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SH Opdal TO Rognum Å Vege AK Stave BM Dupuy T Egeland 《Acta paediatrica (Oslo, Norway : 1992)》1998,87(10):1039-1044
The purpose of the present study was to investigate substitutions in the D-loop of mitochondrial DNA (mtDNA) in sudden infant death syndrome (SIDS) and controls, since several observations indicate the involvement of mtDNA mutations in SIDS. These include elevated levels of vitreous humour hypoxanthine in SIDS victims, familial clustering without mendelian traits, and observations of increased sleepiness and a lower activity score in infants who later succumbed to SIDS. Eighty-two cases of SIDS and 133 controls were investigated and the D-loop sequences were recorded in the base-pair range 16 055-16 500 in the mtDNA sequence. The sequencing was carried out using the Applied Biosystems Sequenase dye terminator method and a ABD373A sequencer. The recorded D-loop sequences were compared with the Cambridge sequence and differences were recorded as substitutions. The SIDS cases had a tendency towards a higher substitution rate in the D-loop than the controls ( p = 0.088). This observation makes it interesting to search for deleterious mutations in other locations in the mtDNA. 相似文献
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Facial nerve neuromas: radiologic evaluation 总被引:3,自引:0,他引:3
Latack JT; Gabrielsen TO; Knake JE; Kemink JL; Graham MD; Gebarski SS; Yang PJ 《Radiology》1983,149(3):731
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A. AL‐MAWALI L. B. TO D. GILLIS P. HISSARIA J. MUNDY I. LEWIS 《International journal of laboratory hematology》2009,31(1):61-68
Immunophenotyping of acute myeloid leukaemia (AML) has controversial implications with regards to prognosis. The aims of the present study were to determine the frequency of leukaemia‐associated phenotypes (LAP) in AML and to correlate their presence with response to induction chemotherapy. We analysed bone marrow samples at diagnosis from 84 AML patients using triple staining flow cytometry with routine standard panel of monoclonal antibodies. The association of LAP and response to induction chemotherapy was evaluated retrospectively. LAP were observed in 54 (64%) patients: lineage infidelity in 19 (35%), asynchronous antigen expression in 28 (52%), and lack of expected lineage specific antigens in 19 (35%). Significant correlation was found between LAP and responses to induction chemotherapy. Response to induction chemotherapy was more frequent in the absence of LAP (P < 0.05, estimated risk ratio of 1.6, 95%CI, 1.0–2.6) in a multivariate analysis. In conclusion, our data show the presence of LAP in AML is an independent predictor for response to induction chemotherapy and risk of relapse and should be considered for counselling patients and planning therapy. 相似文献
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CHENG Vincent Chi-Chung CHAN Jasper Fuk-Woo WONG Sally Cheuk-Ying CHEN Jonathan Hon-Kwan TAI Josepha Wai-Ming YAN Mei-Kum KWAN Grace See-Wai TSE Herman TO Kelvin Kai-Wang HO Pak-Leung YUEN Kwok-Yung 《中华医学杂志(英文版)》2013,126(23):4504-4509
Background Identification of hospitalized carbapenem-resistant Enterobacteriaceae (CRE)-positive patient is important in preventing nosocomial transmission.The objective of this study was to illustrate the implementation of proactive infection control measures in preventing nosocomial transmission of CRE in a healthcare region of over 3200 beds in Hong Kong between October 1,2010 and December 31,2011.Methods The program included active surveillance culture in patients with history of medical tourism with hospitalization and surgical operation outside Hong Kong within 12 months before admission,and "added test" as an opportunistic CRE screening in all fecal specimens submitted to the laboratory.Outbreak investigation and contact tracing were conducted for CRE-positive patients.Serial quantitative culture was performed on CRE-positive patients and the duration of fecal carriage of CRE was analyzed.Results During the study period,a total of 6533 patients were screened for CRE,of which 76 patients were positive (10 from active surveillance culture,65 from "added test",and 1 secondary case from contact tracing of 223 patients with no nosocomial outbreak),resulting in an overall rate of CRE fecal carriage of 1.2%.The median time of fecal carriage of CRE was 43 days (range,13-119 days).Beta-lactam-beta-lactamase-inhibitors,cephalosporins,and fluoroquinolones were associated significantly with high fecal bacterial load when used 90 days before CRE detection,while use of cephalosporins,carbapenems,and fiuoroquinolones after CRE detection are significantly associated with longer duration of carriage.The duration of fecal carriage of CRE also correlates significantly with the initial fecal bacterial load (Pearson correlation:0.53; P=0.02).Conclusion Proactive infection control measures by enhanced surveillance program identify CRE-positive patients and data obtained are useful for the planning of and resource allocation for CRE control. 相似文献
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