老年糖尿病患者腹腔镜胆囊切除术49例临床分析

目的分析总结老年糖尿病患者行腹腔镜胆囊切除术的可行性。方法分析49例老年糖尿病患者腹腔镜胆囊切除术的临床资料。结果全部患者均完成手术，2例合并戳孔感染。无死亡病例。结论对合并糖尿病的老年胆石症患者。应严格掌握手术适应证。术前充分准备，血糖需控制在10mmol／L以内。术中加强监控。可安全完成腹腔镜胆囊切除术。     

李振华教授调理脾胃用药规律探讨

李振华教授，生于1924年，河南省洛宁县人。著名中医脾胃学专家，全国片批名老中医。国务院特殊津贴专家，原中华中医药学会常务理事、第七届全国人大代表，曾任河南中医学院院长，从事中医临床与教学六十余载，长于内科杂病，晚年潜心于脾胃学说研究，著有《中国传统脾胃病学》等，并负责研究河南省重点科研项目“脾胃气虚本质的研究”及“七五”国家重点科技攻关项目“慢性萎缩性胃炎脾虚证临床及实验研究”，且均荣获河南省科技进步成果奖。经过多年临床实践，李老整理总结出调理脾胃的用药规律。提出通过药物件味归经，以及药效应用与脾胃的生理、病理特点相结合的川药大法。     

自体造血干细胞采集的护理

[目的]为进行自体外周血造血干细胞移植的病人提供优质护理，采集足够数量的造血干细胞，降低术中及术后不良反应。[方法]采用COBE Spectra血细胞分离机采集造血干细胞在采集前严密观察病人心理和血常规的变化，维持病人心理状态的稳定，掌握干细胞采集的最佳时机；术中严密观察生命体征、意识、四肢口唇是否有麻木感；术后监测病人外周的穿刺部位是否有出血，有无手足抽搐。[结果]20例病人均采集足够数量的造血干细胞，无不良反应发生：[结论]优质护理为自体外周血造血干细胞移植的成功奠定了基础。     

县级医院护士伦理道德素质调查

近年来，对护理伦理学的研究已日渐增多，护理伦理学已列入护理的正规教程，但时于基层医院护理队伍伦理道德素质状况的研究甚少，县级医院面对的是占中国80％的农村和城镇居民，其服务对象不同于大医院，也不同于村、镇卫生院。基于上述原因，本问卷调查目的在于丁解县级医院护理队伍伦理道德素质教育的状况。以及护士所面对的冲突和困难，以加强和改善护理队伍的素质建设，提高护理工作质量。     

Effective high-capacity gutless adenoviral vectors mediate transgene expression in human glioma cells.

Glioblastoma multiforme (GBM) is the most common subtype of primary malignant brain tumor. Although serotype 5 adenoviral vectors (Ads) have been used successfully in clinical trials for GBM, the capacity of Ads to infect human glioma cells and the expression of adenoviral receptors in GBM cells have been challenged. In this report, we studied the expression of three molecules that have been shown to mediate adenoviral entry into cells, i.e., coxsackie and adenovirus receptor (CAR), integrin alphavbeta3 (INT), and major histocompatibility complex class I (MHCI), in rodent glioma cell lines and low-passage primary cultures and cell lines from human GBM. We correlated levels of expression of CAR, INT, and MHCI with transduction efficiency elicited by several high-capacity helper-dependent adenoviral vectors (HC-Ads). Expression levels of adenoviral receptors were variable among the different GBM cells studied. HC-Ad-mediated therapeutic gene expression was efficient, ranging between 20 and 80% of the total target cells expressing the encoded transgenes. Our results show no correlation between the levels of CAR, INT, or MHCI molecules and the levels of transgene expression or the number of GBM cells transduced. We conclude that expression levels of adenoviral receptors do not predict their transduction efficiency or biological function.     

高等院校计算机基础教育的现状与对策

通过分析高等院校计算机基础教育面临的形势和存在的问题，提出相应的解决办法，同时指出教师必须采用新的教学模式和教学方法，这样才能培养出符合时代需求的新型人才。     

在音乐缭绕中痊愈

你是否曾经因为它血脉贲张浑身发抖过．你是否因为它心潮起伏热泪盈眶过？如果这世界上没有它．你怕么？当天堂的圣歌响起．即使面对死亡．你也无所畏惧，它以它温柔的手．抚平你心里和肉体的伤……它让你不痛。[编者按]     

高护生临床授课效果评价

高等护理毕业生应具备临床护理、护理教育、护理科研、护理管理、社区护理5方面的能力。其授课水平的高低将成为评价高护生护理教育能力的重要指标。我院从2002年起探讨对高护生临床授课的教学模式，以提高授课水平。笔者对2004年-2005年在我院实习的168名高护生进行调查，现将结果报告如下。     

Novel pore mutation in SCN5A manifests as a spectrum of phenotypes ranging from atrial flutter, conduction disease, and Brugada syndrome to sudden cardiac death

OBJECTIVES: The purpose of this study was to determine the clinical and biophysical characteristics of a novel SCN5A mutation. BACKGROUND: Brugada syndrome and isolated cardiac conduction defect have been linked to SCN5A mutations. METHODS: Eleven members of a western European family underwent electrophysiologic investigations and mutation analysis of the SCN5A gene. Wild-type and mutant SCN5A channels were expressed in HEK293 cells, and whole cell currents were studied using patch clamp procedures. RESULTS: A novel mutation, R376H, in the first pore segment of SCN5A variably causes Brugada syndrome and/or conduction disease in a single family. Biophysical analysis demonstrated a significant current reduction for the mutant, a pathophysiologic profile consistent with Brugada syndrome and isolated cardiac conduction defect. Among 11 family members, 9 were carriers of the mutation. The proband's initial presentation was a saddleback Brugada ECG, atrial flutter, and diffuse conduction disturbances. He had no inducible ventricular arrhythmias but experienced sudden cardiac death. His brother was affected by atrial flutter and had a clear conduction disorder, but he did not display baseline or evocable ECG signs of Brugada syndrome. He received an implantable cardioverter-defibrillator that delivered one appropriate shock after 1 year of follow-up. The phenotype in the family members was highly variable and ranged from noninducible and inducible asymptomatic carriers of the mutations to isolated conduction disease and to symptomatic Brugada syndrome. CONCLUSIONS: We describe the functional characterization of a novel SCN5A pore mutation, R376H, with variable clinical expression in the same family. Differentiating between electrophysiologic entities (Brugada syndrome-isolated cardiac conduction defect) is more challenging. Recognition of factors modifying the clinical presentation may be important for clinical decision making.     

Mind-Refreshing Acupuncture Therapy for Facial Spasm, TrigeminalNeuralgia and Stubborn Facial Paralysis

Facial spasm, trigeminal neuralgia and stubborn facial paralysis are commonly seen in clinic. The authors have obtained quite good therapeutic results for the above diseases by using the mind-refreshing acupuncture therapy. These are introduced in the following.