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排序方式: 共有127条查询结果,搜索用时 15 毫秒
1.
Inactivation of C-1 inhibitor by proteases: demonstration by a monoclonal antibody of a neodeterminant on inactivated, non-complexed C-1 inhibitor. 总被引:4,自引:0,他引:4 下载免费PDF全文
Monoclonal antibodies were raised against kallikrein-C-1 inhibitor and factor XIIa-C-1 inhibitor complexes. One of the monoclonal antibodies (KII) appeared to react predominantly with C-1 inhibitor complexes in an ELISA. However, the apparent binding of KII to C-1 inhibitor complexes was probably due to the presence of proteolytically inactivated C-1 inhibitor in the complex mixture used for the coating:KII did not bind either kallikrein-C-1 inhibitor or factor XIIa-C-1 inhibitor complexes generated in plasma by dextran sulphate. SDS-PAGE analysis of C-1 inhibitor incubated with proteases revealed that KII-reactive C-1 inhibitor has a lower molecular weight than native C-1 inhibitor. We propose that the determinant that reacts with KII is exposed after cleavage of C-1 inhibitor in its reactive site. The monoclonal antibody KII will enable us to study the inactivation of C-1 inhibitor in human inflammatory disease. 相似文献
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Thirty NF1-patients (mean age 11.7 years, SD = 3.3) and 30 healthy controls (mean age 12.5 years, SD = 3.1) were assessed
on social skills, autistic traits, hyperactivity-inattention, emotional problems, conduct problems, and peer problems. Cognitive
control, information processing speed, and social information processing were measured using 5 computer tasks. GLM analyses
of variance showed significant group differences, to the disadvantage of NF1-patients, on all measures of behavior, social
functioning and cognition. General cognitive ability (a composite score of processing speed, social information processing,
and cognitive control) accounted for group differences in emotional problems, whereas social information processing accounted
for group differences in conduct problems. Although reductions were observed for group differences in other aspects of behavior
and social functioning after control for (specific) cognitive abilities, group differences remained evident. Training of cognitive
abilities may help reducing certain social and behavioral problems of children with NF1, but further refinement regarding
associations between specific aspects of cognition and specific social and behavioral outcomes is required. 相似文献
5.
Shawn E. Christ Stephan C.J. Huijbregts Leo M.J. de Sonneville Desirée A. White 《Molecular genetics and metabolism》2010
Despite early and continuous dietary intervention, individuals with early-treated phenylketonuria (PKU) experience significant neurocognitive sequelae. An area of cognitive ability that is believed to be particularly affected is executive function (EF). This paper provides a critical review of the evidence for EF impairment in early-treated PKU within the context of recent advances in neuropsychological theory and research. The most consistent findings of PKU-related EF impairment were in executive working memory and prepotent response inhibition. Surprisingly, findings on shifting ability and other more complex aspects of EF were largely equivocal. Cohort (e.g., age, phenylalanine (Phe) levels) and task (e.g., standard clinical versus experimental tasks) related differences likely contributed to the variability in findings reported by these studies. Day-to-day EF also appears to be impaired although the precise pattern of impairment remains unclear, as does the relationship between laboratory measures of EF and questionnaires assessing day-to-day EF. Similarly, whereas several studies have found a relationship between Phe levels and EF, the best predictor variable (e.g., concurrent Phe level, lifetime Phe level, Phe level variability) of current EF performance varied from study to study. Neurologic compromise related to dopamine deficiency, white matter abnormalities, and disruptions in functional connectivity likely underlies the EF impairments described in this review. In closing, this review identifies remaining unanswered questions and future avenues for research. 相似文献
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Calculating life-cycle assessment effect factors from potentially affected fraction-based ecotoxicological response functions 总被引:1,自引:0,他引:1
A new multisubstance potentially affected fraction (msPAF)-based method for calculating ecotoxicological effect factors for life-cycle assessment is introduced and compared to two other available methods of calculation. The new method is based on marginal increase of the msPAF of species. The method follows concentration-additive rules for pollutants with the same toxic mode of action (TMoA) and response-additive calculation rules for pollutants with independent action, and it combines a TMoA-specific factor, which is calculated differently in different methods, and a substance-specific factor, which is common to all methods. For 261 substances in 22 toxic modes of action, ecotoxicological effect factors for freshwater ecosystems have been calculated by different methods. Method-related differences appear to be rather small. Intersubstance differences in effect factors stem from differences in substance-specific toxic potencies, which span eight orders of magnitude, rather than from differences in TMoA-specific factors, which span only three orders of magnitude. Based on these insights, the choice of a calculation method seems to be a matter of personal (scientific) preference. The new hybrid msPAF method was greatly sensitive to data that usually are not known with sufficient certainty. Its use is recommended, entirely for reasons of scientific consistency, under strict conditions. When such conditions are not met or if necessary parameter values are unavailable, use of a fixed value for the TMoA-specific component is recommended. 相似文献
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Robin L. McKiernan Grgoire Cardoen Bernard Boutevin Bruno Amduri Samuel P. Gido Jacques Penelle 《Macromolecular chemistry and physics.》2003,204(7):961-969
Long‐chain heteroatom‐containing telechelic diols with 29–32 atoms in the backbone were synthesized by a one‐step, free‐radical telomerization of 10‐undecene‐1‐ol with commercially available α,ω‐dithiols. The oxygen and sulfur atoms caused a decrease in the melting point and enthalpy of the diols, compared to the corresponding purely aliphatic diols. The heteroatom‐containing α,ω‐diols HO? (CH2)11? S? (CH2)2? X? (CH2)2? S? (CH2)11? OH, where X = CH2, O, or O? (CH2)2? O, were reacted in the melt with 1,6‐diisocyanatohexane O?C?N? (CH2)6? N?C?O, producing a series of polyurethanes containing an increasing amount of heteroatoms. Characterization by differential scanning calorimetry, infra‐red spectroscopy, thermogravimetric analysis, and wide angle x‐ray scattering of the m,n‐polyurethane series showed that, like the telechelic diols they were synthesized from, the heteroatoms caused a decrease in the melting point and enthalpy. However, they did not affect either the decomposition temperature or the crystal structure/packing.
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Irmgard Henning-de Jong Ad M.J. Ragas Harrie W.M. Hendriks Mark A.J. Huijbregts Leo Posthuma Arjen Wintersen A. Jan Hendriks 《Ecotoxicology and environmental safety》2009,72(8):2037-2045
The present study aims to support decisions on whether or not to perform an extra toxicity test in order to improve environmental quality standards (EQSs). The impact of an additional ecotoxicity test was analyzed by predicting new ecotoxicity values with three different estimation methods and adding them to existing species sensitivity distributions (SSDs) on which the EQSs are based. The results show that EQSs are likely to increase due to increasing sample size, but the change also depends on the number of toxicity values available, the estimation method used and the representativeness of the species tested. The management consequences are illustrated in a case study on contaminated freshwater sediment in the Netherlands. It is shown that a slight increase of the EQS can result in a large reduction of sediment remediation costs without impairing regulatory protection levels. The paper identifies indicators that can be used to evaluate the potential impact of an extra ecotoxicity test. 相似文献
10.
J H Nuijens C C Huijbregts M Cohen G O Navis A de Vries A J Eerenberg J C Bakker C E Hack 《Thrombosis and haemostasis》1987,58(2):778-785
Radioimmunoassays (RIAs) for the detection of C-1-inhibitor (C-1-Inh) complexed to either kallikrein or activated Hageman factor (factor XIIa) are described. Kallikrein-C-1-Inh or factor XIIa-C-1-Inh complexes were bound to Sepharose to which monospecific antibodies against (pre)kallikrein or factor XII, respectively, were coupled. Bound complexes were subsequently detected by an incubation with affinity purified 125I-labeled antibodies against C-1-Inh. These RIAs were used to detect activation of the contact system of coagulation in vitro and in vivo. Addition of dextran sulfate (DXS) (20 micrograms/ml) to fresh plasma resulted at 37 degrees C in the rapid generation of amidolytic kallikrein activity, which was maximal after 1 to 2 min of incubation and subsequently decreased within a few minutes. The generation of kallikrein activity coincided with the appearance of both kallikrein-C-1-Inh and factor XIIa-C-1-Inh complexes. However, in contrast to kallikrein activity, both types of complexes remained detectable in the incubation mixtures during the incubation period. Experiments with purified kallikrein. C-1-Inh and partly purified beta-factor XIIa, and activation experiments in plasmas deficient in either factor XII or prekallikrein, demonstrated the specificity of both RIAs. The minimal amount of DXS that resulted in the generation of measurable amounts of both types of complexes in plasma was 2-3 micrograms per ml. Similar experiments with kaolin showed that with limiting amounts of activator (1-2 mg/ml), only kallikrein-C-1-Inh complexes were detected in plasma. When larger amounts of kaolin were added to plasma, factor XIIa-C-1-Inh complexes were additionally detected in plasma.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献