行为干预及生物反馈治疗对老年高血压患者降压疗效的影响

目的 研究应用行为干预治疗及生物反馈治疗对老年高血压患者降压药物的影响。方法 在应用降压药物的基础上,增加行为干预及生物馈治疗,观察综合性行为干预措施对降压疗效的影响。结果 增加行为干预及生物反馈治疗后,降压和情绪障碍矫正的效果比单用药物明显改善。结论 老年高血压患者给予综合性行为干预措施可增加降压药物的疗效。     

Golytely肠道清洁法应用292例分析

本院自 1 993年初以来 ,用Ｇｏｌｙｔｅｌｙ液对2 92例结、直肠手术及纤维结肠镜检查病人作术前准备均取得较清单效果 ,现介绍如下。1　临床资料1 .1　 一般资料　本组患者共 2 92例 ,其中男性 1 85例 ,女性 1 0 7例。结、直肠手术 1 2 7例 ,诊断结肠肿瘤 72例 ,直肠肿瘤 5 5例。纤维结肠镜检查 1 6 5例。1 .2　配方及使用方法　Ｇｏｌｙｔｅｌｙ液配方〔1〕:聚乙二醇 40 0 0 5 9.1 ｇ,硫酸钠 5 .6 ｇ,氯化钠0 .75 ｇ,碳酸氢钠 1 .3ｇ加水至 1 0 0 0ｍｌ。使用方法 :术前 1ｄ下午服Ｇｏｌｙｔｅｌｙ ,饮速大于1 5 0 0ｍｌ/ｈ ,至排出清液止 …     

脑缺血再灌注期间海马CA1区微管运动蛋白活性变化

研究脑缺血再灌注期间海马ＣＡ１区锥体细胞微管运动蛋白活性的变化。方法沙土鼠前脑缺血再灌注,脑缺血１０ｍｉｎ。将２０只土鼠随机分为４组：假手术组、再灌注Ⅰ组（再灌注６ｈ）、再灌注Ⅱ组（再灌注４８ｈ）、再灌注Ⅲ组（再灌注９６ｈ）。采用免疫组织化学方法结合计算机图像分析测定脑缺血再灌注期间海马神经元微管运动蛋白活性。结果脑缺血再灌注期海马ＣＡ１区微管运动蛋白活性明显下降,在再灌注６ｈ,４８ｈ,９６ｈ时期     

二尖瓣脱垂的外科治疗

报道５７例二尖瓣脱垂（ＭＶＰ０不同术式的外科治疗效果。手术随机分为３组;传统的二尖瓣成形组,Ｇｏｒｅ－Ｔｅｘ线人工腱索替换组和瓣膜置换组。结果显示,Ⅱ组和Ⅲ组术后每搏二尖瓣返流量明显少于Ⅰ组。各组术后长期随访机能状况均明显改善。ＭＶＰ的外科治疗应首选二尖瓣成形;而Ｇｏｒｅ－Ｔｅｘ线人工腱索替换和人造瓣环盛开有方法安全可靠,其效果优于传统的腱索成形,并可避免瓣膜置换术后长期抗凝的并发症。     

异丙酚氯胺酮混合液静脉麻醉

选择 40例 ASA ～ 级病人行异丙酚氯胺酮 (1∶ 2 )复合静脉麻醉。结果麻醉诱导后血压、心率与麻醉前相比无明显变化 ,2 0 %病人出现一过性 SPO2 下降 (<94% )。麻醉诱导苏醒快 ,满意率高 ,恢复期无精神症状。表明异丙酚可控制氯胺酮的心血管兴奋作用和恢复期精神症状 ,氯胺酮可减轻异丙酚的心血管抑制作用。两者配伍是一种较好的短效静脉麻醉方式 ,但仍存在呼吸抑制作用 ,应引起注意。     

P53与ras癌基因产物对直肠癌的诊断价值

目的提高直肠癌术前活检的诊断率。方法5年来手术治疗的168例直肠癌病人中,有20例（11．9％）第一次手术前活检不能明确诊断。对其中17例患者的活检组织石蜡标本（实验组）和10例正常肠粘膜活检标本（对照组）进行常规病理、P53与ras癌基因产物检测。结果实验组中P53与ras基因表达阳性率分别为71％与47％,阳性率均高于常规病理,有显著性差异。17例标本中,11例为不典型增生,5例仅有慢性炎症。不典型增生的P53与ras基因表达分别为11例和7例,而慢性炎症则全部为阴性。二者之间,两种基因表达阳性率均有显著差异。实验结果还显示P53与ras基因表达有较好的一致性。对照组的10例正常肠粘膜的活检标本P53与ras基因检测结果均阴性,与实验组比较,P53阳性率有显著差异。结论P53与ras基因表达检测,可以作为常规病理的补充,提高直肠良恶性病变的鉴别力。     

光猝灭法测定水杨酸醑中的水杨酸

目的：探索一种新的测定水杨酸醑中水杨酸的方法。方法：采用光猝灭法测定水杨酸醑中的水杨酸。结果：水杨酸浓度在 １～４０ μｇ／ｍ ｌ 范围内与 ｌｇ（ Ｆ０／ Ｆ）成线性,检测限为 １０ μｇ／ｍ ｌ,回收率为 １０２％ , Ｒ Ｓ Ｄ 为７８５％ ,与紫外分光光度法比较没有显著性差异（ Ｐ＞ ０．０５）。结论：本实验为在位、在线测定水杨酸奠定了基础。     

CART: from gene to function

CART was identified as a novel mRNA regulated by psychostimulant drugs. CART peptides appear to be neurotransmitters involved in a variety of functions such as feeding. The mouse gene has been characterized and localized to Chromosome 13. The processing of CART peptides is evident in Western blotting studies.     

Expression of intercellular adhesion molecule 1 (ICAM-1) is reduced in permanent focal cerebral ischemic mouse brain using an adenoviral vector to induce overexpression of interleukin-1 receptor antagonist

Our previous studies have demonstrated that overexpression of recombinant human interleukin-1 receptor antagonist protein (IL-1ra) via gene transfer can reduce ischemic brain injury. However, the mechanism of action of IL-1ra in ischemia is unclear. Since interleukin-1 can up-regulate intercellular adhesion molecules in endothelium, the present study was designed to determine whether overexpression of the IL-1ra can reduce the expression of intercellular adhesion molecule-1 (ICAM-1) after ischemic injury. Normal saline or adenovirus vector (1x109 particles) encoding the human IL-1ra gene (Ad.RSVIL-1ra) or the Escherichia coli LacZ gene (Ad.RSVlacZ) was injected into the right lateral cerebral ventricle of adult CD-1 mice. After five days, permanent middle cerebral artery occlusion (MCAO) was achieved for 24 h using an intraluminal suture. Cerebral blood flow was monitored by transcranial laser Doppler flowmetry to verify the occlusion. ICAM-1 protein was quantified using Western blot analysis and localized using immunohistochemistry. After MCAO, surface blood flow in the ischemic hemisphere was decreased to 9-11% of the baseline. There were fewer ICAM-1 positive vessels in the ischemic cortex of the Ad.RSVIL-1ra transfected mice than in the Ad.RSVlacZ transfected and saline treated mice (138+/-19 vs. 249+/-25, 284+/-22, p<0.05). Western blot analysis shows that ICAM-1 protein decreased 50-60% in the Ad. RSVIL-1ra group compared to the other two groups. There were no significant differences in the numbers of positive vessels in the ischemic basal ganglia and contralateral hemisphere among the three groups. Our studies suggest that IL-1ra overexpression can down-regulate the expression of ICAM-1 in the ipsilateral cortex in ischemic mice. Interleukin-1 may play an important role in the activation of inflammatory reaction during focal cerebral ischemia by promoting leukocyte adhesion on the endothelium cells.     

40所军队医院病种病例分型质量评价结果分析

应用病例分型质量费用监控管理软件和ＳＡＳ６．１２统计软件包,对４０所军队医院５０万份病例样本资料进行深入分析,结果显示：病例分型组合数据稳定,分析结果合理。