全文获取类型
收费全文 | 898篇 |
免费 | 33篇 |
国内免费 | 19篇 |
专业分类
耳鼻咽喉 | 7篇 |
儿科学 | 36篇 |
妇产科学 | 5篇 |
基础医学 | 99篇 |
口腔科学 | 28篇 |
临床医学 | 64篇 |
内科学 | 138篇 |
皮肤病学 | 20篇 |
神经病学 | 67篇 |
特种医学 | 121篇 |
外科学 | 71篇 |
综合类 | 10篇 |
预防医学 | 53篇 |
眼科学 | 111篇 |
药学 | 62篇 |
中国医学 | 2篇 |
肿瘤学 | 56篇 |
出版年
2020年 | 6篇 |
2019年 | 14篇 |
2018年 | 13篇 |
2017年 | 13篇 |
2016年 | 10篇 |
2015年 | 14篇 |
2014年 | 14篇 |
2013年 | 33篇 |
2012年 | 17篇 |
2011年 | 15篇 |
2010年 | 34篇 |
2009年 | 29篇 |
2008年 | 17篇 |
2007年 | 22篇 |
2006年 | 21篇 |
2005年 | 29篇 |
2004年 | 30篇 |
2003年 | 26篇 |
2002年 | 27篇 |
2001年 | 15篇 |
2000年 | 23篇 |
1999年 | 17篇 |
1998年 | 34篇 |
1997年 | 38篇 |
1996年 | 30篇 |
1995年 | 27篇 |
1994年 | 26篇 |
1993年 | 18篇 |
1992年 | 9篇 |
1991年 | 14篇 |
1990年 | 17篇 |
1989年 | 23篇 |
1988年 | 34篇 |
1987年 | 20篇 |
1986年 | 30篇 |
1985年 | 25篇 |
1984年 | 17篇 |
1983年 | 17篇 |
1982年 | 17篇 |
1981年 | 8篇 |
1980年 | 5篇 |
1979年 | 11篇 |
1978年 | 5篇 |
1976年 | 7篇 |
1975年 | 14篇 |
1974年 | 9篇 |
1973年 | 7篇 |
1972年 | 12篇 |
1971年 | 5篇 |
1970年 | 8篇 |
排序方式: 共有950条查询结果,搜索用时 15 毫秒
71.
Vincent MC Gallai R Olivier D Speeg-Schatz C Flament J Calvas P Dollfus H 《American journal of ophthalmology》2004,138(6):1016-1021
PURPOSE: Several ocular defects have been identified as a consequence of the PAX6 gene mutations. With regard to the implication of this gene in unusual phenotypes, we report a family presenting with congenital nystagmus, foveal hypoplasia, and iris hypoplasia or atypical coloboma. DESIGN: Observational case report. METHODS: The entire transcribed region of the PAX6 gene was submitted to mutation search at the DNA and mRNA levels in five affected members of a French family in test with 82 normal subjects. RESULTS: A novel heterozygous PAX6 gene splice mutation (IVS4 + 5G>C) was identified. The mutation is located in IVS4 within the consensus donor splice site. A mutant mRNA lacking exon 4 as the sole defect was evidenced. The resultant protein was predicted to contain a cryptic ATG initiation codon in exon 3 and a slightly altered paired-domain in an open reading frame extended by 13 amino acids. CONCLUSIONS: The association of anterior segment anomalies and foveal hypoplasia with one of the slightest alterations of the PAX6 protein described to date confirms the association of variant phenotypes with hypomorphic alleles. Mutation screening of the PAX6 gene could be useful in elucidating the origin of complex ocular malformations. 相似文献
72.
Chaput N Andre F Schartz NE Flament C Angevin E Escudier B Zitvogel L 《Bulletin du cancer》2003,90(8-9):695-698
Exosomes are 60 to 90 nm membrane vesicles originating from late endosomes and secreted from most hematopoietic and epithelial cells in vitro. B cell derived-exosome antigenicity was first reported in 1996 in MHC class II restricted CD4+ T lymphocytes. In 1998, we reported that dendritic cell derived-exosomes are immunogenic in mice leading to tumor rejection. These findings have renewed the interest in exosomes. The current challenge consists in understanding the mechanisms and the physiological relevance of exosomes that could contribute to the design of the optimal exosome based-vaccination. Here, we will focus on the biological features pertaining to dendritic cell- and tumor cell derived-exosomes and will discuss their potential clinical implementation. 相似文献
73.
Neurofibrillary degeneration of the Alzheimer-type: an alternate pathway to neuronal apoptosis? 总被引:7,自引:0,他引:7
Hamdane M Delobel P Sambo AV Smet C Bégard S Violleau A Landrieu I Delacourte A Lippens G Flament S Buée L 《Biochemical pharmacology》2003,66(8):1619-1625
Neuronal death is a process which may be either physiological or pathological. Apoptosis and necrosis are two of these processes which are particularly studied. However, in neurodegenerative disorders, some neurons escape to these types of death and "agonize" in a process referred to as neurofibrillary degeneration. Neurofibrillary degeneration is characterized by the intraneuronal aggregation of abnormally phosphorylated microtubule-associated Tau proteins. A number of studies have reported a reactivation of the cell cycle in the neurofibrillary degeneration process. This reactivation of the cell cycle is reminiscent of the initiation of apoptosis in post-mitotic cells where G1/S markers including cyclin D1 and cdk4/6, are commonly found. However, in neurons exhibiting neurofibrillary degeneration, both G1/S and G2/M markers are found suggesting that they do not follow the classical apoptosis and an aberrant cell cycle occurs. This aberrant response leading to neurofibrillary degeneration may be triggered by the sequential combination of three partners: the complex Cdk5/p25 induces both apoptosis and the "abnormal mitotic Tau phosphorylation". These mitotic epitopes may allow for the nuclear depletion of Pin1. This latter may be responsible for escaping classical apoptosis in a subset of neurons. Since neurofibrillary degeneration is likely to be a third way to die, molecular mechanisms leading to changes in Tau phosphorylation including activation of kinases such as cdk5 or other regulators such as Pin1 could be important drug targets as they are possibly involved in early stages of neurodegeneration. 相似文献
74.
75.
Duodenal somatostatinomas associated with von Recklinghausen's neurofibromatosis. Apropos of 2 cases
Cohen C Heymann MF Michenet P Memeteau F Saint-Marc O Emy P Maitre F Le Bodic MF 《Annales de pathologie》2000,20(6):609-611
Somatostatinomas are rare neuroendocrine tumors; they are essentially located in the pancreas and in the duodenum. The association with a neurofibromatosis type I is especially observed when the tumor is located in the ampulla of Vater. These tumors are not associated with a "somatostatin syndrome", but often present with gastrointestinal bleeding, abdominal pain and obstructive jaundice. The diagnosis is confirmed by immunohistochemical studies. The aim of this study is to report 2 cases of metastazing duodenal periampullary somatostatinomas associated with von Recklinghausen's disease and to discuss the prognosis of these tumors. Future genetic research are necessary as point out the familial feature of this association in one of our cases. 相似文献
76.
高效液相色谱法测定寒痹停片中士的宁含量 总被引:6,自引:0,他引:6
目的:建立用HPLC测定寒痹停片中士的含量的方法。方法:氰基柱;流动相-甲醇-水-三乙胺-乙酸(9800:155:15:30);紫外检测波长254nm。结果:在4~20ug/ml范围内,标准曲线回归方程为:Y=-2803+8967x(r=0.9997),RSD=1.65%?加样回收率的平均值为99.82%。结论:实验表明,这是一个适用于生产控制和产品质量检验的简单、快速、准确的方法。 相似文献
77.
MF Whitfield 《Archives of disease in childhood》1980,55(2):139-142
A third case of chondrodysplasia punctata after exposure to warfarin alone in early pregnancy is described. The clinical course of the child during the first 18 months is outlined. The use of warfarin in early pregnancy must be avoided because of its established teratogenic effects in causing this syndrome, in addition to an overall increase in perinatal mortality. 相似文献
78.
Posteroanterior (PA) and caudally angulated PA views were obtained in 20 patients undergoing routine coronary arteriography. Although the left main coronary artery (LMCA) was seen well on both views in all patients, the PA-caudal view improved depiction of the LMCA bifurcation in 15 (75%). In addition, the PA-caudal view markedly improved depiction of the circumflex artery, affording optimal depiction of this artery and its branches in 78%-89% of patients. Neither the PA nor the PA-caudal view allowed adequate depiction of the left anterior descending artery. Thus, the PA-caudal view should supplant the PA view in routine coronary arteriography. 相似文献
79.