抗前列腺增生药SL—89．0519—08的合成

目的：研究治疗前列腺增生的α1－受体拮抗剂SL－89．0519－08的合成路线。方法：以5－氯－2－甲氧基苯胺为原料，经环合，烃化，脱保护基和亲核取代等步骤，合成了目标化合物，同时对原料合成工艺进行了摸索。结果：合成的目标化合物经IR，^1HMNR,EI-MS和HRMS得以确证。结论：该合成工艺和路线适宜工业化生产。     

腹腔镜在腹股沟疝修补术中的应用

目的：探讨腹腔镜在腹股沟疝修补手术中的优缺点，方法：31例腹股沟疝行腹腔镜疝修补术，其中斜疝25例，直疝6例，复发疝4例，均行腹腔内术式（TAPP）。结果：手术全部完成，平均手术时间61min(40-95min),无中转手术，4例同时行胆囊切除术，平均住院7．5d，术后近期随访无复发，结论：腹腔镜疝修补术是一种安全的手术，术后恢复时间短，复发率低。     

L-arginine对局灶脑缺血脑细胞凋亡和微血管密度的影响

目的探讨NO合成底物左旋-精氨酸(L-Arg)对兔局灶脑缺血后血管再生和脑细胞凋亡的影响。方法兔局灶脑缺血后应用L-Arg,流式细胞仪定量分析细胞凋亡率的变化,CD34免疫组织化学测脑组织微血管密度(MVD),脑组织含水率评价脑水肿。结果与对照组比较,L-Arg组脑细胞凋亡率明显减少(8.72±2.62 vs 16.62±2.82,P<0.01),同时脑组织MVD却明显增加(1.21±0.43 vs 0.69±0.22,P<0.01)。结论外源性L-Arg可减少缺血后脑细胞凋亡并促进缺血后血管再生,对局灶脑缺血具有重要的神经保护作用。     

^1H-magnetic resonance spectroscopy screening for animals with acute cerebral infraction suitable forthrombolytic therapy

BACKGROUND: As a non-invasive technique which can provide comprehensive biological information, 1H-magnetic resonance spectroscopy (1H-MRS) may provide valuable reference data for irreversible recovery or reversible changes in ischemic tissue after stroke. OBJECTIVE: To monitor and evaluate the effect of the urokinase thrombolytic therapy after experimental acute cerebral ischemia by 1H-MRS technology and investigate its adaptability. DESIGN: Randomly controlled animal study. SETTINGS: Shenzhen Hospital of Peking University and National Key Laboratory of Pattern and Atom & Molecular Physics, Wuhan Physics and Mathematics Institute, Chinese Academy of Science. MATERIALS: Eleven healthy adult Sprague-Dawley (SD) rats, weighing 260–300 g and of both genders, were supplied by Experimental Animal Center of Tongji Medical Collage, Huazhong University of Science and Technology [SCXK (e) 2004-007]. 4.7T superconducting nuclear magnetic resonance meter was provided by Brucker Company. METHODS: The experiment was carried out in Shenzhen Hospital of Peking University and National Key Laboratory of Pattern and Atom & Molecular Physics, Wuhan Physics and Mathematics Institute, Chinese Academy of Science from August 2003 to December 2005. ① The rats were randomly divided into 30-minute self-thrombo-embolism group (n =6) and 60-minute self-thrombo-embolism group (n =5). Six rats in 30-minute self-thrombo-embolism group were occluded with clot embolus for 30 minutes and 5 rats in 60-minute self-thrombo-embolism group were occluded for 60 minutes. 10 000 U/kg urokinase was dissolved in 2 mL saline and the operation lasted for 5 minutes. ② 1H-MRS was performed before thrombolysis and at 3 hours and 24 hours after successful embolization. The metabolic changes of N-acetyl-L-aspartic acid (NAA)/phosphocreatine (PCr) + creatine (Cr), choline phosphate (Cho)/PCr+Cr and lactic acid (Lac)/PCr+Cr in the region of interests were analyzed. ③ The T2W image was conducted 24 hours after the thrombolytic therapy with TR=500 ms and TE=25 ms. ④ The subjects were sacrificed immediately after 1H-MRS and the brain tissues were cut into pieces and stained with HE method; in addition, pathological changes were observed under optic microscope. MAIN OUTCOME MEASURES: ① Metabolic changes of NAA/PCr+Cr, Cho/PCr+Cr and Lac/PCr+Cr in the region of interests; ② T2W image at 24 hours after the thrombolysis; ③ pathological observation of brain tissue. RESULTS: Eleven rats were all involved in the final analysis. ① Metabolic changes in the region of interests : In 30-minute self-thrombo-embolism group, the Lac peak emerged immediately after the embolism, but the ischemic zone decreased 3 hours after the thrombolytic therapy (0.252±0.01, 0.603±0.01, P < 0.01). Lac/(PCr+Cr) ratio was 0.290±0.01 at 24 hours after thrombolysis, which was higher than that at 3 hours after thrombolysis (P < 0.01). The NAA/ (PCr+Cr) ratio decreased significantly at 3 hours after the thrombolysis as compared with that before thrombolysis (0.922±0.16, 1.196±0.01, P < 0.05). In 60-minute self-thrombo-embolism group, the Lac/(PCr+Cr) ratio was higher at 3 hours after thrombolysis than that before thrombolysis (0.846±0.12, 0.601±0.11, P < 0.05) and the NAA/(PCr+Cr) decreased at 3 hours after the embolism. Fluctuation of NAA/ (PCr+Cr) ranged from 0.68 to 0.75 before thrombolysis and from 0.71 to 0.75 at 3 hours after thrombolysis. ② T2W image: T2W image showed that 2 subjects in 30-minute self-thrombo-embolism group whose Lac/NAA was higher than 0.7 suffered from intracranial hemorrhage. This meant that the subjects with Lac/NAA > 0.7 were more likely to suffer from intracranial hemorrhage. ③ Histological and morphological examinations: Optic microscope demonstrated that interspace surrounding nerve cells was widened at ischemic center; neurons were swelling; nucleus was stained lightly; pyknosis and mesenchymal edema were mainly observed in lateral cortex of brow and vertex and in lateral part of corpus striatum. CONCLUSION: ①Compound parameters in ischemic area before thrombolysis should be regarded as an important predicting marker for thrombolytic therapy, effect evaluation and termination. ② 1H-MRS combining with other imaging technique is a detecting way for screening cases who are suitable for thrombolytic therapy.     

桂林市传染病网络直报工作第一年效果分析

目的观察传染病网络直报系统启动一年来的传染病监测质量,探讨网络直报评价方法.方法描述研究网络直报工作效果.结果实行网络直报后,传染病从临床医生发现到疾病预防控制机构接到报告的平均时间由5.48 d缩短至1.67 d,报卡数量增加20.84%,21.03%的报告卡得到校正,网络直报覆盖率县辖区医院为96.55%,市城区为69.23%,79.21%的直报单位有专用电脑.结论网络直报的启用及其系统的建立,提高了桂林市传染病疫情报告的质量和水平,同时应尽快建立与之相应的疫情报告管理方法和标准.     

胰岛素瘤的诊断和治疗：附137例报告

目的总结胰岛素瘤的诊断方法和外科治疗效果。方法回顾性分析近26年来收治的胰岛素瘤137例患者的临床资料。结果男77例,女60例。137例均具有典型的Whipple三联症。B超,CT,MRI检查的阳性率分别为35.1%,67.9%,58.1%。126例手术治疗患者中,102例(80.9%)行胰岛素瘤摘除术,4例(3.2%)行胰十二指肠切除术,16例(12.7%)行胰体尾切除术,另外4例(3.2%)行开腹探查术。切除的122例肿瘤中,良性占96.7%,恶性3.3%;单发肿瘤98.4%,多发1.6%。86.9%肿瘤直径≤2.0cm。13.1%肿瘤位于胰头;位于胰体和胰尾者分别为46.7%和40.2%。结论Whipple三联症,结合IRI,IGR,C-肽,以及胰岛素原的检测水平,可作为胰岛素瘤的定性诊断依据。胰岛素瘤的定位诊断仍很困难,联合应用多种影像学检查方法有助于提高检出率。治疗首选肿瘤摘除术,如果术中探查未能找到肿瘤,不宜盲目行胰体尾切除术,应排除其他原因所致低血糖症,同时进一步完善影像学检查方法,明确定位后,再考虑二次手术治疗。     

兔内听动脉阻断与听觉改变的关系

目的 探讨实验性内听动脉阻断与术后听力改变的关系及术中听觉监控的方法。方法 实验兔乙状窦后进路暴露小脑桥脑角，下压小脑暴露内听道及第Ⅷ神经复合体，分辨出内听动脉，予阻断不同时间，分别行蜗神经直接复合动作电位(DCAP)和畸变产物耳声发射记录(DPOAE)。结果对照组DCAPN1波潜伏期值无明显改变。内耳血供阻断10s和30s组在压迫开始后3min，DCAPN1波潜伏期值均恢复为初始值。内耳血供阻断1min组在压迫开始后3h，DCAPN1波潜伏期值未恢复为初始值假手术组DPOAE幅值无明显改变，内耳血供阻断10s、30s对DPOAE幅值无影响，内耳血供阻断1min使得DPOAE幅值下降结论兔内耳血供阻断1min可能造成兔听觉不可逆损伤。DCAP、DPOAE能有效、持续地监控内耳血供阻断，是耳神经外科手术中实用的听觉监测手段。     

应用VTK技术建立唇腭裂可视化模型

目的:寻求一种高效率、高精度建立唇腭裂患者颌面部可视化模型的方法.方法:利用薄层CT获取原始数据,运用k-均值聚类算法进行图像分割,结合VTK(Visualization Toolkit)技术进行可视化模型的建立.结果:建立了更为精确的唇腭裂患者颌面部的可视化模型,总结出了一种更为方便高效的通用可视化建模方法.结论:应用VTK方法建立可视化模型,软件开发周期更短,精度更高,应用更广泛.     

微型钢板与克氏针治疗掌指骨骨折的疗效对比分析

目的：探讨应用微型钢板及克氏针治疗各种掌指骨骨折的不同疗效。方法：47例（62处）掌指骨骨折。其中开放性骨折19例（24处），闭合性骨折28例（38处），合并严重软组织损伤或肌腱损伤10例（13处）。分为微型钢板固定组27例（34处）及克氏针组20例（28处）。采取伤口清创，微型解剖钢板螺丝钉系统内固定术及克氏针治疗。对比其疗效。结果：全部病例骨性愈合，以TAM为评定标准，微型钢板组中优为20例，良10例，优良率为88．2％。克氏针组中优为12例，良7例，优良率为67．8％。结论：在掌握好适应证的前提下，使用微型钢板切开复位内固定治疗各种类型的掌指骨骨折术后骨折愈合时间、关节功能的恢复程度及伤口感染情况明显优于克氏针组。     

Ovid医学信息平台在医学科研中的应用

详细介绍了Ovid 医学信息平台的特点及功能,并对其数据库所涉及学科作了介绍,指出Ovid 医学信息平台以其超大容量、完美服务及快捷查阅等特点优于同类软件,可以为医学科研人员提供高质量、深层次、全方位的服务.