Substance P potentiates 5-HT3 receptor-mediated current in rat trigeminal ganglion neurons

The present study aimed to investigate the interaction between the coexistent SP receptor and 5-HT3 receptor in trigeminal ganglion (TG) neurons using whole-cell patch clamp technique. The majority of the neurons examined responded to 5-HT with an inward current (I5-HT) (78.2%, 79/101) that could be blocked by 5-HT3 receptor antagonist, ICS-205,930. The I5-HT was potentiated by preapplication of SP (10(-10) to 10(-8) M) in most 5-HT-sensitive cells(78.5%, 62/79). Coapplication of SP and GR-82334, antagonist of NK1 receptor, had no enhancing effect on I5-HT. The concentration-response curves for 5-HT with and without SP preapplication show that: (1) the threshold 5-HT concentrations with and without SP preapplication are basically the same, while SP preapplication increased the maximal value of I5-HT by 38.0% of its control; (2) the EC50 values of the curves with and without SP pretreatment are very close, i.e. 1.89 x 10(-5) M and 2.08 x 10(-5) M (P > 0.1; n = 9), respectively. Intracellular dialysis of GDP-beta-S, a non-hydrolyzable GDP analog, and GF-109203X, a selective protein kinase C inhibitor, removed the SP potentiation of I5-HT. These results may offer a clue to understanding the mechanism underlying the generation and/or regulation of peripheral pain caused by tissue damage inflammation, etc.     

1 MHz腔内射频加热辐射器在体模和犬颈段食管中的热场分布

目的 在肌肉等效体模及Beagle犬颈段食管中测定ZRL—Ⅱ型食管腔内加热辐射器的温度分布，探讨其热场分布能否满足食管癌加热治疗的临床需要。方法 ①在肌肉等效体模中测定ZRL—Ⅱ型食管腔内射频加热辐射器的温度分布，②Beagle犬用氯胺酮麻醉、固定。将腔内加热辐射器插入食管腔内，加热45min后分层解剖犬的颈部，同时测量食管外壁、气管、食管周围软组织中的温度。结果 ①在肌肉等效体模中距离导管囊表面1cm的环形体内的温度在43．2℃～43．6℃之间；在距离导管囊表面2cm的环形体内的温度在42．6℃～43．3℃之间，在距离导管囊表面3cm的环形体内的温度在42．6℃～42．8℃之间。②测得Beagle犬颈段食管腔内和外壁的温度为43．5℃，气管内为38．0℃，主动脉旁38．0℃，距食管外壁1cm处的软组织温度为40-3℃，距食管外壁2cm处的软组织温度为39．0℃，而颈部皮下的温度是37．5℃。结论 ①ZRL-Ⅱ型射频食管腔内加热辐射器的热场分布满足临床食管腔内加热的需要，②体模测定与活体动物测定，热场分布存在一定差异，需进一步研究。     

缺碘和氟中毒对大鼠甲状腺的协同作用

实验用Wistar大鼠212只,按饮食中碘和氟含量不同随机分为五组,实验期为7个月。结果显示:摄碘正常的大鼠长期饮用30ppm氟水后引起甲状腺功能和形态的严重损害,而饮用10ppm氟水的大鼠仅见甲状腺滤泡上皮超微结构的轻度异常变化;单纯性缺碘大鼠甲状腺肿大并伴有代偿性功能变化,缺碘并饮用10ppm氟水的大鼠在其甲状腺肿大的同时伴有明显的形态结构损伤及甲状腺代偿功能抑制,甚至功能低下。     

Association of genetic polymorphism in plasminogen activator inhibitor-1 gene with endometrial hypoplasia in infertile women

OBJECTIVE: To investigate the relationship between the plasminogen activator inhibitor (PAI-1) polymorphisms and endometrial hypoplasia in infertile women. METHODS: The study was conducted in 105 primary infertile patients with endometrial hypoplasia diagnosed by pathology and the thickness of endometrium by B-mode ultrasound and 85 controls who were not pregnant and had normal fertility. The -675 4G/5G polymorphism in the PAI-1 gene was detected by polymerase chain reaction-restriction fragment length polymerphim analysis. RESULTS: The frequencies of 4G/4G genotype and 4G allele of the PAI-1 gene were higher in the patient group (48.6% and 66.2%) than in the normal controls (22.4% and 47.1%) (P < 0.01). ThePAI-1 4G/4G genotype was significantly associated with endometrial hypoplasia in the infertile patients (OR=4.9, 95% CI: 2.10-10.12). CONCLUSION: The present findings suggest that the 4G/5G polymorphism of the PAI-1 gene was associated with endometrial hypoplasia in infertile patients.     

医疗用超硬质热塑性聚氨酯TPU的抗肿瘤药物溶剂耐受性研究

研究四种类型热塑性聚合物的6种常见医用输液接头材料对抗肿瘤药物溶剂的耐受性。将这些材料的标准测试样条固定在弯曲应变为1.5%的自制夹具上,分别浸泡在Etoposide?药物溶剂、Busulflex?药物溶剂或DMSO(以50%为例)等3种抗肿瘤药物溶剂,然后观察试样的外观,测试试样的拉伸强度、断裂伸长率和缺口冲击强度等性能的变化情况,采用定量化的评分,对6种材料的抗肿瘤药物溶剂的耐受性进行了评价。结果表明,两款超硬质热塑性聚氨酯(TPU)材料对三种抗肿瘤药物溶剂的耐受性均优于其他4种评价的材料,为接触抗肿瘤药物类的医疗器械产品的较优选择,比如PICC,CVC,输液器等。     

酮咯酸氨丁三醇滴眼地兔眼部炎症作用的研究

目的：研究酮咯酸氨丁三醇滴眼液（KTOS）对家兔化学性及外伤性眼部炎症的作用。方法：用巴豆油致炎液经角膜注入眼前房法制备兔眼部急性化学炎症模型;用重物击打眼睑法制备兔眼部急性外伤性炎症模型。将致炎后的动物随机分组给药,并参照眼部炎症评判标准进行评分统计。结果：各KTOS剂量组对外伤性和化学性眼部炎症作用的评分均低于生理盐水组;小剂量KTOS虽有作用,但无统计学意义（P＞0．05）,而中、大剂量KTOS均有明显改善外伤性和化学性眼部炎症的作用（P＜0．05,P＜0．01）;其中对化学性眼部炎症的作用与地塞米松作用相似,对外伤性眼部炎症的作用优于地塞米松。结论：KTOS对外伤性和化学性眼部炎症均有明显的抗炎作用,对外伤性眼部炎症的抗炎作用更强。     

Fractionated carbon dioxide (CO2) laser treatment contributes to trans-nail penetration of rhodamine B and changes of cytokine microenvironment

Lasers in Medical Science - This study is to determine the role of the fractional CO2 laser in topical drug delivery and the impact of local immune responses. Experimental rabbit nails were treated...     

Observation versus radiotherapy with or without temozolomide in postoperative WHO grade II high-risk low-grade glioma: a retrospective cohort study

The optimal adjuvant treatment of high-risk low-grade glioma (LGG) is controversial. We performed this retrospective cohort study to compare three treatments including observation, radiotherapy (RT) alone, and radiotherapy combined with concomitant and adjuvant temozolomide (TMZ) chemotherapy (STUPP regimen) in patients with high-risk LGG. Patients with high-risk (age > 40 or undergoing subtotal resection or biopsy) LGG treated with observation or radiotherapy alone or STUPP regimen after operation were retrospectively analyzed. Survival rates were evaluated by the Kaplan-Meier method; the log-rank test was applied to compare differences between groups. A total of 250 patients met the inclusion criteria. Median follow-up for living people was 70 months. Overall, patients who received radiotherapy with or without temozolomide had better progression-free survival (PFS) and overall survival (OS) when compared with observation (median PFS: observation, 59 months; RT, 82 months; STUPP, not reached; median OS: observation, 96 months; RT, not reached; STUPP, not reached), whereas STUPP regimen did not further prolong PFS or OS than RT alone (PFS, P = 0.203; OS, P = 0.146). In oligodendroglioma (IDH mutant and 1p/19q codeleted) subtype, only STUPP regimen brought longer PFS when compared with observation (P = 0.008). The incidence of grade 3 or 4 neutropenia (P < 0.001) and nausea or vomiting (P = 0.004) was higher in the STUPP group than the figure for the RT alone group. PFS and OS were similarly improved in patients with high-risk LGG receiving RT alone or STUPP regimen. However, only STUPP regimen was able to bring better PFS for oligodendroglioma (IDH mutant and 1p/19q codeleted) subgroup. Longer follow-up time is needed to determine an association with treatment effect in different histological and molecular subgroups.