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721.
AA da Silva L Bingle PM Speight CD Bingle T Mauad LFF da Silva PA Vargas 《Oral diseases》2011,17(3):258-264
Oral Diseases (2011) 17 , 258–264 Objective: To analyse and compare the expression of Palate, Lung, and Nasal Epithelium Clone (PLUNC) proteins in salivary glands from patients with and without AIDS (control group) using autopsy material. Methods: We analysed the expression of PLUNCs using immunohistochemistry in parotid (n = 45), submandibular (n = 47) and sublingual gland (n = 37) samples of AIDS patients [30 with normal histology, 21 with mycobacteriosis, 14 with cytomegalovirus (CMV) infection, 30 with chronic non‐specific sialadenitis, and 30 HIV‐negative controls. In situ hybridization (ISH) for SPLUNC 2 in the HIV‐negative group was performed. Results: SPLUNC 1 expression was detected in the mucous acini of submandibular and sublingual glands, and SPLUNC 2 were seen in the serous cells. LPLUNC 1 expression was only positive in the salivary ducts. There was a higher expression of SPLUNC 2 in AIDS patients with CMV infection and mycobacteriosis when compared with all other groups. The intensity of staining for SPLUNC 2 was greater around the lesions than the peripheral ones. ISH for SPLUNC 2 showed perinuclear positivity in the serous cells in all HIV‐negative cases. Conclusions: SPLUNC 1 and LPLUNC 1 proteins were similarly expressed in the salivary glands of AIDS patients and non‐HIV patients. CMV infection and mycobacteriosis increase SPLUNC 2 expression in serous cells in the salivary gland of AIDS patients. 相似文献
722.
Hanekamp MN Mazer P van der Cammen-van Zijp MH van Kessel-Feddema BJ Nijhuis-van der Sanden MW Knuijt S Zegers-Verstraeten JL Gischler SJ Tibboel D Kollée LA 《Critical care (London, England)》2006,10(5):R127-11
Introduction
Extracorporeal membrane oxygenation (ECMO) is a supportive cardiopulmonary bypass technique for babies with acute reversible cardiorespiratory failure. We assessed morbidity in ECMO survivors at the age of five years, when they start primary school and major decisions for their school careers must be made. 相似文献723.
BM McDermott AA Mamun JM Najman GM Williams MJ O'Callaghan W Bor 《Acta paediatrica (Oslo, Norway : 1992)》2010,99(1):68-71
Aim: To report the stability of parent-perceived child irregular eating from 6 months to 14 years of age and to investigate a predictive model inclusive of child and parent factors.
Methods: Of the 7223 singleton children in a birth cohort, 5122 children were re-interviewed at 5 years and 4554 for the 14-year analysis. Information was obtained from structured interviews including questions answered by parents of the child at birth, 6 months, 5 years and 14 years; and by teenagers at age 14 years and from physical measures of the child. The mother's perception that the child was an irregular eater at age 14 years was the major outcome variable of interest.
Results: Approximately 40% of irregular eaters at age 5 will still be irregular eaters at age 14 years. This was not related to maternal education or socio-economic class. Significant at multivariate analysis were infant feeding problems and the children's ability to regulate their sleep and mood. Significant maternal factors were greater age, not feeling positive about the baby and persistent maternal anxiety during the child's early years.
Conclusion: Irregular eating behaviour displays considerable continuity from childhood to mid-adolescence. Independent contributions to this behavioural phenotype include child biological and psychological factors and maternal anxiety during the child's early years. 相似文献
Methods: Of the 7223 singleton children in a birth cohort, 5122 children were re-interviewed at 5 years and 4554 for the 14-year analysis. Information was obtained from structured interviews including questions answered by parents of the child at birth, 6 months, 5 years and 14 years; and by teenagers at age 14 years and from physical measures of the child. The mother's perception that the child was an irregular eater at age 14 years was the major outcome variable of interest.
Results: Approximately 40% of irregular eaters at age 5 will still be irregular eaters at age 14 years. This was not related to maternal education or socio-economic class. Significant at multivariate analysis were infant feeding problems and the children's ability to regulate their sleep and mood. Significant maternal factors were greater age, not feeling positive about the baby and persistent maternal anxiety during the child's early years.
Conclusion: Irregular eating behaviour displays considerable continuity from childhood to mid-adolescence. Independent contributions to this behavioural phenotype include child biological and psychological factors and maternal anxiety during the child's early years. 相似文献
724.
Greater numbers of human spermatozoa associate with endosalpingeal cells derived from the isthmus compared with those from the ampulla 总被引:2,自引:3,他引:2
Baillie HS; Pacey AA; Warren MA; Scudamore IW; Barratt CL 《Human reproduction (Oxford, England)》1997,12(9):1985-1992
A simple co-culture bioassay system was used to investigate whether or not
the anatomical origin affected the ability of epithelial cells from the
human uterine (Fallopian) tube to 'bind' spermatozoa. This study was also
used to identify some of the factors which may be involved in the
regulation of sperm-epithelial interactions in vitro by comparing different
tissue culture models and assessing the effect of oestradiol concentration.
Epithelial explants harvested from different regions of human uterine tubes
were co-incubated with a known concentration of motile donor spermatozoa.
All results were adjusted to reflect a standard sperm concentration of 5 x
10(6)/ml. More spermatozoa associated per field of isthmic compared to
ampullary epithelium [isthmus 9.5 +/- 0.9, ampulla 7.1 +/- 0.7 (mean +/-
SEM); n = 36, P < 0.05, ANOVA] and cells from post-menopausal patients
had an apparently reduced ability to bind spermatozoa [isthmus 5.5 +/- 2.0,
ampulla 4.3 +/- 1.4 (mean +/- SEM); n = 4]. Neither menstrual cycle stage
nor addition of mid-cycle concentrations of 17beta-oestradiol (750 pmol/l)
affected the number of spermatozoa which bound to epithelium from either
tubal region. In addition, the number of spermatozoa which bound per field
of polarized explants was greater (P < 0.05) than that bound to
dissociated primary and passaged epithelial cell monolayers. This report is
the first to provide evidence suggestive of a role for sperm- epithelial
binding in the formation of an isthmic sperm reservoir in the human uterine
tube. Results also indicate that oestrogen is not involved in the
regulation of these interactions, and that cell polarity is an important
factor for such associations in vitro.
相似文献
725.
Background
Perception of midline diastema differs among individuals and cultures. To some, it is a feature of beauty, while to others it is aesthetically appalling. From a scientific point of view, midline diastema is a deviation from normal adult dentition. It is not uncommon among some African natives to find individuals demanding for artificially created midline diastema from dental practitioners.Objective
To present a rare case of pulpal necrosis involving maxillary and mandibular central incisors as a result of poorly executed teeth modification in a bid to create upper and lower midline diastemata.Result
A 23-year old lady presented with history of spontaneous, sharp, persistent pain associated with palatal swelling and discharging sinus of two weeks duration in relation to maxillary central incisors (11, 21). The complaint was preceded by a history of recurrent pain in relation to 11, 21, 31 and 41 which started soon after obtaining artificially created maxillary and mandibular midline diastemata from a dental quack. Periapical x-ray revealed periapical radiolucency in relation to 21 and 41. Vitality test confirmed necrotic pulp in the lower central incisors. The affected teeth were scheduled for root canal therapy (RCT) and porcelain fused to metal crown. The RCT of 11 and 21 had been completed but further treatment stalled due to financial constraint.Conclusion
This is a case of obsession for midline diastema, poor dental health education, and illegal dental practice. 相似文献726.
BACKGROUND: The study aimed to determine whether allergic conditions accompany analgesic intolerance. METHODS: A total of 132 analgesic-intolerant patients with bronchial asthma admitted to the adult allergy unit from January 1991 to October 1997 and 103 patients with bronchial asthma randomly selected from among the asthmatics referred to our department between January and October 1997 were enrolled in the study. Those having analgesic intolerance and bronchial asthma were accepted as group I; patients having only asthma were accepted as group II. A standard questionnaire was completed for all the patients. Physical examination, routine skin prick tests, determination of total IgE levels and blood type, and oral analgesic provocation tests were also performed. RESULTS: The results showed that some allergic conditions were significantly more common in group I (22.7% and 7.8% for food allergy/intolerance [P<0.05], 16.7% and 7.8% for antibiotic allergy, 16.7% and 2.9% for dermographism, 9.8% and 1.0% for metal allergy, and 9.1% and 1.0% for chronic urticaria for groups I and II, respectively [P<0.001]). In addition, the mean of the total IgE level in the serum was higher in group I than group II (77.6 and 53.7 IU/ml; P<0.05), and the cumulative analgesic consumption was more in group I (14.2+/-17.1 and 9.1+/-12.5 boxes; P<0.05). CONCLUSIONS: Dermographism; chronic urticaria; antibiotic, metal, and food allergy; high levels of total IgE; and a high amount of cumulative analgesic consumption may be the conditions accompanying analgesic intolerance in asthmatics. 相似文献
727.
We have shown recently that adenosine deaminase (ADA)-deficient mice die
perinatally with severe liver cell degeneration. In addition to enzyme
substitution, we report the restoration of viability through introduction
of the human ADA gene. The ADA gene is subject to complex developmental and
tissue-specific regulation. To include the cis- regulatory elements
necessary for correct regulation of the human ADA gene, a large transgenic
locus constituting the human ADA gene with 10 kb of 5' and 4 kb of 3'
flanking sequences was generated by co- injection of two overlapping DNA
fragments into murine zygotes. Probably as a result of extrachromosomal
(homologous) recombination between the fragments, one of the two transgenic
lines contained a reconstituted, functional human ADA gene. As in man,
human ADA expression generally was low in these transgenic mice, but high
in the thymus, spleen and gastro-duodenal part of the gut. Apparently, all
cis- regulatory elements essential for a human expression pattern were
incorporated in the transgene and were functional in the murine background.
Similarly to man, the upper alimentary tract of the transgenic mice
revealed low human ADA activity in contrast to extremely high levels of
murine ADA. The human gene probably lacks the cis-regulatory elements that
target high level murine ADA expression to the murine upper alimentary
tract. ADA-deficient mice rescued by introduction of the human ADA
transgene appeared histologically and immunologically normal. Apparently,
human ADA can complement murine ADA in all tissues, even in the epithelium
of the upper alimentary tract where human ADA activity is as much as
70-fold lower than murine ADA activity in wild-type mice. Clearly, the
lethal phenotype of ADA- deficient mice is due to the absence of ADA.
相似文献