全文获取类型
收费全文 | 2424篇 |
免费 | 189篇 |
国内免费 | 28篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 98篇 |
妇产科学 | 54篇 |
基础医学 | 357篇 |
口腔科学 | 40篇 |
临床医学 | 425篇 |
内科学 | 385篇 |
皮肤病学 | 24篇 |
神经病学 | 158篇 |
特种医学 | 168篇 |
外科学 | 204篇 |
综合类 | 31篇 |
预防医学 | 278篇 |
眼科学 | 27篇 |
药学 | 235篇 |
中国医学 | 3篇 |
肿瘤学 | 145篇 |
出版年
2023年 | 9篇 |
2022年 | 10篇 |
2021年 | 47篇 |
2020年 | 31篇 |
2019年 | 45篇 |
2018年 | 47篇 |
2017年 | 51篇 |
2016年 | 46篇 |
2015年 | 45篇 |
2014年 | 62篇 |
2013年 | 85篇 |
2012年 | 113篇 |
2011年 | 138篇 |
2010年 | 60篇 |
2009年 | 87篇 |
2008年 | 128篇 |
2007年 | 141篇 |
2006年 | 135篇 |
2005年 | 142篇 |
2004年 | 112篇 |
2003年 | 108篇 |
2002年 | 96篇 |
2001年 | 44篇 |
2000年 | 36篇 |
1999年 | 41篇 |
1998年 | 49篇 |
1997年 | 45篇 |
1996年 | 43篇 |
1995年 | 36篇 |
1994年 | 39篇 |
1993年 | 26篇 |
1992年 | 33篇 |
1991年 | 35篇 |
1990年 | 32篇 |
1989年 | 45篇 |
1988年 | 53篇 |
1987年 | 31篇 |
1986年 | 35篇 |
1985年 | 42篇 |
1984年 | 29篇 |
1983年 | 16篇 |
1982年 | 18篇 |
1981年 | 10篇 |
1980年 | 18篇 |
1979年 | 22篇 |
1978年 | 8篇 |
1977年 | 11篇 |
1976年 | 14篇 |
1973年 | 9篇 |
1969年 | 9篇 |
排序方式: 共有2641条查询结果,搜索用时 15 毫秒
11.
12.
Magali Louis Anne France Dekairelle Jean-Luc Gala 《Clinical chemistry and laboratory medicine》2004,42(12):1364-1369
Risk factors for cardiovascular diseases and venous thromboembolism involve both acquired and hereditary conditions. Among the latter, mutations in genes coding for coagulation factors (factor V Leiden [Arg506Gly], G20210A in the 3'-untranslated region of factor II ) and variant C677T of the methylenetetrahydrofolate reductase (MTHFR ) are often involved and co-inherited. These three factors were genotyped simultaneously in the same 96-well plate, using a real-time polymerase chain reaction (PCR) Taqman assay and minor groove binding DNA oligonucleotides (MGB probes). While primers and MGB probes matched their corresponding single nucleotide polymorphism (SNP), the real-time MGB program was identical for each target gene. Homozygous wild-type (WT; -/-), heterozygous (+/-) or homozygous (+/+) variants (n = 362) were selected for factor V (n = 115, with -/-, 40; +/-, 40; +/+, 35), factor II (n = 122, with -/-, 60; +/-, 60; +/+, 2), and MTHFR (n = 120, with -/-, 40; +/-, 40; +/+, 40), according to the results of conventional PCR-restriction fragment length polymorphism (PCR-RFLP), but the allelic discrimination was performed blind. Results of the real-time MGB and PCR-RFLP assays were identical. This new assay was easy and fast with high throughput, without risk of molecular carryover, and cost-effective for laboratories utilizing the Taqman or related fluorescence reading methods. These advantages make it particularly suitable for large-scale combined genotyping of several polymorphisms in the routine setting. 相似文献
13.
Release of soluble transferrin receptor from the surface of human leukemic HL60 cells 总被引:2,自引:0,他引:2
Information regarding transferrin (Tf) receptor degradation is largely incomplete. HL60 cells were shown to release to their growth medium a Tf-binding protein which could be immunoprecipitated by anti-Tf receptor monoclonal antibodies (MoAbs) B3/25 and OKT9. Soluble Tf receptor was detected in the medium within one hour of replating of cells, and its release was inhibited at 4 degrees C. The affinity of Tf for the soluble receptor released by cells (kd = 2.3 x 10(-10) mol/L) was slightly lower than its affinity for the detergent-solubilized cellular receptor (kd = 1.2 x 10(-10) mol/L). 125I-Tf internalized and released by cells subsequently bound to Tf receptor released by the same cells, and soluble Tf receptor in the conditioned medium (CM) inhibited 125I-Tf binding to intact cells. The soluble Tf receptor isolated from the CM was smaller (78,000 daltons) than the cell surface receptor (94,000 daltons) when analyzed by gel electrophoresis under reducing conditions. Isolated cell membranes readily released soluble receptor; however, this release could be blocked by protease inhibitors. The soluble Tf receptor may represent the extracytoplasmic domain of the cellular Tf receptor released from the surface of HL60 cells through proteolytic cleavage by a membrane-based protease. 相似文献
14.
Radiographic assessment of instability of the knee due to rupture of the anterior cruciate ligament. A quadriceps-contraction technique 总被引:2,自引:0,他引:2
J L Franklin T D Rosenberg L E Paulos E P France 《The Journal of bone and joint surgery. American volume》1991,73(3):365-372
We compared the results of a radiographic technique for the measurement of instability of the knee with those obtained with a KT-1000 arthrometer. The study was conducted on both knees of sixty patients who had a ruptured anterior-cruciate ligament in one knee, as well as in ten control subjects. The radiographic technique included the examination of a true lateral radiograph, made while the knee was in full extension and the quadriceps was maximally contracted, with a 66.7-newton downward force produced by a 6.8-kilogram weight suspended from the ankle. As demonstrated by both techniques, the maximum difference between the displacements of the right and left knees in the control subjects was 2.5 millimeters and the mean difference between the displacements in the two knees in the patients was 7.5 millimeters. In fourteen of the sixty knees in which the ligament was ruptured, the injury was acute. The forward translation of the medial side in these fourteen knees was compared with that in the forty-six knees in which the injury was chronic. The mean difference in the displacement of the medial side in the right and left knees was 3.5 millimeters in the fourteen patients who had an acute injury and 5.0 millimeters in the forty-six patients who had a chronic injury. Thirteen of the sixty patients had disruption of the posteromedial corner of the injured knee, and the translation of the medial side in these knees was significantly increased compared with that in the intact knees of the same patients.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
15.
16.
Daniel Van Gansbeke Thierry M. Metens Celso Matos Nicole Nicaise France Gay Hubert Raeymaekers Julien Struyven 《Journal of magnetic resonance imaging : JMRI》1997,7(3):482-489
This study was devoted to tumor differentiation in liver MR T1-weighted imaging with superparamagnetic iron oxide (SPIO). Twenty-one patients with 40 liver lesions were studied at 1.5 T. Before and at least 45 minutes after SPIO administration, turbo-field-echo (TFE) T1-weighted, TFE T1 × T2*-weighted (MXT), and fat-suppressed turbo-spin-echo T2-weighted images were acquired. A quantitative analysis was performed blindly. On TFE T1-weighted images, the signal enhancement was ?33% ± 12 for the liver, ?24% ± 2 for adenomas and focal nodular hyperplasia, +60% ± 33 for the hemangiomas; metastases and cyst enhancement were not significant. After SPIO on TFE T1-weighted images, the hemangioma-to-liver signal ratio (149% ± 18) was definitely higher than the mean metastasis-to-liver signal ratio (90% ± 16). This T1-related differentiation ability lacked dramatically on TFE MXT images and, in one case, was reduced on post-SPIO TFE T1-weighted images by a long imaging delay after SPIO administration (2 hours). 相似文献
17.
Prior studies have shown that pneumothorax is one of the more difficult entities to diagnose with digitized radiography. This study was designed to test whether increasing resolution from 1.25 to 2.5 line pairs per millimeter (lp/mm) and image processing (edge enhancement from unsharp masking) would increase accuracy and confidence in the diagnosis of pneumothorax, as well as normal cases and other forms of lung disease. Conventional radiographs were digitized with use of a laser reader and then reformatted as film hard copy. Eleven observers read 35 cases reformatted in three different ways (1.25 lp/mm, 2.5 lp/mm, 1.25 lp/mm unsharp mask). The images with finer resolution (2.5 lp/mm) and unsharp mask images were superior to those with coarser resolution (1.25 lp/mm) for the diagnosis of pneumothorax. There was no difference in diagnostic accuracy for normal patients. For abnormalities other than pneumothorax, the unsharp mask images were significantly worse. Confidence in the diagnosis of pneumothorax and other abnormalities was highest with the finest resolution (2.5 lp/mm). 相似文献
18.
Endothelin-1 in children with chronic renal failure 总被引:1,自引:0,他引:1
Isabelle Blazy Michèle Déchaux Marina Charbit Danièle Brocart Jean-Claude Souberbielle Marie France Gagnadoux François Guillot Charles Sachs 《Pediatric nephrology (Berlin, Germany)》1994,8(1):40-44
Endothelin-1 (ET-1) was meansured after extraction from plasma of normal adults (5.9±1.9 pg/ml,n=22), normal children (7.1±1.86 pg/ml,n=29), nonhaemodialysed children with chronic renal failure (CRF) (11.1±1.8 pg/ml),n=10), renal graft recipients (9.5±3.4 pg/ml,n=37), haemodialysed children 24 h after a haemodialysis session (20.02±10.9 pg/ml,n=26) and haemodialysed children before and after a haemodialysis session (15.31±10.6 and 13.8±8.5 respectively,n=14). A sensitive and specific radioimmunoassay was used. ET-1 was significantly higher in non-haemodialysed CRF children and in renal graft recipients than in normal children (P<0.001 andP<0.01, respectively) and significantly higher in haemodialysed children when compared with normal children, non-haemodialysed CRF children and renal graft recipients (P<0.001). ET-1 concentrations were similar in normal children and normal adults. ET-1 was inversely correlated with glomerular filtration rate in non-haemodialysed CRF children (r=–0.39,P<0.01) and positively correlated with extracellular volume in haemodialysed children (r=0.435,P<0.03). After haemodialysis, ET-1 increased in 6 and decreased in 8 of the 14 children studied before and after a haemodialysis session. 相似文献
19.
20.