夹层动脉瘤于首都医科大学附属北京安贞医院进行手术治疗的57例患者,随机分为观察组(n=29)和对照组(n=28),对照组采用常规护理模式,观察组采用主动脉疾病精准护理模式。比较两组患者血压变化情况、并发症发生率。结果术后观察组患者收缩压(122.5±12.8)mmHg,舒张压(69.3±7.2)mmHg,均低于对照组,差异有统计学意义(P<0.05)。观察组患者发生术后综合征2例,对照组8例,差异有统计学意义(P<0.05)。结论精准护理模式可有效控制主动脉夹层动脉瘤患者术后血压,降低并发症发生率,有临床推广应用价值。     

综合护理干预对老年管饲患者焦虑和抑郁的影响

目的探讨综合护理干预对老年管饲患者焦虑和抑郁情况的影响。方法将研究对象93例按就诊顺序分为观察组和对照组。对照组实施常规护理，观察组除常规护理外，进行综合护理于预。两组在干预前、后均予汉密顿焦虑量表和汉密顿抑郁量表进行评估，比较两组患者焦虑和抑郁的情况。结果干预2周后，两组患者焦虑、抑郁评分差异都有统计学意义（P〈0．01）。结论综合护理干预能明显减轻老年管饲患者的焦虑、抑郁症状，提高患者的生活质量。     

多发性硬化298个造影增强病灶特点

目的　了解多发性硬化 (MS)增强磁共振成像 (MRI)病灶的特点。方法　选择确诊MS186例 ,检查增强头颅MRI,发现阳性病灶后 ,每 (4± 1)周 1次头颅钆喷替酸葡甲胺造影剂 (GD)增强MRI ,观察了 2 98个病灶的形态及变化情况。结果　造影病灶形态可为圆形、卵圆形、环形、弧形、点状和不规则形。 2 98个GD增强病灶中 ,2 0 7个 (6 9 5 % )在发现后的 4周内消失 ,2 5 1个 (84 2 % )于 8周内消失 ,2 72个 (91 3% )于 12周内消失 ,3个病灶持续存在 2年以上。结论　MSGD增强MRI病灶绝大多数在 4周内消失 ,少数可持续 2年以上 ;为了观察MS脑内增强MRI病灶的变化 ,有条件的最好每月检查 1次增强MRI。     

Induction of apoptosis by TPA and VP—16 is through translocation of TR3

AIM：To investigate the role of TR3 in induction of apoptosis in gastric cancer cells.METHODS:Human gastric cancer cell line,MGC80-3,was used .Expression of TR3 mRNA and its protein was detected by Northern blot and Western blot Localization of TR3 protein was showed by imunofluorescence analysis under laser-scanning confocal microscope.Apoptotic morthology was observed by DAPL fluorescence staining,and apoptotic index was couted manong 10000 cells randomly Stable transfection assay was carried out by Lipofectamine.RESULTS:Treatment of MGC80-3 cells with TPA and VP-16 resulted in apoptosis,accompanied by the repression of Bcl-2 protein in atime-dependent manner,At the same time,TPA and VP-16 also up-regulated expression level of TR3 mRNA in MGC80-3 cells that expressed TR3 mRNA When antisense-TR3 expression vector was transfected into the cells,expression of TR3 protein was repressed.In this case,TPA and VP-16 did not induce apoptosis.In addition,TPA and VP-16-induced apotosis involved in translocation of TR3,IN MGC80-3 cells,TR3 localized concentrative in nucleus,after treatment of cells with TPA and Vp-16,TR3 translocated from nuclesu to cytosol obviously,However,When this nuclear translocaion was blocked by LMB apoptosis was not occurred in MGC80-3 cells even in the presence of TPA and VP-16.CONCLUSION:Induction of apoptosis by TPA and VP-16 is through induction of TR3 expression and translocation of TR3 from nucleus to cytosol,Which may be a novel signal pathway for TR3,and represent the new biological function of TR3 to exert its effect on apoptosis in gastric cancer cells.     

Association of androgen receptor gene CAG and GGN repeat polymorphism with cryptorchidism: A meta‐analysis

Researches on association between variations in the androgen receptor (AR) gene repeat polymorphisms and cryptorchidism (CO) had conflicting results. The aim of this meta‐analysis was to analyse the potential effects of AR CAG and/or GGN repeat polymorphism on CO. Studies were independently appraised by two investigators on PubMed, Web of Science, EBSCO databases and Foreign Medical Retrieval System. Case–control studies with measurement of CAG and/or GGN repeat length were included. Weighted mean difference (WMD) and 95% confidence intervals (CIs) for the CAG or GGN repeat polymorphism and CO were calculated. Five reports were included in this analysis. Overall, no difference was identified between patients and fertile men in CAG repeat length. However, when the CO was divided into unilateral and bilateral, longer CAG repeat region was significantly associated with CO in bilateral group (WMD = 0.74; 95% CI, 0.01–1.47; p < .05). In addition, GGN lengths were significantly higher in patients compared with those in controls (WMD = 1.17; 95% CI, 0.28–2.06; p < .05). No obvious effect was found in the GGN length when compared unilateral or bilateral group with control respectively. The results in this meta‐analysis indicated that AR CAG and GGN repeat polymorphisms may be an important pathogenesis of CO.     

1例斯特奇-韦伯综合征合并脑梗死病人的护理

Sturge-Weber综合征又称斯特奇-韦伯综合征及脑面血管瘤病,主要病变为脑软膜血管瘤和毛细血管畸形,填充于蛛网膜下隙,病人容易发生脑出血及脑梗死等脑血管疾病,如果诊断及治疗不及时,可能危及病人生命及对病人将来的工作、学习和生活带来不良的影响。我科于2006年4月收治1例Sturge-Weber综合征合并脑梗死的病人。现将此例病人的护理介绍如下。