成纤维细胞生长因子对兔纤维环细胞转化生长因子β2的调节

目的:观察成纤维细胞生长因子对培养兔纤维环细胞转化生长因子β2表达的调节作用。方法:实验于2005-03/2006-04在华中科技大学同济医学院附属协和医院骨科实验室完成。培养4月龄雌性日本大白兔的纤维环细胞,在每次换液时加入成纤维细胞生长因子100μg/L,铺满后收集细胞,作纤维环细胞转化生长因子β2常规免疫组织化学研究,细胞呈棕黄色染色为阳性表达信号,同时为进一步明确纤维环细胞转化生长因子β2的定位进行免疫胶体金标记电镜观察。结果:光镜下细胞明显呈棕色,电镜下细胞膜表面有明显的胶金颗粒粘附。结论:成纤维细胞生长因子能促进纤维环细胞转化生长因子β2的表达。     

Morgagni: His book, vision and views on hepatic coma

The study of pathological anatomy reached a culmination in the magnum opus of Morgagni, The Seats and Causes of Diseases. It represented an innovative way of looking at the patient and his illness, a change that Foucault described as occurring in the 18th century. Physicians saw symptoms penetrating the body and converging upon an organ. We suggest that Morgagni and his fellow workers derived their vision from the practice of visual perspective discovered by Italian Renaissance artists. Perceiving in three dimensional terms, Morgagni added spatial depth to our understanding of disease. His acute vision was brilliantly displayed in his views of hepatic coma. He was among the first to provide the clinical-pathological associations of acute liver failure. The limitations of gross morphology precluded a complete elucidation of the disorder.     

Anxiolytic property of Lactuca sativa,effect on anxiety behaviour induced by novel food and height

ObjectiveTo study anxiolytic property of hydro alchohol extract and to estimate polyphenols present in the extract by HPLC.MethodsTo evaluate anxiolytic property two animal models were used viz. Elevated T maze and hyponeophagia. Diazepam (1 mg/kg body wt.) served as the standard anxiolytic agent for all the tests. The dried extract of the plant leaf in doses of 100, 200 and 400 mg/kg body weight was administered orally to mice for duration of 15 or 30 days and locomotor and anxiolytic activities were performed. Polyphenols was estimated using HPLC.ResultsThe HPLC analysis of the polyphenols revealed the presence chlorogenic acid, vanillin, epicatechin, caffeic acid, rutin hydrate, sinapic acid, quercetin-3-rhamnoside, p-coumeric acid and quercitin. Time spent and number of entries into the open arm was improved in 30 days treated animals than that of 15 days treated groups, 200 and 400 mg/kg body weight treated group showed significant results when comparing with the control group.ConclusionsThe hydro alcohol extract rich in Polyphenols and other secondary metabolites is a potent anxiolytic agent.