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991.
Helena Gil-Peña Natalia Mejia Oscar Alvarez-Garcia Vanessa Loredo Fernando Santos 《Pediatric nephrology (Berlin, Germany)》2010,25(4):733-737
Growth retardation remains a major complication in children with primary tubular disorders, despite adequate supplemental
treatment with electrolytes, water and bicarbonate. Chronic hypokalemia, characteristic of some tubulopathies, impairs growth
by mechanisms that are not well known. Association with growth hormone deficiency has been reported in patients with Bartter’s
or Gitelman’s syndrome. Tissue-specific alterations of growth hormone and insulin-like growth factor I axis have been described
in experimental models of potassium depletion. Hypokalemic rats gain less body length and weight than pair-fed normokalemic
animals and, by contrast, develop renal hypertrophy. These rats have low circulating concentrations of insulin-like growth
factor I, depressed messenger ribonucleic acid (mRNA) levels of this peptide in the tibial growth plate, and they are resistant
to the longitudinal growth-promoting effects of exogenous growth hormone. The reason for this resistance remains to be defined.
No alterations in the intracellular signaling for growth hormone have been found in the liver of hypokalemic rats. However,
treatment with high doses of growth hormone is unable to normalize hypertrophy of the epiphyseal cartilage chondrocytes, which
are severely disturbed in potassium depletion and likely play an important role in the pathogenia of growth impairment in
this condition. 相似文献
992.
993.
Koul H Huh JS Rove KO Crompton L Koul S Meacham RB Kim FJ 《American journal of cancer research》2011,1(2):240-254
Renal cell carcinoma (RCC) is a disease in which cancer cells form in the tubules of the kidney. RCC, the incidence of which is increasing annually, represents five percent of adult epithelial cancers. Clear cell carcinoma represents the most frequent histological subtype. RCC is characterized by a lack of early warning signs, diverse clinical manifestations. Incidentally detected tumors in asymptomatic individuals have been steadily increasing owing to the increased usage of various imaging technologies. Currently there are no recommendations for screening to detect and make an early diagnosis of renal cancer. But in recent years, the discovery of new molecular and cytogenetic markers has led to the recognition and classification of several novel subtypes of RCC, and the introduction of molecular-targeted therapy for advanced-stage RCC. We performed a literature review using PubMed and discuss current knowledge of epidemiology, pathophysiology, evaluation, treatment, and future research directions of RCC. 相似文献
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997.
Interaction of FkpA, a peptidyl-prolyl cis/trans isomerase with EspP autotransporter protein 总被引:1,自引:0,他引:1
The serine protease autotransporters of Enterobacteriaceae (SPATEs) represent a large class of proteases with contributions to virulence. They are synthesized with a C-terminal domain that forms a β-barrel pore in the outer membrane implicated in translocation of the N-terminal 'passenger' domain across the outer membrane. The most recent model for autotransporter secretion comprises entry to the periplasm via the Sec apparatus, followed by the insertion of the C-terminus into the outer membrane as a β-barrel protein and accompanied by translocation of the passenger domain to the bacterial cell surface, all of this with the assistance of the Bam complex insertase/foldase and periplasmic chaperone proteins. We have recently observed direct involvement of periplasmic chaperones in the biogenesis of EspP, a prototypical autotransporter protein produced by Escherichia coli O157:H7. Using molecular and biophysical approaches we demonstrated for the first time, direct protein-protein interactions between the periplasmic SurA and DegP chaperones and either the EspP-β or EspP passenger domains. Such chaperone interactions took place on conserved aromatic residues on the SPATE family. In this report, we now demonstrate direct binding of the periplasmic chaperone FkpA to the EspP passanger domain in Surface Plasmon Resonance experiments with relatively high affinity. We also provide evidence of interaction between the SurA and Skp chaperones with the Bam. These findings in conjunction with newly published data support the role of chaperones in preventing misfolding of AT passenger domains before translocation throughout the Bam complex. 相似文献
998.
Aline P.M. Dias Fernando N. Tavares Eliane V. Costa Edson E. da Silva 《Journal of clinical virology》2009,44(4):337-340
BackgroundThe virological surveillance of acute flaccid paralysis (AFP) is a critical component of the initiative of the World Health Organization (WHO) to eradicate poliomyelitis worldwide. Furthermore rapid methods are needed either to detect or rule out the presence of polioviruses during the late stages of eradication, especially in polio-free areas.ObjectivesThe aim of this study was to evaluate a fast protocol combining one passage (5 days) in cell culture followed by RT-PCR and molecular typing in order to detect and type poliovirus (PV) and other enteroviruses associated with AFP cases.Study designA total of 216 fecal suspensions from AFP suspected cases were tested by using this approach and compared with the WHO gold standard.ResultsUsing the WHO protocol enterovirus was detected in 12 out of the 216 AFP samples (5.55%) while with the proposed protocol enterovirus was detected in 15 out of the 216 AFP samples (6.94%). The additional positive samples detected by the proposed method were classified as non-polio enteroviruses (NPEV).ConclusionsThe proposed protocol showed higher sensitivity than the WHO gold standard, reducing the entire process of identification and typing of the isolates from the typically 14–21 days to only ~6–8 days. 相似文献
999.
Konsta Duesing Guillaume Charpentier Michel Marre Jean Tichet Serge Hercberg Beverley Balkau Philippe Froguel Fernando Gibson 《BMC medical genetics》2009,10(1):13
Background
APOA2 is a positional and biological candidate gene for type 2 diabetes at the chromosome 1q21-q24 susceptibility locus. The aim of this study was to examine if HapMap phase II tag SNPs in APOA2 are associated with type 2 diabetes and quantitative traits in French Caucasian subjects. 相似文献1000.